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Rabbit anti phospho src

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Rabbit anti-phospho-Src is a primary antibody that detects endogenous levels of Src protein only when phosphorylated at specific tyrosine residues. It is used for the detection and analysis of phosphorylated Src in various applications, such as Western blotting.

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Lab products found in correlation

Rabbit anti src, by Cell Signaling Technology (4 mentions) Rabbit anti histone h3 at serine 10, by Cell Signaling Technology (2 mentions) Mouse or rabbit anti α sma, by Santa Cruz Biotechnology (2 mentions) Image pro plus software, by Media Cybernetics (2 mentions) Mouse anti p21, by BD (2 mentions) Rabbit anti phospho smad1 5, by Cell Signaling Technology (2 mentions) Rabbit anti actin, by Cell Signaling Technology (2 mentions) Rabbit anti parp, by Santa Cruz Biotechnology (2 mentions) Rabbit anti gdf6, by Novus Biologicals (2 mentions) Sheep anti cd99l2, by R&D Systems (2 mentions) Mouse anti gst ms 707 p0, by Lab Vision (2 mentions) Rabbit anti phospho cortactin, by Cell Signaling Technology (2 mentions) Rabbit anti phospho p130 cas, by Cell Signaling Technology (2 mentions) Rabbit anti fli1, by Abcam (2 mentions) Rabbit anti ha, by Cell Signaling Technology (2 mentions) Rabbit anti caspase 3, by Cell Signaling Technology (2 mentions) Rabbit anti gapdh, by Santa Cruz Biotechnology (2 mentions) Rabbit anti phospho stat3, by Cell Signaling Technology (2 mentions) Mouse anti flag, by Merck Group (2 mentions) Mouse anti gfp, by Santa Cruz Biotechnology (2 mentions)

6 protocols using rabbit anti phospho src

1

Quantitative Assessment of Renal Fibrosis

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Immunofluorescent staining was carried out according to the procedure described in our previous studies.30 (link) For assessment of renal fibrosis quantitatively, the collagen tissue area was measured after Masson trichrome staining using Image Pro-Plus Software (Media-Cybernetics, Silver Spring, MD) by drawing a line around the perimeter of the positive staining area, and calculating and graphing the average ratio to each microscopic field (400×). For immunofluorescent staining, rabbit anti-histone H3 at serine 10, rabbit anti-phospho-Src (Cell Signaling Technologies), and mouse or rabbit anti-α-SMA (Santa Cruz Biotechnology) antibodies were used. The DAPI (4′,6-diamidino-2-phenylindole) staining for detection of apoptosis was conducted according to the protocol provided by the manufacture (Life Technologies, Grand Island, NY).
Yan Y., Ma L., Zhou X., Ponnusamy M., Tang J., Zhuang M.A., Tolbert E., Bayliss G., Bai J, & Zhuang S. (2016). Src inhibition blocks renal interstitial fibroblast activation and ameliorates renal fibrosis. Kidney international, 89(1), 68-81.
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2

Antibody-Based Protein Expression Analysis

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The detailed protocol has been published[24 (link)]. Primary antibodies used were rabbit anti-phospho-AKT (Ser473), rabbit anti-AKT, rabbit anti-phospho-FAK (Tyr397), rabbit anti-FAK, rabbit anti-eIF2α, rabbit anti-phospho-MEK1/2 (Ser217/221), rabbit anti-MEK1/2, rabbit anti-phospho-p38-MAPK (Thr180/Tyr182), rabbit anti-p38-MAPK, rabbit anti-phospho-Src (Tyr416), rabbit anti-Src (1:1000, Cell Signaling), goat anti-phospho-eIF2α (Ser51), rabbit anti-MMP2 (1:1000, Abgent), mouse anti-MMP9 (1:500, Abgent), rabbit anti-tubulin (1:1000, BioLegend). Secondary antibodies used were goat anti-mouse, goat anti-rabbit or donkey anti-goat IgG HRP conjugates (1:5000, Santa Cruz Biotechnology), accordingly. Relative levels from three independent experiments were presented as mean ± SEM.
Marín-Ramos N.I., Thein T.Z., Cho H.Y., Swenson S.D., Wang W., Schönthal A.H., Chen T.C, & Hofman F.M. (2018). NEO212 Inhibits Migration and Invasion of Glioma Stem Cells. Molecular cancer therapeutics, 17(3), 625-637.
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3

Immunoblotting and Immunoprecipitation Protocols

Cited 1 time
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Immunoblotting was performed as described (Jayabal et al., 2017 (link)). Immunoprecipitation was performed as described (Shiio and Eisenman, 2003 (link)). The following antibodies were used: rabbit anti-FLI-1 (ab15289, Abcam); rabbit anti-GDF6 (Novus, NBP 1-91934); rabbit anti-caspase 3 (9665, Cell Signaling Technology); rabbit anti-PARP (sc-7150, Santa Cruz Biotechnology); mouse anti-CD99 (MS-1633, Lab Vision); sheep anti-CD99L2 (AF5185, R&D Systems); mouse anti-p21 (BD PharMingen, 556430); mouse anti-tubulin (Developmental Studies Hybridoma Bank); rabbit anti-GAPDH (sc-25778, Santa Cruz Biotechnology); rabbit anti-CSK (HPA028425, Atlas Antibodies); mouse anti-GST (MS-707-P0, Lab Vision); mouse anti-GFP (sc-9996, Santa Cruz Biotechnology); rabbit anti-actin (8457, Cell Signaling Technology); rabbit anti-Src (2123, Cell Signaling Technology); rabbit anti-phospho-Src (2101, Cell Signaling Technology); rabbit anti-phospho-Cortactin (4569, Cell Signaling Technology); rabbit anti-phospho-p130 Cas (4011, Cell Signaling Technology); rabbit anti-phospho-STAT3 (9145, Cell Signaling Technology); rabbit anti-phospho-Smad1/5 (9516, Cell Signaling Technology); rabbit anti-HA (3724, Cell Signaling Technology); and mouse anti-FLAG (F1804, Sigma-Aldrich).
Zhou F., Elzi D.J., Jayabal P., Ma X., Chiu Y.C., Chen Y., Blackman B., Weintraub S.T., Houghton P.J, & Shiio Y. (2020). GDF6-CD99 Signaling Regulates Src and Ewing Sarcoma Growth. Cell reports, 33(5), 108332.
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4

