Miniphoto 518 r spectrophotometer

Manufactured by TAITEC

Sourced in Japan

The Miniphoto 518 R is a spectrophotometer designed for accurate and reliable absorbance measurements across a wide range of applications. It features a compact design, intuitive user interface, and advanced optics for efficient data collection and analysis.

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Lab products found in correlation

F kit d glucose, by Roche (2 mentions) Protein assay kit, by Bio-Rad (1 mentions) Myo inositol assay kit, by Megazyme (1 mentions)

5 protocols using miniphoto 518 r spectrophotometer

Yeast Growth Conditions and Harvesting

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Saccharomyces cerevisiae BY418 (MATα ade2Δ::hisG his3Δ200 leu2Δ1 lys2Δ202 met15Δ0 trp1Δ63 ura3-52) was grown at 30°C in liquid media YPD (1.0% [w/v] yeast extract, 2.0% [w/v] polypeptone, and 2.0% [w/v] d-glucose), YPGly (1.0% [w/v] yeast extract, 2.0% [w/v] polypeptone, and 2.0% [w/v] glycerol), or SD (0.67% [w/v] yeast nitrogen base without amino acids and 2.0% [w/v] d-glucose) with 20 µg/mL appropriate amino acid and nucleobase supplements. Cell growth was monitored by measuring OD₆₆₀ using a Miniphoto518R spectrophotometer (Taitec). The cells were harvested at the log phase (OD₆₆₀ ≈ 0.3) or at the stationary phase by centrifugation at 1610g for 3 min. The cell pellets were then frozen quickly in liquid nitrogen.

Nagai A., Mori K., Shiomi Y, & Yoshihisa T. (2021). OTTER, a new method for quantifying absolute amounts of tRNAs. RNA, 27(5), 628-640.

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Bacterial Growth and Metabolite Measurement

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Bacterial growth was monitored by measuring the optical density at 660 nm (OD₆₆₀) of the culture broth using a Miniphoto 518 R spectrophotometer (Taitec, Saitama, Japan). Concentrations of glucose, myo-inositol, and protein were determined using their respective assay kits, as described previously (50 (link)).

Ikeda M., Takahashi K., Ohtake T., Imoto R., Kawakami H., Hayashi M, & Takeno S. (2021). A Futile Metabolic Cycle of Fatty Acyl Coenzyme A (Acyl-CoA) Hydrolysis and Resynthesis in Corynebacterium glutamicum and Its Disruption Leading to Fatty Acid Production. Applied and Environmental Microbiology, 87(4), e02469-20.

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Bacterial Cultivation Protocols for E. coli

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E. coli strains and plasmids used in this study are shown in Table S1. E. coli cells were grown at 37°C in Luria-Bertani (LB) medium. Cell growth was monitored by measuring the turbidity with a Mini photo 518R spectrophotometer (Taitec). The standard procedure for bacterial cell cultivation in this study was as follows: A single colony was isolated from an overnight culture on a LB agar plate, and inoculated into 5 ml of fresh LB medium. This liquid culture was grown overnight at 37°C, and the overnight culture was diluted 100-fold into fresh LB medium. The culture was incubated at 37°C with reciprocal shaking (160 revolutions min⁻¹) for aerobiosis or without shaking for anaerobiosis.

Yamanaka Y., Oshima T., Ishihama A, & Yamamoto K. (2014). Characterization of the YdeO Regulon in Escherichia coli. PLoS ONE, 9(11), e111962.

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Microbial Growth and Metabolite Quantification

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Bacterial growth was monitored by measuring the optical density at 660 nm (OD 660 ) of the culture broth with a Miniphoto 518R spectrophotometer (Taitec, Saitama, Japan). Glucose concentration was determined using an F kit D-glucose (Roche Diagnostics, Basel, Switzerland) . Myo-inositol concentration was determined using a Myo-inositol assay kit (Megazyme International Ireland, Wicklow, Ireland). L-Lysine titer was determined as L-lysine HCl, according to the method described by Ohnishi et al. (2005) . Protein content was determined using a Bio-Rad protein assay kit (Bio-Rad Laboratories).

Takeno S., Hori K., Ohtani S., Mimura A., Mitsuhashi S, & Ikeda M. (2016). l-Lysine production independent of the oxidative pentose phosphate pathway by Corynebacterium glutamicum with the Streptococcus mutans gapN gene. Metabolic engineering, 37.

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Microbial Growth Monitoring via Spectrophotometry

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Bacterial growth was monitored by measuring the optical density at 660 nm (OD660) of the culture broth with a Miniphoto 518 R spectrophotometer (Taitec, Saitama, Japan).
Glucose concentration was determined using an F kit D-glucose (Roche Diagnostics, Basel, Switzerland). Dry cell weight (DCW) per liter of C. glutamicum strains was determined using the following formula that was established in advance: DCW (g) per liter = OD660 × 0.5007 -0.4202.

Takeno S., Murata N., Kura M., Takasaki M., Hayashi M, & Ikeda M. (2018). The accD3 gene for mycolic acid biosynthesis as a target for improving fatty acid production by fatty acid-producing Corynebacterium glutamicum strains. Applied microbiology and biotechnology, 102(24).

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