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Cobas amplicor hiv 1 monitor test version 1

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The Cobas Amplicor HIV-1 Monitor Test, version 1.5, is a quantitative in vitro diagnostic test used for the measurement of human immunodeficiency virus type 1 (HIV-1) RNA in human plasma.

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Lab products found in correlation

Murex hiv 1.2 o hiv enzyme immunoassay, by DiaSorin (4 mentions) M2000 real time hiv 1 assay, by Abbott (3 mentions) Facsflow, by BD (3 mentions) Ficoll histopaque, by Merck Group (2 mentions) Macro vuetmrpr card tests, by BD (2 mentions) Hiv 1 2 unif 2 plus o enzyme immunoassay, by bioMérieux (2 mentions) Facscalibur, by BD (2 mentions) Facscalibur flow cytometer, by BD (2 mentions) Architect i1000sr, by Abbott (1 mentions) Vironostika hiv 1 2 unif 2 plus o enzyme immunoassay, by bioMérieux (1 mentions) Trucount tube, by BD (1 mentions) Cobas taqman 48 analyzer, by Roche (1 mentions)

16 protocols using cobas amplicor hiv 1 monitor test version 1

1

Serological Markers for Viral Infections

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HBV surface antigen (HBsAg) and anti-HCV antibodies were detected using an HBsAg radioimmunoassay (ARCHITECT i1000SR; Abbott, Abbott Park, IL, USA) and anti-HCV ELISA kit, respectively. The plasma HIV RNA load and CD4 cell count were quantified using a Cobas Amplicor HIV-1 monitor test, version 1.5 (Roche Diagnostics, Indianapolis, IN, USA) and FACSFlow (BD Biosciences, Franklin Lakes, NJ, USA), respectively.
Weng Y.W., Chen I.T., Tsai H.C., Wu K.S., Tseng Y.T., Sy C.L., Chen J.K., Lee S.S, & Chen Y.S. (2019). Trend of HIV transmitted drug resistance before and after implementation of HAART regimen restriction in the treatment of HIV-1 infected patients in southern Taiwan. BMC Infectious Diseases, 19, 741.
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2

Longitudinal Study of HIV-1 Subtype C Infection

Cited 3 times
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The Sinikithemba cohort comprised of 450 antiretroviral naïve, HIV-1 subtype C chronically adults enrolled from McCord Hospital (Durban, South Africa) from August 2003 to 2008 and followed up longitudinally as previously described [36 (link)–38 (link)]. The time of infection for these participants is unknown. Sociodemographic characteristics, plasma viral load and CD4 count measurements were obtained at baseline. CD4 counts and viral loads were measured every 3 and 6 months from enrolment, respectively. Viral loads were determined using automated Cobas Amplicor HIV-1 Monitor test version 1.5 (Roche Diagnostics, Rotkreuz, Switzerland) and CD4+ T cells were enumerated using the Multitest kit CD4/CD3/CD8/CD45 on a FACSCalibur flow cytometer (Becton Dickinson). The Masibambisane cohort comprised of HIV negative women recruited from antenatal clinics in Durban [39 (link)]. Peripheral blood mononuclear cells (PBMCs) from participants were isolated by Ficoll-Histopaque (Sigma) density gradient centrifugation from blood within 6 h of phlebotomy and frozen in liquid nitrogen until use.
Mlimi H., Naidoo K.K., Mabuka J., Ndung’u T, & Madlala P. (2022). Bone marrow stromal antigen 2 (BST-2) genetic variants influence expression levels and disease outcome in HIV-1 chronically infected patients. Retrovirology, 19, 3.
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3

Home-based HIV Testing and Counseling Protocol

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As a service to study participants, respondents were given the opportunity to learn their HIV status within their homes through home-based testing and counseling using venous blood samples collected for the survey or a separate capillary sample. Methods for home-based testing and counseling were consistent with national guidelines for HIV testing.14 All specimens for centralized testing were transported from the field to the National HIV Reference Laboratory, where HIV tests were performed. Specimens were screened with Vironostika HIV-1/2 UNIF II Plus O Enzyme Immunoassay (BioMérieux, Marcy l’Etoile, France). Specimens testing negative by the screening assay were reported as a final result negative. Specimens testing positive by the screening assay were confirmed using the Murex HIV.1.2.O HIV Enzyme Immunoassay (DiaSorin SpA, Saluggia, Italy). Samples showing discordant results after confirmatory testing were tested again with the 2 assays. Polymerase chain reaction (Cobas AmplicorHIV-1 Monitor Test, version 1.5; Roche Molecular Diagnostics, Pleasanton, CA) was performed to resolve specimens with twice-discordant results. All positive specimens and 5% of negative specimens were retested for quality assurance purposes using the same testing algorithm at the Kenya Medical Research Institute Laboratory.
Galbraith J.S., Ochieng A., Mwalili S., Emusu D., Mwandi Z., Kim A.A., Rutherford G., Maina W.K., Kimanga D.O., Chesang K, & Cherutich P. (2014). Status of Voluntary Medical Male Circumcision in Kenya: Findings From 2 Nationally Representative Surveys in Kenya, 2007 and 2012. Journal of acquired immune deficiency syndromes (1999), 66(Suppl 1), S37-S45.
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4

