Fb25015

HK-2 (human kidney-2, a proximal tubular cell line) and breast cancer cell line MDA-MB231 were obtained from the American Type Culture Collection (Manassas, VA, USA). The HK-2 cells were maintained in RPMI-1640 medium (10-040-CV, Corning, CA, USA) containing 10% fetal bovine serum and 1% penicillin/streptomycin. The MDA-MB231 cells were cultured in DMEM medium without L-glutamine (15-017-CVR, Corning, CA, USA), supplemented with 10% fetal bovine serum (FB25015, Clark Bioscience, Richmond, VA, USA), 1% GlutaMAX (35050-061, Gibco, Carlsbad, CA, USA), and 1% penicillin/streptomycin (SV30010, HyClone, Logan, UT, USA). All cells were maintained in a cell incubator (Thermo, Waltham, MA, USA) at 37 °C and 5% CO₂.

Crandell-Rees feline kidney (CRFK) cells were available in our laboratory. Baby Hamster Kidney cell clone (BSR-T7/5), which stably expresses T7 RNA polymerase (T7 pol), was kindly provided by Zhigao Bu (Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences). Felis catus whole-fetus 4 (FCWF-4) cells were purchased from the ATCC. All cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Sigma Aldrich, D6429) with 10% FBS (Clark, FB25015) at 37°C in an atmosphere of humidified air containing 5% (v/v) CO₂.

We tested 19 cell lines in this study, which are all adherent cells. The GIST-T1 cell line (gastrointestinal stromal tumor) was from Cosmo Bio Co. Ltd. (Tokyo, Japan). All other cell lines were from American Type Culture Collection (Manassas, VA, USA).
MCF-7, MDA-MB-231, GIST-T1, HeLa (cervix epithelial adenocarcinoma), HCT116 (colon epithelial carcinoma), PC3 (prostate adenocarcinoma), C6 (rat brain glial), HepG2 (hepatocellular carcinoma), RPE1 (retina epithelial), 293T (kidney epithelial), differentiated PC-12 (rat pheochromocytoma), NIH-3T3 (mouse embryo fibroblast), and CHO (Chinese hamster ovary) cells were cultured in DMEM medium without L-glutamine (15-017-CVR, Corning, NY, USA), supplemented with 10% (v/v) FBS (fetal bovine serum) (FB25015, Clark Bioscience, Richmond, VA, USA), 1% GlutaMAX (35050-061, Gibco, Carlsbad, CA, USA), and 1% (v/v) P/S (penicillin/streptomycin) (SV30010, HyClone, Logan, UT, USA). CNE-2Z (nasopharyngeal cancer), EJ1 (bladder cancer), and U251 (brain glioblastoma) cells were cultured in RPMI 1640 without L-glutamine (15-040-CVR, Corning) and supplemented with 10% FBS, 2 mM GlutaMAX, and 1% P/S. Three human noncancer lung cells (HSAEC2-KT, HSAEC30-KT, and HBEC30-KT) were cultured in SAGM (Lonza, CC-3118). All cells were maintained in a cell incubator (BC-J160S, Shanghai Boxun, Shanghai, China) at 37.0°C and 5% CO₂.

The cell lines MDA-MB231 (RRID: CVCL_0062) and MCF-7 (RRID: CVCL_0031) were from American Type Culture Collection. Cell line identity was confirmed and cultured in Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (CLARK Bioscience, FB25015).

Human normal gastric epithelial cell line GES-1 and GC cell lines MGC803, SGC7901, HGC27, BGC823 and AGS were obtained from the Chinese Academy of Sciences (Shanghai, China). All cell lines were maintained in (RPMI)-1640 medium (R10-040-CV, Corning, USA), added with 10% FBS (FB25015, CLARK, USA) and 1% Penicillin–Streptomycin Solution (SV30010, HyClone, USA). All cell lines were maintained at 37 °C in an incubator containing a humidified 5% CO₂ atmosphere.

The murine astrocyte cell line C8-D1A was commercially obtained from Fuheng (FH0837, FuHeng Cell Center, Shanghai, China). Human GM U373, HS683 and U138MG cell lines were commercially obtained from ATCC. SHG44, U251, T98G, TJ905 and A172 cells were commercially obtained from Bolida (Bolida, Xuzhou, Jiangsu, China). The cells were cultured in DMEM containing 10% heat-inactivated FBS (FB25015, Clark, Virginia, USA), 100 mg/L streptomycin and 100 U/mL penicillin at 37 °C in a 5% CO₂ environment. All human GM cell lines have been authenticated using STR profiling within the last three years. In addition, all experiments were performed with mycoplasma-free cells.