Allyl glycidyl ether, iminodiacetic acid and 1,6-diamino hexane were purchased from Sigma-Aldrich (Bornem, Belgium); acrylamide, methylene-bis-acrylamide, TEMED, ammonium persulfate, glutaraldehyde, ethanolamine and iminodiacetic acid (IDA) were from Sigma (St.Louis, Mo); C10/10, XK16/20, XK26/20, XK50/20 columns and Äkta Pure chromatographic system are from GE Healthcare (Uppsala, Sweden); Vernier stainless steel temperature probe and Logger Pro software are from Vernier (Beaverton, OR).
Iminodiacetic acid
Manufactured by Merck Group
Sourced in United States
Iminodiacetic acid is a chemical compound commonly used in laboratory equipment. It functions as a chelating agent, capable of forming stable complexes with various metal ions. The core function of iminodiacetic acid is to bind and sequester specific metal ions, facilitating their removal or separation in analytical and purification processes.
Automatically generated - may contain errors
Lab products found in correlation
Epichlorohydrin, by Merck Group (3 mentions)
Sodium borohydride, by Merck Group (3 mentions)
Sodium periodate, by Merck Group (3 mentions)
Sodium chloride (nacl), by Merck Group (3 mentions)
Glutaraldehyde, by Merck Group (2 mentions)
Sodium hydroxide (naoh), by Merck Group (2 mentions)
Methylene bisacrylamide, by Merck Group (1 mentions)
Acrylamide, by Merck Group (1 mentions)
Ethanolamine, by Merck Group (1 mentions)
Ammonium persulfate, by Merck Group (1 mentions)
Temed, by Merck Group (1 mentions)
C10 10, by GE Healthcare (1 mentions)
Xk26 20, by GE Healthcare (1 mentions)
1 6 diaminohexane, by Merck Group (1 mentions)
Xk 16 20, by GE Healthcare (1 mentions)
Xk 50 20, by GE Healthcare (1 mentions)
Allyl glycidyl ether, by Merck Group (1 mentions)
Polydiallyldimethylammonium chloride, by Merck Group (1 mentions)
Bradford protein assay dye reagent, by Bio-Rad (1 mentions)
Acetone, by Merck Group (1 mentions)
14 protocols using iminodiacetic acid
1
Covalent Polymer Functionalization Protocol
2
Agarose Microbead-Based Enzyme Assay
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Agarose microbeads (50–150 μm
diameter) were purchased from Agarose Bead Technologies (Madrid, Spain).
Polyethyleneimine (PEI) solution in H2O (Mw ∼ 60 000, 50 wt %), polyallylamine (PAH)
solution in H2O (Mw ∼
65 000, 10 wt %), polydiallyldimethylammonium chloride (PDADMAC)
solution in H2O (Mw < 100 000,
35 wt %), pyridoxal 5′-phosphate hydrate (PLP, 98%), rhodamine
B isothiocyanate mixed isomers (RhB), acetone, 2-phenylethylamine
(PEA, 98%), iminodiacetic acid (IDA), albumin bovine serum standard
(BSA), and other reagents and solvents of analytical grade were purchased
from Sigma-Aldrich (St. Louis, IL). Nicotinamide adenine dinucleotide-reduced
sodium salt (NADH) and flavin mononucleotide sodium salt (FMN) were
purchased from GERBU Biotechnik GmbH (Heidelberg, Germany). Flavin
Adenine dinucleotide disodium salt hydrate (FAD, 94%) was purchased
from Cymit Quimica S.L. (Barcelona, Spain). Riboflavin (Rf, 98%) was
purchased from Acros Organics B.V.B.A. (Fair Lawn, New Jersey, United
States). Isopropyl-β-d -thiogalactopiranoside (IPTG,
100%) was purchased from Fisher Bioreagents. The Bradford protein
assay dye reagent was purchased from BIORAD (Biorad. Hercules, CA).
Clear bottom black and white microplates (96-well) were purchased
from Avantor (2021 VWR International, LLC). μ-Slides 8 well
glass bottom was purchased from Ibidi (Planegg, Germany).
diameter) were purchased from Agarose Bead Technologies (Madrid, Spain).