HIV Antibody Testing Protocol

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Blood specimens were tested for HIV antibody at NHRL using Vironstika HIV-1/2 Enzyme Immunoassay (BioMerieux, Marcy l’Etoile, France) as the screening assay and Murex HIV.1.2.0 Enzyme Immunoassay (DiaSorin, SpA, Saluggia, Italy) as the confirmatory assay. Specimens that tested HIV-negative by the screening assay were classified as HIV-negative. Specimens that tested HIV-positive by the screening assay were tested by the confirmatory assay, and, if they tested positive on the confirmatory assay, they were classified as HIV-positive. Specimens that were discordant on the screening and confirmatory assays were re-tested using the same testing algorithm. If results remained discrepant, polymerase chain reaction (Cobas Amplicor HIV-1 Monitor Test, version 1.5; Roche Molecular Diagnostics, Pleasanton, CA) was used to determine the final result.
Sirengo M., Muthoni L., Kellogg T.A., Kim A.A., Katana A., Mwanyumba S., Kimanga D.O., Maina W.K., Muraguri N., Elly B, & Rutherford G.W. (2014). Mother-to-Child Transmission of HIV in Kenya: Results From a Nationally Representative Study. Journal of acquired immune deficiency syndromes (1999), 66(Suppl 1), S66-S74.
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5

Serological Tests for Syphilis and HIV

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Serological tests for syphilis included the RPR test (BD Macro-VueTMRPR Card tests, USA) and T. pallidum particle agglutination test (FTI-SERODIA-TPPA. Fujirebio Taiwan Inc., Taoyuan, Taiwan). Plasma HIV RNA loads and CD4 lymphocyte counts were quantified with the use of the Cobas Amplicor HIV-1 Monitor™ Test, version 1.5, (Roche Diagnostics Corporation, Indianapolis, USA) and FACSFlow (Becton Dickinson), respectively.
Yang C.J., Lee N.Y., Chen T.C., Lin Y.H., Liang S.H., Lu P.L., Huang W.C., Tang H.J., Lee C.H., Lin H.H., Chen Y.H., Ko W.C, & Hung C.C. (2014). One Dose versus Three Weekly Doses of Benzathine Penicillin G for Patients Co-Infected with HIV and Early Syphilis: A Multicenter, Prospective Observational Study. PLoS ONE, 9(10), e109667.
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6

Serological Assays for Syphilis and HIV

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Serological tests for syphilis were performed with the use of rapid RPR test (BD Macro-VueTMRPR Card tests, USA) and Treponema pallidum hemagglutination test (FTI-SERODIA-TPPA. Fujirebio Taiwan Inc., Taoyuan, Taiwan) in the participating hospitals. Plasma HIV RNA load and CD4 lymphocyte count were quantified by the Cobas Amplicor HIV-1 Monitor™ Test, version 1.5, (Roche Diagnostics Corporation, Indianapolis, USA) and FACSFlow (Becton Dickinson), respectively.
Tsai J.C., Lin Y.H., Lu P.L., Shen N.J., Yang C.J., Lee N.Y., Tang H.J., Liu Y.M., Huang W.C., Lee C.H., Ko W.C., Chen Y.H., Lin H.H., Chen T.C, & Hung C.C. (2014). Comparison of Serological Response to Doxycycline versus Benzathine Penicillin G in the Treatment of Early Syphilis in HIV-Infected Patients: A Multi-Center Observational Study. PLoS ONE, 9(10), e109813.
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7

Quantifying HIV and HCV Viral Loads

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HIV and HCV viral loads were measured among HIV-mono-infected individuals and HIV/HCV-co-infected individuals. The plasma HIV RNA level was measured with quantitative reverse polymerase chain reaction (COBAS AMPLICOR HIV-1 Monitor Test version 1.5, Roche Molecular Systems, Branchburg, NJ, USA) following the manufacturer's instructions. The kit detects HIV-1 RNA ranges over 48 to 10,000,000 copies/ml. Plasma HCV RNA was quantified by commercial quantitative RT-PCR kit (Shenzhen PG Biotech Co., Ltd. Shenzhen, China), with a detection linear range of 103–107 IU/ml. Dilution was conducted whenever necessary.
He L., Zhao J., Wang M.H., Siu K.K., Gan Y.X., Chen L., Zee B.C., Yang L., Kung H.F., Yang Z.R, & He M.L. (2014). Interleukin-27 Is Differentially Associated with HIV Viral Load and CD4+ T Cell Counts in Therapy-Naïve HIV-Mono-Infected and HIV/HCV-Co-Infected Chinese. PLoS ONE, 9(5), e96792.
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8

