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Hyclone dmem high glucose medium

Manufactured by Thermo Fisher Scientific
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HyClone® DMEM high glucose medium is a cell culture medium used for the growth and maintenance of various cell lines. It provides a high concentration of glucose to support cellular metabolism and growth. The formulation is designed to maintain an optimal pH and osmolarity for cell culture applications.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (6 mentions) Dmem high glucose medium, by Keygen Biotech (3 mentions) Penicillin and streptomycin, by Thermo Fisher Scientific (2 mentions) Co2 incubator, by Thermo Fisher Scientific (1 mentions) Gibco 10 fetal bovine serum fbs, by Thermo Fisher Scientific (1 mentions) Falcon 70 μm cell strainer, by Corning (1 mentions) Percoll solution, by GE Healthcare (1 mentions) Red blood cell lysis buffer, by Merck Group (1 mentions) Lipofectamine 3000 reagent, by Promega (1 mentions) Prl tk, by Promega (1 mentions) Fetal bovine serum (fbs), by Keygen Biotech (1 mentions)

Close spelling variants of this product

Hyclone Dulbecco’s modified Eagle’s medium (DMEM)/high glucose Hyclone high glucose DMEM DMEM high glucose medium HyClone™ DMEM medium High-glucose DMEM High glucose medium HyClone® DMEM DMEM-glucose High glucose High DMEM

7 protocols using hyclone dmem high glucose medium

1

Primary CRC cell cultivation

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After obtaining written informed consent, primary cells were isolated from 12 CRC patients treated at Shanghai Municipal Hospital of Traditional Chinese Medicine Affiliated to Shanghai TCM University (Shanghai, China). The cells were cultured in a 5% CO2 incubator (Thermo Fisher Scientific, Inc., Waltham, MA, USA) at 37°C with HyClone™ high glucose DMEM medium (cat. no. SH30243.01; Thermo Fisher Scientific, Inc.) supplemented with Gibco™ 10% fetal bovine serum (FBS; cat. no. 16000-044; Thermo Fisher Scientific, Inc.) and 1% antibiotic (penicillin and streptomycin; cat. no. P1400-100; Beijing Solarbio Science & Technology Co., Ltd., Beijing, China). The medium was refreshed every two days during culture.
Tong J., Shen Y., Chen X., Wang R., Hu Y., Zhang X., Zhang Z, & Han L. (2019). FKBP3 mediates oxaliplatin resistance in colorectal cancer cells by regulating HDAC2 expression. Oncology Reports, 42(4), 1404-1412.
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2

Isolation of Murine Liver Mononuclear Cells

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To obtain MNCs, livers were harvested and pressed through a Falcon® 70μm cell strainer (Corning, NY), and suspended in HyClone™ High Glucose DMEM Medium (Thermo Fisher Scientific, Whaltham, MA). Liver MNCs were washed and the cell pellet was resuspended in 35% Percoll solution (GE Healthcare Life Sciences, Marlborough, MA) containing 100 U/ml of heparin and centrifuged at 2000 rpm at room temperature for 15 min. The supernatant was carefully removed and erythrocytes were disrupted by Red Blood Cell Lysis Buffer (Millipore Sigma, Burlington, MA). The cells were washed with DMEM supplemented with 2% of FBS and isolated liver MNCs cells were counted with a hemocytometer using trypan blue and resuspended in FACS buffer (0.02% sodium azide, 2% FBS in PBS).
Maricic I., Marrero I., Eguchi A., Nakamura R., Johnson C.D., Dasgupta S., Hernandez C.D., Nguyen P.S., Swafford A.D., Knight R., Feldstein A.E., Loomba R, & Kumar V. (2018). Differential activation of hepatic iNKT cell subsets plays a key role in progression of nonalcoholic steatohepatitis. Journal of immunology (Baltimore, Md. : 1950), 201(10), 3017-3035.
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3

Luciferase activity assay in HEK-293 and SK-N-SH cells

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Human embryonic kidney cell line HEK-293 and neuroblastoma cell line SK-N-SH were used to test luciferase activity of the pGL-3 recombinant vectors. HEK-293 cells were cultured in HyClone® DMEM high glucose medium with 10% fetal bovine serum (Thermo Fisher Scientific, Massachusetts, USA). SK-N-SH cells were cultured in KeyGRN BioTECH® DMEM high glucose medium (with 0.011 g/L sodium pyruvate) with 15% fetal bovine serum. Cells were seeded in 24-well plates (2 × 105 cells per well). According to the manufacturer’s protocol (Invitrogen, California, USA), Lipofectamine®3000 reagent was used to co-transfect the pGL-3 recombinant plasmids containing the two haplotypes with the Renilla luciferase-expressing vector pRL-TK (Promega) into the two cell lines. Cells were harvested after 24 h in culture. Firefly luciferase activity (LUC value) was measured and normalized to renilla luciferase activity (TK value). Each assay was performed in triplicate in the two cell lines.
Liu Y.P., Ding M., Zhang X.C., Liu Y., Xuan J.F., Xing J.X., Xia X., Yao J, & Wang B.J. (2019). Association between polymorphisms in the GRIN1 gene 5′ regulatory region and schizophrenia in a northern Han Chinese population and haplotype effects on protein expression in vitro. BMC Medical Genetics, 20, 26.
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4

