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Sureplex amplification system

Manufactured by Illumina
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The SurePlex Amplification System is a laboratory instrument designed for DNA amplification. It provides a controlled and efficient process for increasing the quantity of DNA samples, enabling further analysis and applications.

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Lab products found in correlation

Repli g single cell kit, by Qiagen (2 mentions) Innoscan 710, by Innopsys (1 mentions) 24sure v3 microarray, by Illumina (1 mentions) Fluorescence labelling system, by Illumina (1 mentions) Bluefuse multi analysis software, by Illumina (1 mentions) Qubit dsdna hs high sensitivity assay kit, by Thermo Fisher Scientific (1 mentions) Qubit fluorometer, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

SurePlex DNA Amplification System (12 citations) Sureplex (7 citations) SurePlex amplification (2 citations)

4 protocols using sureplex amplification system

1

Comprehensive Chromosome Screening of Embryos

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The material obtained from each biopsy was amplified using the SurePlex amplification system (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions. PGT was carried out by aCGH using the 24 Sure V3 microarray (Illumina, San Diego, CA, USA) using the protocol described by Fragouli [28 (link), 29 (link)]. The amplified DNA was fluorescently labeled (Fluorescence Labelling System, Illumina). The samples were co-precipitated, denatured, and analyzed by array hybridization (for 16 h). A laser scanner (InnoScan 710, Innopsys, Carbonne, France) was used to excite the fluorophores and read the hybridization images. Hybridization images were stored in TIFF format and analyzed by the BlueFuse Multi-Analysis software (Illumina), using the criteria and algorithms recommended by the manufacturer. With this approach, it was possible to determine the chromosome constitution of each embryo.
Ocampo A., Pedraza J., Ortiz G., Hernández-Pérez E., Porchia L, & López-Bayghen E. (2018). Assessment of Prostaglandin-Endoperoxide Synthase 2 and Versican gene expression profile from the cumulus cells: association with better in vitro fertilization outcomes. Journal of Ovarian Research, 11, 84.
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2

Single-Cell Copy Number Variation Analysis

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Example 11

In this example, the method provided herein is compared with some current single cell preparation methods.

FIG. 9A shows copy number analysis using REPLIg Single Cell (MDA) with Nexteral XT library preparation. The REPLI-g Single Cell Kit developed by QIAGEN is specially designed to amplify genomic DNA from single cells (1 to <1000 cells) or purified genomic DNA with genome coverage. The REPLI-g Single Cell Kit developed by QIAGEN uses Multiple Displacement Amplification (MDA) technology. See Spits et al., 2006, Whole-genome multiple displacement amplification from single cells, Nature protocols 1 (4): 1965-70. However, due to MDA introduced over-amplification bias, the copy number variation data is very noisy when derived from a single cell, three cells or five cells, as shown in FIG. 9A.

FIG. 9B shows copy number analysis using SurePlex (PicoPlex) with Nexteral XT library preparation. SurePlex Amplification System developed by Illumina, Inc (San Diego, Calif.) is a solution for the extraction and amplification of DNA from single or few single cells. As shown, SurePlex Amplification System significantly reduces noise compared with MDA.

FIG. 9C shows copy number analysis using a method (Nextera SC) provided herein. As shown, the noise is further reduced compared with using SurePlex Amplification System.

US10017759B2. Library preparation of tagged nucleic acid (2018-07-10). Illumina, Inc. [US]. Inventors: Fiona Kaper [US], Gordon Cann [US].
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3

Single-Cell Copy Number Variation Analysis

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Example 11

In this example, the method provided herein is compared with some current single cell preparation methods.

FIG. 9A shows copy number analysis using REPLIg Single Cell (MDA) with Nexteral XT library preparation. The REPLI-g Single Cell Kit developed by QIAGEN is specially designed to amplify genomic DNA from single cells (1 to <1000 cells) or purified genomic DNA with genome coverage. The REPLI-g Single Cell Kit developed by QIAGEN uses Multiple Displacement Amplification (MDA) technology. See Spits et al., 2006, Whole-genome multiple displacement amplification from single cells, Nature protocols 1 (4): 1965-70. However, due to MDA introduced over-amplification bias, the copy number variation data is very noisy when derived from a single cell, three cells or five cells, as shown in FIG. 9A.

FIG. 9B shows copy number analysis using SurePlex (PicoPlex) with Nexteral XT library preparation. SurePlex Amplification System developed by Illumina, Inc (San Diego, Calif.) is a solution for the extraction and amplification of DNA from single or few single cells. As shown, SurePlex Amplification System significantly reduces noise compared with MDA.

FIG. 9C shows copy number analysis using a method (Nextera SC) provided herein. As shown, the noise is further reduced compared with using SurePlex Amplification System.

US11085041B2. Library preparation of tagged nucleic acid (2021-08-10). Illumina, Inc. [US]. Inventors: Fiona Kaper [US], Gordon Cann [US].
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4

DNA extraction and amplification from TE biopsies

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The Sureplex amplification system (Sureplex; Illumina, San Diego, CA, United States) was used for DNA extraction and amplification in the TE biopsies. A master mix, including cell extraction buffer, extraction enzyme dilution buffer, and extraction enzyme, was used to lyse the cells. Next, the released DNAs were fragmented and initially pre-amplified using Sureplex pre-amplification cocktail, which included Sureplex pre-amplification buffer and pre-amplification enzyme. Finally, the pre-amplified products were amplified using a Sureplex amplification cocktail containing amplification buffer, amplification enzyme, and nuclease-free water. A Qubit dsDNA HS (high-sensitivity) Assay Kit (Qubit®; Life Technologies, Waltham, MA, United States) was used to quantify the concentration of 10X diluted whole-genome-amplified products using a Qubit fluorometer (Qubit®; Life Technologies). Following the suggestions of manufacturer (Illumina), the products were diluted to 0.2 ng/μl for the following library preparation.
Chuang T.H., Chang Y.P., Lee M.J., Wang H.L., Lai H.H, & Chen S.U. (2021). The Incidence of Mosaicism for Individual Chromosome in Human Blastocysts Is Correlated With Chromosome Length. Frontiers in Genetics, 11, 565348.
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