The hearts of 13 WT and 13 Akita mice were isolated and Langendorff‐perfused, as described earlier.21 In short, the excised heart was quickly cannulated and retrogradely perfused with HEPES‐buffered tyrode solution bubbled with 100% O2, and the preparation was maintained at 36±1°C. Volume‐conducted ECG in lead II configuration was recorded using the PowerLab digitizer and the animal BioAmp from AD Instruments. As we have done previously,21 PVG was stimulated via a bipolar electrode using high‐frequency monophasic pulses (200 Hz, 300 μs, and 400 μA; Stimulus Isolator, AD Instruments) applied in trains of durations 300, 500, 700, and 1200 ms.21 Intervals between trains were at least 2 minutes. Stimuli were subthreshold for atrial myocardial excitation. The P‐P intervals were measured before and after stimulation in the absence and presence of 100 μmol/L propranolol and/ or 10 μmol/L atropine in the perfusate.
Stimulus isolator
Manufactured by ADInstruments
Sourced in Australia
The Stimulus Isolator is a lab equipment device designed to deliver controlled electrical stimuli to biological samples or preparations. It provides a constant current or voltage output, ensuring consistent and reliable stimulation. The Stimulus Isolator is a versatile tool used in various experimental setups within the life sciences field.
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Lab products found in correlation
2 protocols using stimulus isolator
1
Autonomic Regulation of Cardiac Conduction
2
Calibrating Painful Electrical Stimulation
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Before the start of each condition, individual current levels were determined. Small electrical stimuli were applied to the dorsum of the left little finger (Stimulus Isolator; ADInstruments, Sydney, Australia). Trains of fifty 500-µs (frequency = 50 Hz, total time = 1 second) stimuli were applied. After each train of stimuli participants gave a verbal pain rating between 0 and 10 (0 = no pain; 10 = worst pain imaginable). The calibration procedure started from a very low current, for which the participants could barely feel the stimulation, then the current was gradually increased. The calibration process was halted when participants rated the intensity as 7. We then repeated the current 10 more times to see if it can consistently create a painful feeling of 7 for at least 8 times. If not, the current was increased again until it did elicit a painful rating of 7 for 8 od 10 stimuli. This method of pain calibration has been used in previous studies, 32,35,52 32. Lapate, R.C. • Lee, H. • Salomons, T.V. ...
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