Human breast cancer cell lines MCF10A, MDA-MB-468 (MDA-468), BT20, and MDA-MB-231 (MDA-231) were purchased from ATCC (Manassas, VA, USA) and cultured as instructed. Bone-metastatic MDA-MB-231BoM (231-BoM), and brain-metastatic MDA-MB-231BrM (231-BrM) cell lines are from the Massagué laboratory and were cultured in DMEM supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin [43 (link), 44 (link)]. Trastuzumab-resistant BT474 (BT474-TtzmR) were a kind gift from Dr. Dihua Yu [45 (link)]. All cell lines have been authenticated using the standard method, and tested for mycoplasma. If tested positive for mycoplasma contamination, cells were treated until free of contamination before use. Pacritinib, ruxolitinib, sonidegib, and vismodegib were obtained from AdooQ Bioscience (Irvine, CA, USA) and stock solutions were prepared using dimethyl sulfoxide (DMSO) for administration in cell culture.
Sonidegib
Manufactured by Adooq
Sourced in United States
Sonidegib is a lab equipment product that serves as a small-molecule inhibitor. Its core function is to target and inhibit the Hedgehog signaling pathway.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using sonidegib
1
Breast Cancer Cell Lines and Inhibitors
2
Breast Cancer Cell Lines and Inhibitors
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Human breast cancer cell lines MCF10A, MDA-MB-468 (MDA-468), BT20, and MDA-MB-231 (MDA-231) were purchased from ATCC (Manassas, VA, USA) and cultured as instructed. Bone-metastatic MDA-MB-231BoM (231-BoM), and brain-metastatic MDA-MB-231BrM (231-BrM) cell lines are from the Massagué laboratory and were cultured in DMEM supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin [43 (link), 44 (link)]. Trastuzumab-resistant BT474 (BT474-TtzmR) were a kind gift from Dr. Dihua Yu [45 (link)]. All cell lines have been authenticated using the standard method, and tested for mycoplasma. If tested positive for mycoplasma contamination, cells were treated until free of contamination before use. Pacritinib, ruxolitinib, sonidegib, and vismodegib were obtained from AdooQ Bioscience (Irvine, CA, USA) and stock solutions were prepared using dimethyl sulfoxide (DMSO) for administration in cell culture.
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