Ten specimens (including five left and five right femur specimens) from each group were scanned by micro-CT (Siemens, Munich, Germany) at a scanning rate of 6°/min and a resolution of 9 μm. The X-ray source voltage was 80 kV, and the beam current was 80 mA using filtered Bremsstrahlung radiation. A 1-mm aluminium filter was used during the scanning. The micro-CT images were then reconstructed using multimodal 3D visualisation software (Inveon Research Workplace; Siemens, Munich, Germany). The bone was distinguished from soft tissue and titanium implants by partitioning different Hounsfield units (HUs). The phase of the bone was defined in the range of 1000–2250 HU. After the reconstruction, the peripheral 500-μm region around and the intra-porous space within the implant were selected as the region of interest (ROI). In the ROI, the bone volume/tissue volume (BV/TV, the ratio of bone volume to total volume) and trabecular separation (Tb.Sp, mean width of the medullary cavity between bone trabeculae) were calculated using Inveon Research Workplace software (Siemens, Munich, Germany).
Micro ct
Manufactured by Siemens
Sourced in Germany, United States
Micro-CT is a non-destructive imaging technique that uses X-rays to produce high-resolution, three-dimensional (3D) images of small samples. It provides detailed information about the internal structure and composition of the sample, allowing for precise analysis and measurement.
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Lab products found in correlation
Inveon research workplace, by Siemens (6 mentions)
Mimics 19, by Materialise (2 mentions)
Skyscan 1174 micro ct system, by Bruker (2 mentions)
Dhf 155hii, by Hitachi (2 mentions)
Inveon acquisition workplace, by Siemens (2 mentions)
Inveon research workplace software, by Siemens (1 mentions)
Micro ct imaging system, by Siemens (1 mentions)
Sodium borohydride solution, by Sinopharm (1 mentions)
High speed centrifuge, by Thermo Fisher Scientific (1 mentions)
Inveon analysis workstation, by Siemens (1 mentions)
Microview, by GE Healthcare (1 mentions)
Microplate reader, by Thermo Fisher Scientific (1 mentions)
Inveon research workplace irw, by Siemens (1 mentions)
Microfil mv 122, by Flow Tech (1 mentions)
7900ht fast real time pcr system, by Thermo Fisher Scientific (1 mentions)
Inveon software, by Siemens (1 mentions)
42 protocols using micro ct
1
Micro-CT Analysis of Bone-Implant Interface
2
Femoral Microarchitecture Analysis via Micro-CT
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To evaluate skeletal microarchitecture, the right femora (n = 6) were subjected to a high-resolution Micro-CT analysis using a Micro-CT imaging system (Siemens, Inveon), according to the method described in a publication [41 (link)].
The quantitative parameters of the bone microstructure were measured, including BV/TV, BS/BV, Tb.Th, Tb.N, Tb.Sp and BMD. All the digitalized data and 3-dimensional images were supplied by the built-in software of Micro-CT (Siemens, Inveon).
The quantitative parameters of the bone microstructure were measured, including BV/TV, BS/BV, Tb.Th, Tb.N, Tb.Sp and BMD. All the digitalized data and 3-dimensional images were supplied by the built-in software of Micro-CT (Siemens, Inveon).
3
Micro-CT Analysis of Mouse Knee Joints
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At the end of week 12, all mice were anesthetized with sodium pentobarbital intraperitoneally (40 mg/kg) and then sacrificed by cervical dislocation. The knee joints of all groups were dissected and fixed in 4% paraformaldehyde. The mouse samples were scanned with Micro‐CT (Siemens Co., Knoxville, Tennessee, USA), which was performed at the following parameters: voltage of 70 kV, an electric current of 114 μA, and resolution of 10 μm per pixel. Three‐dimensional structures were analyzed, including bone volume (BV), bone volume/total tissue volume (BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th), and trabecular separation (Tb.Sp).
4
ApoE Knockout Mice Nanoparticle Study
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Thirty 6-week-old male ApoE knockout mice (ApoE-/-) without specific pathogens were provided by Beijing Huafukang Biotechnology Co., Ltd. (body weight 20.0 ± 0.5 g). Cetrimonium Bromide (CTAB) solution was provided by Dingguo Biotechnology Co., Ltd. The chloroauric acid solution was provided by the American Sigma Company. The sodium borohydride solution, sodium chloride solution, and chloroauric acid solution were all provided by Sinopharm Chemical Reagent Co., Ltd. Ascorbic acid was provided by Shanghai Macleans Biochemical Co., Ltd. Polyethylene glycol was provided by Ma Yinglong Pharmaceutical Group Co., Ltd. Micro-CT is provided by Siemens, Germany. The high-speed centrifuge is provided by Thermo Fisher Scientific Co., Ltd. Transmission electron microscopy and laser particle size analyzer were provided by Delong Company of the United States and Malvern Company of the United Kingdom, respectively.