Transfection of Recombinant Vectors in HEK-293 and SK-N-SH Cells

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A total of 14 recombinant vectors were transfected into human embryonic kidney cell HEK-293 and human neuroblastoma cell SK-N-SH lines (Human embryonic kidney cell HEK-293 and human neuroblastoma cell SK-N-SH were purchased from the Chinese Academy of Sciences cell bank and catalog numbers were GNHu43 and TCHu51, respectively.). HEK-293 cells were incubated in HyClone® DMEM high-glucose medium containing 10% fetal bovine serum (Thermo Fisher Scientific, Waltham, Massachusetts, USA); they were maintained in 5% CO2 + 95% mixed air at 37 °C. SK-N-SH cells were treated with KeyGEN BioTECH® DMEM high-glucose medium with 0.110 g/L sodium pyruvate containing 15% fetal bovine serum under the same conditions. Transfection experiments were performed when the cell density reached 90% or more [17 (link)].
Wu X., Xu F.L., Ding M., Zhang J.J., Yao J, & Wang B.J. (2018). Characterization and functional analyses of the human HTR1A gene: 5’ regulatory region modulates gene expression in vitro. BMC Genetics, 19, 115.
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5

Culturing Human Cell Lines

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Human embryonic kidney HEK-293 cells were cultured in HyClone® DMEM high glucose medium containing 10% fetal bovine serum (Thermo Fisher Scientific, Waltham MA, USA), 5% CO2 + 95% mixed air at 37 °C. Neuroblastoma SK-N-SH cells were treated with KeyGEN BioTECH® DMEM high glucose medium with 0.110 g/L sodium pyruvate containing 15% fetal bovine serum under the same conditions.
Liu Y.P., Wu X., Meng J.H., Ding M., Xu F.L., Zhang J.J., Yao J, & Wang B.J. (2019). Transcription Factor CEBPB Inhibits the Expression of the Human HTR1A by Binding to 5′ Regulatory Region In Vitro. Genes, 10(10), 802.
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6

Transfection of Recombinant Vectors in Cell Lines

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Transfection of recombinant vectors was conducted with four cell lines: HEK-293, U87, SK-N-SH and SH-SY5Y. HEK-293 and U87 cells were cultured in HyClone® DMEM high glucose medium containing 10% fetal bovine serum (Thermo Fisher Scientific, Chelmsford, MA, USA), and the SK-N-SH cell line was treated with KeyGEN BioTECH ® DMEM high glucose medium with 0.110 g/L sodium pyruvate containing 15% fetal bovine serum. SH-SY5Y cells were cultured in Corning® DMEM/F12 1:1 mix medium +15% fetal bovine serum. All cells were cultured to a density of more than 90% at 37°C in 5% CO2 + 95% mixed air.
Wu X., Ding M., Liu Y., Xia X., Xu F.L., Yao J, & Wang B.J. (2019). hsa-miR-3177-5p and hsa-miR-3178 Inhibit 5-HT1A Expression by Binding the 3′-UTR Region in vitro. Frontiers in Molecular Neuroscience, 12, 13.
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7

Transfection of HEK-293, SK-N-SH, and SH-SY5Y cells

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Human embryonic kidney cell line HEK‐293 and human neuroblastoma cell lines SK‐N‐SH and SH‐SY5Y were transfected with recombinant vectors. HEK‐293 cells were cultured in HyClone® DMEM high glucose medium containing 10% fetal bovine serum (Thermo Fisher Scientific, MA), while SK‐N‐SH cells were cultured in KeyGEN BioTECH® DMEM high glucose medium with 0.011 g/L sodium pyruvate containing 15% fetal bovine serum. SH‐SY5Y cells were cultured in HyClone® DMEM/F‐12 mixed medium. Cell transfection was performed when cell density reached 90%.
Liu Y., Ding M., Liu Y., Zhang X., Xing J., Xuan J., Xia X., Yao J, & Wang B. (2019). Functional analysis of haplotypes and promoter activity at the 5′ region of the human GABRB3 gene and associations with schizophrenia. Molecular Genetics & Genomic Medicine, 7(5), e652.
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