Immunoblotting and Immunoprecipitation Protocols

Cited 1 time
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Immunoblotting was performed as described (Jayabal et al., 2017 (link)). Immunoprecipitation was performed as described (Shiio and Eisenman, 2003 (link)). The following antibodies were used: rabbit anti-FLI-1 (ab15289, Abcam); rabbit anti-GDF6 (Novus, NBP 1-91934); rabbit anti-caspase 3 (9665, Cell Signaling Technology); rabbit anti-PARP (sc-7150, Santa Cruz Biotechnology); mouse anti-CD99 (MS-1633, Lab Vision); sheep anti-CD99L2 (AF5185, R&D Systems); mouse anti-p21 (BD PharMingen, 556430); mouse anti-tubulin (Developmental Studies Hybridoma Bank); rabbit anti-GAPDH (sc-25778, Santa Cruz Biotechnology); rabbit anti-CSK (HPA028425, Atlas Antibodies); mouse anti-GST (MS-707-P0, Lab Vision); mouse anti-GFP (sc-9996, Santa Cruz Biotechnology); rabbit anti-actin (8457, Cell Signaling Technology); rabbit anti-Src (2123, Cell Signaling Technology); rabbit anti-phospho-Src (2101, Cell Signaling Technology); rabbit anti-phospho-Cortactin (4569, Cell Signaling Technology); rabbit anti-phospho-p130 Cas (4011, Cell Signaling Technology); rabbit anti-phospho-STAT3 (9145, Cell Signaling Technology); rabbit anti-phospho-Smad1/5 (9516, Cell Signaling Technology); rabbit anti-HA (3724, Cell Signaling Technology); and mouse anti-FLAG (F1804, Sigma-Aldrich).
Zhou F., Elzi D.J., Jayabal P., Ma X., Chiu Y.C., Chen Y., Blackman B., Weintraub S.T., Houghton P.J, & Shiio Y. (2020). GDF6-CD99 Signaling Regulates Src and Ewing Sarcoma Growth. Cell reports, 33(5), 108332.
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5

Immunoblotting of WAVE, FAK, and Src Signaling

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The following antibodies were used: rabbit antibodies against WAVE2 (1:1,000, Cell Signaling Technology, catalog no. 3659), Integrin β1/ CD29 (GeneTex, catalog no. GTX128839), and DGCR8 (Abcam, catalog no. Ab191875); mouse antibodies against β-actin (1:5,000, Sigma), WAVE1 (1:200, Santa Cruz Biotechnology, catalog no. Sc-136120), and WAVE2 (1:1,000, Santa Cruz Biotechnology, catalog no. Sc-373889). Rabbit anti FAK (1:1,000, Invitrogen, catalog no. UB281522), rabbit anti-phospho-FAK (1:1,000, Abcam, catalog no. ab81298), rabbit anti Src (1:1,000, Cell Signaling Technology, catalog no. 2109S), rabbit anti phospho-Src (1:1,000, Cell Signaling Technology, catalog no. 6943S), goat horseradish peroxidase–conjugated anti-mouse IgG and goat horseradish peroxidase–conjugated anti-rabbit IgG were from Bio-Rad (1:2,000). ECL reagent was from Thermo Fisher Scientific. For immunoblotting, primary antibodies were made in 5% BSA and secondary antibodies were made in 5% non-fat dry milk (NFDM).
Rana P.S., Wang W., Markovic V., Szpendyk J., Chan E.R, & Sossey-Alaoui K. (2023). The WAVE2/miR-29/Integrin-β1 Oncogenic Signaling Axis Promotes Tumor Growth and Metastasis in Triple-negative Breast Cancer. Cancer Research Communications, 3(1), 160-174.
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6

Quantitative Assessment of Renal Fibrosis

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Immunofluorescent staining was carried out according to the procedure described in our previous studies.30 (link) For assessment of renal fibrosis quantitatively, the collagen tissue area was measured after Masson trichrome staining using Image Pro-Plus Software (Media-Cybernetics, Silver Spring, MD) by drawing a line around the perimeter of the positive staining area, and calculating and graphing the average ratio to each microscopic field (400×). For immunofluorescent staining, rabbit anti-histone H3 at serine 10, rabbit anti-phospho-Src (Cell Signaling Technologies), and mouse or rabbit anti-α-SMA (Santa Cruz Biotechnology) antibodies were used. The DAPI (4′,6-diamidino-2-phenylindole) staining for detection of apoptosis was conducted according to the protocol provided by the manufacture (Life Technologies, Grand Island, NY).
Yan Y., Ma L., Zhou X., Ponnusamy M., Tang J., Zhuang M.A., Tolbert E., Bayliss G., Bai J, & Zhuang S. (2016). Src inhibition blocks renal interstitial fibroblast activation and ameliorates renal fibrosis. Kidney international, 89(1), 68-81.
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