Absolute CD4 T-cell Counting and Viral Load

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Absolute CD4 T-lymphocyte counts were estimated in the whole blood by flow cytometry using BD Tritest CD3 FITC/CD4 PE/CD45 PerCP with BD Trucount tubes (BD Biosciences, San Jose, CA, USA) and plasma viral load (pVL) was estimated using COBAS Amplicor HIV-1 monitor test, version 1.5 (Roche, NJ, USA) as per the manufacturer’s guidelines.
Singh S., Sharma A, & Arora S.K. (2014). High Producer Haplotype (CAG) of -863C/A, -308G/A and -238G/A Polymorphisms in the Promoter Region of TNF-α Gene Associate with Enhanced Apoptosis of Lymphocytes in HIV-1 Subtype C Infected Individuals from North India. PLoS ONE, 9(5), e98020.
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9

Structured Treatment Interruption in HIV-1 Infected Children

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Two HIV-1 infected children with a viral load (VL) undetectable by HAART (<400 copies/ml), without immunosuppression according to the 1994 CDC classification [11 ] and HIV asymptomatic for at least the last twelve months were submitted to a STI program. HAART was interrupted for 4 weeks followed by 12 weeks on treatment, until completion of three interruption/restart cycles (4 weeks off/12 weeks on) as previously described [4 , 6 (link)]. The VL, CD4+ and CD8+ cell counts as well as the clinical status of the patients were evaluated after each interruption period and at 6 and 12 weeks after HAART was restarted. VL was assessed using the Cobas Amplicor HIV-1 Monitor test, version 1.5 (Roche Diagnostics, Branchburg, NJ, USA), the detection limit of which is 400 copies/ml. Lymphocyte counts were measured by standard flow cytometry using the BD Standard Simultest / IMK Lymphocyte Kit (BD Biosciences, San Jose, CA, USA). The study was approved by the research ethics committee at the Hospital de Especialidades No. 25 of the Instituto Mexicano del Seguro Social and written informed consent from the parents or guardians of each patient was obtained.
Vazquez-Guillen J.M., Palacios-Saucedo G.C., Rivera-Morales L.G., Garcia-Campos J., Ortiz-Lopez R., Noguera-Julian M., Paredes R., Vielma-Ramirez H.J., Ramirez T.J., Chavez-Garcia M., Lopez-Guillen P., Briones-Lara E., Sanchez-Sanchez L.M., Vazquez-Martinez C.A, & Rodriguez-Padilla C. (2016). Mutations Related to Antiretroviral Resistance Identified by Ultra-Deep Sequencing in HIV-1 Infected Children under Structured Interruptions of HAART. PLoS ONE, 11(1), e0147591.
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10

HIV Diagnostic Testing Algorithm Validation

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Biologic testing was performed at the National HIV Reference Laboratory. HIV testing was done using Kenya’s validated testing algorithm, which included screening with Vironostika HIV-1/2 UNIF II Plus O Enzyme Immunoassay (bioMérieux, Marcy d’Etoile, France). Positive samples were confirmed with the Murex HIV.1.2.O HIV Enzyme Immunoassay (DiaSorin, SpA, Saluggia, Italy). Discordant results were retested with the 2 assays. Twice discordant results, if they occurred, were tested using a polymerase chain reaction assay (Cobas Amplicor HIV-1 Monitor Test, version 1.5; Roche Molecular Diagnostics, Pleasanton, CA). For quality control, all positive specimens and 5% of negative specimens were retested using the same testing algorithm at the Kenya Medical Research Institute laboratory. For persons with positive HIV tests, measurements of CD4 cell counts (BD FACSCalibur Flow Cytometer; Becton Dickinson Biosciences, San Jose, CA) were performed centrally as well as measurement of HIV viral load (Abbott M2000 Real-Time HIV-1 Assay; Abbott Laboratories, Abbott Park, IL).
Mbithi A., Gichangi A., Kim A.A., Katana A., Weyenga H., Williamson J., Robinson K., Oluoch T., Maina W.K., Kellogg T.A, & De Cock K.M. (2014). Tuberculosis and HIV at the National Level in Kenya: Results From the Second Kenya AIDS Indicator Survey. Journal of acquired immune deficiency syndromes (1999), 66(Suppl 1), S106-S115.
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