Rabbit anti scd1

Manufactured by Cell Signaling Technology

Sourced in United States

Rabbit anti-SCD1 is a primary antibody that specifically binds to the Stearoyl-CoA Desaturase 1 (SCD1) protein. SCD1 is an enzyme that catalyzes the rate-limiting step in the synthesis of monounsaturated fatty acids. This antibody can be used in various research applications to detect and study the SCD1 protein.

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Lab products found in correlation

Protease inhibitor cocktail, by Thermo Fisher Scientific (2 mentions) Ecl kit, by Thermo Fisher Scientific (2 mentions) Fa free bsa, by Merck Group (1 mentions) Mouse anti β actin, by Merck Group (1 mentions) Fetal calf serum (fcs), by Gemini Bio (1 mentions) Protease inhibitor cocktail, by Roche (1 mentions) Rabbit anti cox 2, by Abcam (1 mentions) Hrp conjugated anti rabbit secondary antibody, by Santa Cruz Biotechnology (1 mentions) Anti β actin, by Santa Cruz Biotechnology (1 mentions) Supersignal west pico chemiluminescent substrate kit, by Thermo Fisher Scientific (1 mentions) Rabbit anti fas, by Cell Signaling Technology (1 mentions) Rabbit anti fads2, by Abcam (1 mentions) Pvdf membrane, by Thermo Fisher Scientific (1 mentions) Rabbit anti atf4, by Cell Signaling Technology (1 mentions) Mouse anti actin, by Santa Cruz Biotechnology (1 mentions) Hrp conjugated secondary antibody, by Promega (1 mentions) Na ve cd4 t cell isolation kit, by Miltenyi Biotec (1 mentions) Bis tris gel, by Thermo Fisher Scientific (1 mentions) Nitrocellulose membrane, by Bio-Rad (1 mentions) Mouse anti gapdh, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Anti-SCD1 (15 citations)

4 protocols using rabbit anti scd1

Fatty Acid Metabolism and Protein Synthesis

Cited 1 time

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We obtained fatty acids (FAs) from Nu-Chek Prep, Inc.; radiolabeled S-35 methionine from Perkin-Elmer (cat. no. NEG709A500UC); SCD1 inhibitor (A939572; cat. no. 19123), Nonidet P-40 (NP-40; cat. no. 600009) and Protein Synthesis kit (cat. no. 601100) from Cayman Chemicals; rabbit anti-YB-1 from ProteinTech; mouse anti-β-actin from Millipore Sigma; rabbit anti-SCD1 from Cell Signal; YB-1-myc plasmid from Addgene; non-targeting control siRNA and YB-1 siRNAs from Qiagen; FA-free BSA from Sigma-Aldrich; Protease inhibitor cocktail from Roche; Fetal Calf serum (FCS) and Delipidated fetal calf serum (DFCS) from Gemini Bio. All FAs added into culture media were conjugated to BSA as previously reported (10 (link)).

Jeffords E., Freeman S., Cole B., Root K., Chekouo T., Melvin R.G., Bemis L, & Simmons GE J.r. (2020). Y-box binding protein 1 acts as a negative regulator of stearoyl CoA desaturase 1 in clear cell renal cell carcinoma. Oncology Letters, 20(5), 165.

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Western Blot Analysis of Lipid Metabolism Proteins

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Protein extracts of cells were prepared using RIPA buffer containing protease inhibitor cocktail, and protein concentrations were measured using a BCA kit (Pierce). One hundred micrograms of protein was resolved by electrophoresis in 8% SDS‐PAGE gel and blotted to PVDF membranes (Invitrogen). Membranes were incubated with indicated primary antibodies overnight at 4°C and then with an anti‐rabbit HRP‐conjugated secondary antibody (1:3000; Santa Cruz Biotechnology) for 2 hours at room temperature. The primary antibodies used in this study were rabbit anti‐FAS (1:1000; Cell signaling), rabbit anti‐SCD1 (1:500; Cell signaling), rabbit anti‐FADS2 (1:1000; Abcam), rabbit anti‐COX2 (1:1000; Abcam), and anti‐β‐actin (1:2000; Santa Cruz Biotechnology) as a loading control. HRP activity was measured using Supersignal West Pico Chemiluminescent Substrate kit (Thermo Fisher Scientific).

Luo H., Chen C., Li X., Zhang X., Su C., Liu Y., Cao T., Hao L., Wang M, & Kang J.X. (2021). Increased lipogenesis is critical for self‐renewal and growth of breast cancer stem cells: Impact of omega‐3 fatty acids. Stem Cells, 39(12), 1660-1670.

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Western Blot Analysis of TFH Cells

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Naïve CD4⁺ T cells were purified from WT or BCL6 KO mice with naïve CD4⁺ T-cell isolation kit (Miltenyi Biotec). Then, the cells were activated and cultured under the T_FH polarizing condition as above. The cultured cells were lysed in sample lysis buffer (Cell Signaling Technology) with a protease inhibitor cocktail (Thermo Scientific) and incubated on ice for 30 min. Proteins in the supernatants were heat denatured, separated by 4% to 12% gradient Bis-Tris gel (Invitrogen) and transferred into a nitrocellulose membrane (Bio Rad). Membrane was blocked with 5% of nonfat milk in TBST for 1 h at RT. After washing with TBST (Tris-buffered saline with 0.1% Tween 20), membrane was incubated with following primary antibodies for overnight: Rabbit-anti SCD1 (Cell signaling), rabbit-anti ATF4 (Cell signaling) or mouse-anti actin (Santa Cruz). Membrane was then rinsed with TBST and incubated with the HRP-conjugated secondary antibodies (Promega) for 1 h at RT. ECL kit (Thermo) was used for film (Fisher Scientific) development.

Son Y.M., Cheon I.S., Goplen N.P., Dent A.L, & Sun J. (2020). Inhibition of stearoyl-CoA desaturases suppresses follicular help T- and germinal center B- cell responses. European journal of immunology, 50(7), 1067-1077.

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Protein Extraction and Western Blot Analysis

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The cells were lysed with RIPA buffer (Beyotime, Shanghai, China) containing 1X PMSF and a protease inhibitor cocktail. After centrifugation at 4 °C and 12,000× g for 15 min, the concentration of proteins in the supernatant was determined using a Piece BCA protein assay kit (Thermo Fisher Scientific Inc., Waltham, MA, USA). The proteins were separated using 10% sodium dodecyl-sulfate-polyacrylamide gels. Thereafter, they were transferred onto a PVDF membrane using a transfer apparatus, and after blocking with 5% skimmed milk, the membrane was incubated with the primary antibody overnight at 4 °C. The primary antibodies, rabbit anti-EGFR, rabbit anti-phospho-EGFR, rabbit anti-FASN, rabbit anti-LIPIN1, rabbit anti-Insig1, rabbit anti-SCD1, rabbit anti-SREBP1, rabbit anti-pAMPK, rabbit anti-p-AKT, rabbit anti-p-PI3K, and mouse anti-GAPDH, were purchased from Cell Signaling Technology (Danvers, MA, USA). Further, rabbit anti-Srebf1 was purchased from Abcam (Cambridge, MA, USA), while goat anti-rabbit IgG and goat anti-mouse secondary antibodies were purchased from Proteintech (Rosemont, IL, USA).

Liao T., Deng J., Chen W., Xu J., Yang G., Zhou M., Lv Z., Wang S., Song S., Tan X., Yin Z., Li Y, & Jin Y. (2022). Fasudil Increased the Sensitivity to Gefitinib in NSCLC by Decreasing Intracellular Lipid Accumulation. Cancers, 14(19), 4709.

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