Hippocampi obtained from wild type and homozygous transgenic tau45-230 mice (3 to 12 month-old) were homogenized in 2X Laemmli buffer and boiled for 10 min. Whole cell extracts were also prepared from 1 to 21 days in culture hippocampal neurons prepared from wild type and homozygous transgenic tau45-230 mice. Lysates were loaded and run on sodium dodecyl sulfate (SDS)-poly-acrylamide gels as previously described (Laemmli, 1970 (link)). The proteins were transferred onto Immobilon membranes (Millipore, Billerica, MA) and immunoblotted (Towbin et al, 1979 (link)). Immunodetection was performed using anti-α-tubulin (clone DM1A; 1:200,000; Sigma), anti-synaptophysin (p38 1:1,000; Santa Cruz Biotechnology), anti-NR1 and NR2A (1:50; Santa Cruz Biotechnology), anti-NR2B (1:50; BD Biosciences, San Jose, CA), anti-Class III β-tubulin (clone TuJ1, 1:1,000; R&B Systems), anti-GFP (1:1,000; Millipore), and anti-integrin β1 (clone M-106, 1:100 Santa Cruz Biotechnology) antibodies. Secondary antibodies conjugated to horseradish peroxidase (1:1,000; Promega, Madison, WI) were used followed by enhanced chemiluminescence for the detection of proteins (Yakunin and Hallenbeck, 1998 (link)). The ChemiDoc XRS system and Quantity One Software (Bio-Rad) were used to image and analyze immunoreactive bands.
Anti nr2b
Manufactured by BD
Sourced in United States
Anti-NR2B is a laboratory reagent that binds to the NR2B subunit of the NMDA receptor. It is used in research applications to study the structure and function of NMDA receptors.
Automatically generated - may contain errors
Lab products found in correlation
Immobilon membrane, by Merck Group (1 mentions)
Horseradish peroxidase, by Promega (1 mentions)
Anti gfp, by Merck Group (1 mentions)
Anti α tubulin clone dm1a, by Merck Group (1 mentions)
Quantity one software, by Bio-Rad (1 mentions)
Chemidoc xrs system, by Bio-Rad (1 mentions)
Anti vdac1, by Abcam (1 mentions)
Anti synaptotagmin, by BD (1 mentions)
Anti aβ antibody 82e1, by Tecan (1 mentions)
Anti flotillin 1, by BD (1 mentions)
Anti glur1, by Merck Group (1 mentions)
Anti α tubulin, by Fujifilm (1 mentions)
Anti cox 4, by Cell Signaling Technology (1 mentions)
2 protocols using anti nr2b
1
Tau Protein Expression Analysis in Mouse Hippocampi
2
Antibody Sourcing for Neurodegenerative Research
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Anti-synaptotagmin, anti-PSD95, and anti-flotillin-1 antibodies were purchased from BD Bioscience (Franklin Lakes, NJ, USA). Anti-actin (Chemicon/Millipore, Billerica, MA, USA), anti-COX IV (Cell Signaling, Danvers, MA, USA), anti-α-tubulin (Wako Pure Chemical Industries, Osaka, Japan), and anti-VDAC1 (Abcam, Cambridge, UK) antibodies were purchased from the indicated suppliers. Anti-APP cytoplasmic antibody (G369, from Dr. Gandy) to detect APP FL and CTF, 13 anti-BACE1 (3D5, from Dr.Vassar), 14 and anti-PS1 CTF (G1L3, from Dr. Tomita) 15 were obtained as gifts from the indicated sources. Mouse monoclonal anti-Aβ antibodies 6E10 (raised against human Aβ1-16) and 4G8 (raised against human Aβ17-24) were purchased from BioLegend (San Diego, CA, USA), and anti-Aβ antibody 82E1 (raised against human Aβ1-16) was purchased from IBL (Fujioka, Gunma, Japan). Anti-GluR1 (Merck Millipore, Darmstadt, Germany), anti-NR2A (Thermo Fisher Scientific, Waltham, MA, USA), and anti-NR2B (BD Biosciences) antibodies were purchased from the indicated suppliers.
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