Polyethyleneimine (PEI) solution in H2O (Mw ∼ 60 000, 50 wt %), polyallylamine (PAH)
solution in H2O (Mw ∼
65 000, 10 wt %), polydiallyldimethylammonium chloride (PDADMAC)
solution in H2O (Mw < 100 000,
35 wt %), pyridoxal 5′-phosphate hydrate (PLP, 98%), rhodamine
B isothiocyanate mixed isomers (RhB), acetone, 2-phenylethylamine
(PEA, 98%), iminodiacetic acid (IDA), albumin bovine serum standard
(BSA), and other reagents and solvents of analytical grade were purchased
from Sigma-Aldrich (St. Louis, IL). Nicotinamide adenine dinucleotide-reduced
sodium salt (NADH) and flavin mononucleotide sodium salt (FMN) were
purchased from GERBU Biotechnik GmbH (Heidelberg, Germany). Flavin
Adenine dinucleotide disodium salt hydrate (FAD, 94%) was purchased
from Cymit Quimica S.L. (Barcelona, Spain). Riboflavin (Rf, 98%) was
purchased from Acros Organics B.V.B.A. (Fair Lawn, New Jersey, United
States). Isopropyl-β-
100%) was purchased from Fisher Bioreagents. The Bradford protein
assay dye reagent was purchased from BIORAD (Biorad. Hercules, CA).
Clear bottom black and white microplates (96-well) were purchased
from Avantor (2021 VWR International, LLC). μ-Slides 8 well
glass bottom was purchased from Ibidi (Planegg, Germany).
3
Exploring Chemical Synthesis and Modification
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d -Tagatose, d -galactose, l -arabinose, l -cysteine, epichlorohydrin, iminodiacetic acid (IDA), sodium periodate (NaIO4), isopropyl-1-thio-β-d -galactopyranoside (IPTG), ampicillin, kanamycin, carbazole crystalline, and agarose CL-4B were purchased from Sigma (Sigma-Aldrich, St. Louis, MO, USA). In addition, different analytical grade reagents from different trademarks were used.
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4
Immobilization and Characterization of Enzymes
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Agarose 4 BCL was purchased from Agarose Bead Technologies (Madrid, Spain). Epichlorohydrin, iminodiacetic acid, triethylamine, sodium borohydride, sodium periodate, 1-octanothiol, bovine serum albumin, O-nitro-phenyl-β-d -galactopyranoside (O-NPG), p-nitrophenyl proprionate (p-NPP), 4-nitrophenyl-β-d -glucopyranoside (p-NPG), and high molecular weight protein (Sigma MarkerTM, St. Louis, MO, USA) were purchased from Sigma (Sigma-Aldrich®, St. Louis, MO, USA). Glutaraldehyde solution (25%, v/v) and ethylenediamine were purchased from Alfa Aesar (Thermo Fisher Scientific®, Waltham, MA, USA). Octyl-Sepharose CL-4B was purchased from GE Healthcare Bio-Sciences (Uppsala, Sweden). The β-galactosidase from Kluyveromyces lactis (Lactozym pure 6500 L) (KlBgal) was kindly supplied by Novozymes A/S (Copenhagen, Denmark). The lipase from Candida rugosa (CRL) was purchased from Sigma (Sigma-Aldrich®, St. Louis, MO, USA). Overexpression and purification of lipase (LipC12) and β-glucosidase (EaBglA) were performed as previously described by Glogauer et al. [40 (link)] and Crespim et al. [47 (link)], respectively. All other chemicals used were of analytical grade.
5
Silica Gel Preparation and Characterization
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3-Glycidyloxypropyl)trimethoxysilane (GLYMO, ≥98%) and iminodiacetic acid (IDA, 98%) were obtained from Sigma-Aldrich (Buchs, Switzerland). Ethylenediaminetetraacetic acid disodium salt dihydrate (EDTA-Na2, ≥99%) was purchased from Fluka (Buchs, Switzerland). Nickel(II) sulfate hexahydrate (NiSO4·6H2O, ACS), sodium hydroxide pellets (NaOH, >99%) and sodium chloride (NaCl, ACS) were purchased from Merck (Darmstadt, Germany). Hydrochloric acid (HCl, Supra 35%) was ordered from Carl Roth (Karlsruhe, Germany). Deionized water was purified by Milli-Q® Reference water purification system (Merck Millipore, Darmstadt, Germany) and was specified by an electrical resistivity of 18.2 MΩcm.
Gas supply for AAS: Acetylene 2.6 (>99.6 vol%, Messer Austria, Gumpoldskirchen, Austria); compressed air.
The following 12 silica gels differing in pore and particle size were gained: Carl Roth (Karlsruhe, Germany), 35 Å (40–63 µm), 60 Å (20–45 µm), 60 Å (30–200 µm), 60 Å (35–70 µm), 60 Å (40–63 µm), 60 Å (200–500 µm), 150 Å (35–70 µm), 150 Å (70–200 µm), 1000 Å (35–70 µm), and 250 Å (40–63 µm); Fluka (Buchs, Switzerland), 60 Å (63–200 µm); and Sigma-Aldrich (Buchs, Switzerland), 100 Å (63–200 µm).
Gas supply for AAS: Acetylene 2.6 (>99.6 vol%, Messer Austria, Gumpoldskirchen, Austria); compressed air.