5
Micro-CT Analysis of Rat TMJ Bone
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All Sprague–Dawley rats underwent general anesthesia at 1, 4, and 8 weeks postoperatively. Their skulls were scanned by Micro-CT (Siemens AG) at a voltage of 80 kV, a current of 500 μA, and a resolution of 10 μm. Subsequently, three-dimensional (3D) images of TMJ complexes were reconstructed with the Inveon Research Workplace (Siemens AG). In the operation group, the region of interest (ROI) included three cubes (each 0.5 × 0.5 × 0.5 mm), which were selected along an imaginary line between the concave point of the condylar lateral neck and the upper edge of the zygomatic arch base. The morphological parameters of trabecular bone microarchitecture, including bone volume fraction (BV/TV), trabecular thickness (Tb.Th), bone specific surface (BS/BV), trabecular separation (Tb.Sp) and trabecular number (Tb.N), were measured.
6
Evaluating Bone Formation in Cranium
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Excised samples in the cranium were scanned with micro-CT (Siemens Inveon, Germany) to evaluate new bone formation within the implant sites. The scan was at energy of 80 kV and 500 μA, a current of 145 mA, and an isotropic voxel size of 27 μm. After 3D reconstruction, quantitative analyses of bone volume/total volume (BV/TV), trabecular thickness (Tb.Th), trabecular separation/spacing (Tb.Sp) and trabecular number (Tb.N) in the same region of interest within defects were performed using Inveon Research Workplace 2.2 [46 (link)].
7
Femoral Bone Histomorphometry and Micro-CT Analysis
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The femoral specimens were incubated with 4% paraformaldehyde for 4 days for tissue fixation and with 10% tetracycline-EDTA for 3 weeks for decalcification. The distal femur was cut into 4 mm sections for H&E and TRAP staining. Scanning HE stained sections with Aperio Scanscope, histomorphometric parameters were recorded and analyzed. TRAP staining was utilized to determine the number and morphology of osteoclasts in each section. Micro-CT (Siemens, Germany) was employed to scan 100 slices of each bone growth plate. The image data of bones and trabecular bone were analyzed by Mimics 15.0 software (Matralise, Belgium).
8
Maxillae Micro-CT Imaging for OTM
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Freshly dissected maxillae were collected and scanned by Micro‐CT (Siemens Inveon, Germany). The sagittal and horizontal images were acquired through three‐dimensional reconstructions. OTM distance was measured as previously described.17
9
Micro-CT Analysis of Bone Formation
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The rats were sacrificed at 4th, 8th, and 12th week postoperatively, and the femoral condyles were removed to evaluate new bone formation in the scaffold at the defect using high-resolution Micro-CT (Siemens, Munich, Germany). Scanning was performed with resolution 20 μm, voltage 60 kV, and current 400 μA. The mean CT values of new bone and scaffold analyzed by INVEON Workplace software were 3590HU and 1890HU, respectively. Region of interest (ROI) was selected according to the anatomical location of the bone defect. First, the CT value of the new bone tissue was used to identify and select the new bone tissue in the ROI. Then, the scaffold in the ROI was identified and selected by using the CT value of the scaffold. By removing the selected portion of the scaffold from the selected portion of the newly formed bone tissue, the actual portion of the newly formed bone tissue in the scaffold could be obtained. Statistical analysis was performed on the bone tissue volume/total tissue volume (BV/TV), trabecular number (Tb·N), trabecular separation/spacing (Tb.Sp) of new bone tissue in each group.
10
Rabbit Jawbone Regeneration Analysis
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Three months after the model was established, the rabbits were sacrificed, and the upper and lower jawbones were obtained for examination by micro-CT (80 kV, 2 s, Siemens Inveon, Munich, Germany). CTAn software was used to reconstruct and calculate the volume of regenerated tissue in the blank control, DPSCs and DPSCs/PDL groups.
After rabbit jawbones from the blank control, DPSCs and DPSCs/PDL groups were cut on the coronal plane, a stereomicroscope was used for general observation of the regenerated tissue, and SEM (Phenom-World Co., Ltd., Netherlands) was used to observe the microstructure.
After rabbit jawbones from the blank control, DPSCs and DPSCs/PDL groups were cut on the coronal plane, a stereomicroscope was used for general observation of the regenerated tissue, and SEM (Phenom-World Co., Ltd., Netherlands) was used to observe the microstructure.
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