Tg content detection kit

All mice were raised under standard laboratory conditions under a 12-h light/dark cycle. After acclimatization for 1 week, mice were randomly separated into three groups: a CT group (n = 8) fed a chow diet (320 kcal/100 g) for 20 weeks, an HFD group (n = 8) and an EMPA group (n = 8) both fed an HFD (54.05% fat, 529.8 kcal/100 g) for 12 weeks and then administered saline or EMPA (dose: 10 mg/kg/d), respectively, for another 8 weeks. Body weight and fat mass were measured. After 20 weeks, glucose homeostasis was evaluated by glucose and insulin tolerance tests. At the end of the study, 2% barbiturate sodium (40 mg/kg, ip) was injected into mice. The small intestine tissues were rapidly isolated and rinsed with normal saline, then transferred into sterile cryopreservation tubes. The small intestine contents were collected and also transferred into sterile cryopreservation tubes. All samples were rapidly frozen and stored at −80°C for transcriptomic, metagenomic and metabolomics analysis. Serum TGs (triglycerides) was measured using a TG content detection kit (Solarbio, China).