The following 12 silica gels differing in pore and particle size were gained: Carl Roth (Karlsruhe, Germany), 35 Å (40–63 µm), 60 Å (20–45 µm), 60 Å (30–200 µm), 60 Å (35–70 µm), 60 Å (40–63 µm), 60 Å (200–500 µm), 150 Å (35–70 µm), 150 Å (70–200 µm), 1000 Å (35–70 µm), and 250 Å (40–63 µm); Fluka (Buchs, Switzerland), 60 Å (63–200 µm); and Sigma-Aldrich (Buchs, Switzerland), 100 Å (63–200 µm).
6
Comprehensive Metabolite Identification Protocol
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All reagents employed were of analytical or MS grade. Acetonitrile, methanol, isopropanol, and formic acid were purchased from Thermo Fisher Scientific (Madrid, Spain). Water employed to prepare the running buffer was purified through a Milli-Q system from Millipore (Millipore, Madrid, Spain). Arginine, glutamic acid, pyroglutamic acid, tyrosine, alanine, histidine, cystine, proline, aspartic acid, iminodiacetic acid, 5-hydroxy-L-tryptophan, and 5,6-dihydro-5-methyluracil were acquired in Sigma Aldrich (Madrid, Spain). Methionine sulfone and tyramine used as IS and sodium hydroxide were from Sigma Aldrich while purine and hexakis(2,2,3,3-tetrafluoropropoxy)phosphazine (HP-921) solutions used as a standard mass reference were acquired from Agilent Technologies (Waldbronn, Germany).
7
Functionalization of Pen G with GMA
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Pen G sodium salt, glycidyl methacrylate (GMA), methanol (MeOH), and other chemicals, such as potassium persulfate (KPS), polyvinyl alcohol (PVA), ethylene glycol dimethacrylate (EGDMA), imino diacetic acid (IDA), CuSO4, were purchased from Sigma-Aldrich company.
8
Determination of nanoFc pI by cIEF
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The pI of nanoFc was determined using Capillary Isoelectric Focusing (cIEF, CESI 8000 plus, AB Sciex, Sweden). To accomplish this, 10 μL of nanoFc (5 mg/mL) was mixed with 200 μL Urea‐cIEF gel (3 M, Sigma–Aldrich, USA), 12 μL ampholytes (3–10, Sigma–Aldrich, USA), 20 μL arginine (500 mM, Sigma–Aldrich, USA), 20 μL iminodiacetic acid (200 mM, Sigma–Aldrich, USA), and 2 μL pI marker (Sigma–Aldrich, USA), and subsequently loaded onto the system. The mixture was prefocused for 15 min at 25 kV, followed by a chemical mobilization at 30 kV for 30 min. The whole‐column UV detector at 280 nm was used to acquire the main component and charge variants after they were focused on their respective pI values.
9
Capillary isoelectric focusing (cIEF) protocol
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Sodium hydroxide, phosphoric acid, glacial acetic acid, urea, iminodiacetic acid, 2-mercaptoethanol and L-arginine were from Sigma Aldrich (St. Louis, MO, USA). The Pharmalyte 3–10 was from GE Healthcare (Chicago, IL, USA). The cIEF Gel; the pI markers of the pI Peptide Marker kit; the Fast Glycan kit; and the SDS-MW Analysis Assay kit including the SDS-MW Gel Buffer, Sample Buffer and 10 kDa protein standard along with the 50 µm ID NCHO and BFS capillaries were from Bio-Science Kft (Budapest, Hungary). The hIgG1 test sample was from Molecular Innovations (Novi, MI, USA). The commercial PNGase F enzyme was from New England Biolabs (Ipswich, MA, USA). The in-house produced 6His-PNGase F (production described in detail in [20 (link)] and this enzyme featured 3 months of shelf life) was from University of Pannonia (Veszprem, Hungary).
10
Biosensor Synthesis and Characterization
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l ‐Lysine hydrochloride, S‐methylbenzylamine, pyruvate, pyridoxal 5’‐phosphate monohydrate, ethylenediamine, 1,4‐butanediol diglycidyl ether, iminodiacetic acid, sodium borate, potassium phosphate, cobalt chloride, glucosamine, sodium periodate, l ‐thiazolidine‐4‐carboxilic acid, and fluoresceine isothiocyanate were purchased from Sigma Aldrich (Gillingham, U.K). Polyethyleneimine 50 % aq. solution branched 60000 Da was acquired from Thermo Fisher Scientific. Nicotinamide adenine dinucleotide reduced form (NADH) and oxidized form (NAD+) were purchased from Apollo Scientific. 6‐channel μ‐slide VI was purchased from ibidi (Planegg, Germany). All other reagents were of analytical grade unless otherwise specified.
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