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Tgfα elisa kit

Manufactured by Thermo Fisher Scientific
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The TGFα ELISA Kit is a quantitative sandwich enzyme immunoassay designed to measure the concentration of Transforming Growth Factor alpha (TGFα) in human, mouse, and rat samples. The kit utilizes a specific antibody coated on a microplate to capture TGFα from the samples, and a second antibody conjugated to an enzyme for detection. The resulting color reaction is proportional to the concentration of TGFα present in the sample.

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Lab products found in correlation

Th1 th2 elisa kit, by Thermo Fisher Scientific (2 mentions) Il 12p70 elisa kit, by Thermo Fisher Scientific (2 mentions) Egf elisa kit, by Thermo Fisher Scientific (2 mentions) Igf 1 elisa kit, by Thermo Fisher Scientific (2 mentions)

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ELISAs (35 citations)

2 protocols using tgfα elisa kit

1

Cytokine Profiling in Bladder Infection

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Bladders were collected at different time point post infection as indicated in the figure legends. Then bladders were homogenized by using zirconia silica beads for three cycles of 1.5 min each in an automatic homogenizer, and enzyme-linked immunosorbent assay was performed by using TH1/TH2 ELISA Kit (88–7711-44, ThermoFisher), IL-12p70 ELISA Kit (BMS6004, ThermoFisher), EGF ELISA Kit (EMEGF, ThermoFisher), IGF-1 ELISA Kit (EMIGF1, ThermoFisher), or TGFα ELISA Kit (EHTGFA, ThermoFisher) following manufacture’s instruction. Concentrations were determined by standard curve. The level of IFN-γ and IL-4 at various time points was normalized to the level at day 0 without infection.
Wu J., Hayes B.W., Phoenix C., Macias G.S., Miao Y., Choi H.W., Hughes FM J.r., Purves J.T., Reinhardt R.L, & Abraham S.N. (2020). A Highly Polarized Th2 Bladder Response to Infection Promotes Epithelial Repair at the Expense of Preventing New Infections. Nature immunology, 21(6), 671-683.
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2

Cytokine Profiling in Bladder Infection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Bladders were collected at different time point post infection as indicated in the figure legends. Then bladders were homogenized by using zirconia silica beads for three cycles of 1.5 min each in an automatic homogenizer, and enzyme-linked immunosorbent assay was performed by using TH1/TH2 ELISA Kit (88–7711-44, ThermoFisher), IL-12p70 ELISA Kit (BMS6004, ThermoFisher), EGF ELISA Kit (EMEGF, ThermoFisher), IGF-1 ELISA Kit (EMIGF1, ThermoFisher), or TGFα ELISA Kit (EHTGFA, ThermoFisher) following manufacture’s instruction. Concentrations were determined by standard curve. The level of IFN-γ and IL-4 at various time points was normalized to the level at day 0 without infection.
Wu J., Hayes B.W., Phoenix C., Macias G.S., Miao Y., Choi H.W., Hughes FM J.r., Purves J.T., Reinhardt R.L, & Abraham S.N. (2020). A Highly Polarized Th2 Bladder Response to Infection Promotes Epithelial Repair at the Expense of Preventing New Infections. Nature immunology, 21(6), 671-683.
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