Log In Sign up
The largest database of trusted experimental protocols

App clone 22c11

Manufactured by Merck Group
Visit Supplier
Sourced in United States

APP (clone 22C11) is a lab equipment product used for the detection and analysis of the amyloid precursor protein (APP). It is a purified monoclonal antibody that specifically binds to the APP molecule. The core function of this product is to serve as a tool for researchers to study APP and its role in various biological processes.

Automatically generated - may contain errors

Lab products found in correlation

α synuclein clone 42, by BD (1 mentions) β tubulin clone tub2, by Merck Group (1 mentions) Th nb300 109, by Novus Biologicals (1 mentions) Neun abn78, by Merck Group (1 mentions) Glial fibrillary acidic protein (gfap), by Agilent Technologies (1 mentions) Ab57632, by Abcam (1 mentions) Ki 67 clone d3b5, by Cell Signaling Technology (1 mentions) Glyceraldehyde 3 phosphate dehydrogenase, by Merck Group (1 mentions) Phospho tau at8, by Thermo Fisher Scientific (1 mentions) βiii tubulin, by BioLegend (1 mentions) Vglut1, by Synaptic Systems (1 mentions) Nf200, by Merck Group (1 mentions) βiii tubulin, by Abcam (1 mentions) Alexa conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions) Biotin conjugated secondary antibody, by Vector Laboratories (1 mentions) Olig2, by Merck Group (1 mentions) Cd11b, by Bio-Rad (1 mentions) β actin, by Merck Group (1 mentions)

Spelling variants of this product

APP (22C11) (4 citations) Anti-APP (clone 22C11; (4 citations) Mouse anti-APP N-terminal (clone 22C11; (2 citations) Mouse antibodies against APP (clone 22C11 (2 citations)

4 protocols using app clone 22c11

1

Plasmid Handling and Antibody Validation

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Human V5-tagged VPS35 plasmids were obtained from Addgene (no. 21691; ref. 58 (link)) and previously described (18 (link)). Mouse VPS35 cDNA was obtained from Dharmacon. Primary antibodies used were VPS35 (ab57632 or ab154647; Abcam), VPS26 (ab23892; Abcam), VPS29 (ab10160; Abcam), WASH1 (Sigma), Fam21A-D (S-13; Santa Cruz), V5 and V5-HRP (Life Technologies), TH (NB300-109; Novus Biologicals or clone TH-2; Sigma), α-synuclein (clone 42; BD Biosciences), pSer129-α-synuclein (clone EP1536Y; Abcam), APP (clone 22C11; Millipore), tau (clone Tau5; Millipore), phospho-tau (clones AT8, PHF1, CP13, and MC1, provided by Peter Davies, Feinstein Institute for Medical Research, New York), NeuN (ABN78; Millipore), Iba1 (Wako), GFAP (DAKO), and β-tubulin (clone TUB 2.1; Sigma). Secondary HRP-conjugated IgG, light chain-specific (Jackson Immunoresearch), biotinylated IgG (Vector Labs), and AlexaFluor-488 or -594 IgG (Thermo Fisher) were used.
Chen X., Kordich J.K., Williams E.T., Levine N., Cole-Strauss A., Marshall L., Labrie V., Ma J., Lipton J.W, & Moore D.J. (2019). Parkinson’s disease-linked D620N VPS35 knockin mice manifest tau neuropathology and dopaminergic neurodegeneration. Proceedings of the National Academy of Sciences of the United States of America, 116(12), 5765-5774.
+ Open protocol
+ Expand
2

Histological Analysis of Neurological Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mice were transcardially perfused with PBS containing 5 IU/ml heparin (De Pannemaeker, Ghent, Belgium), followed by perfusion with 4% paraformaldehyde. Brain and spinal cord tissue were dissected, dehydrated, and embedded in paraffin blocks. Alternatively, for vibratome sections brains were stored in low-melting agarose until further processing. Sections of 5 µm were stained with Luxol fast blue (Solvent Blue 38, practical grade; Sigma Genosys) for assessment of demyelination, or incubated with antibodies against CD3 (clone CD3–12; Serotex), Mac-3 (clone CD107b, M3/84; Becton Dickinson Biosciences), B220 (clone RA3-6B2; Becton Dickinson Biosciences), amyloid precursor protein (APP; clone 22C11; Millipore), Iba-1 (Wako Chemicals), or Ki67 (clone D3B5; Cell Signaling). Sections were rehydrated and incubated in antigen retrieval buffer (Dakopatts). Endogenous peroxidase activity was blocked by immersing slides in 3% H2O2. Nonspecific binding was blocked by incubating sections in 5% NGS and 0.1% Triton X-100. Primary antibodies were incubated overnight at 4 °C.
Voet S., Mc Guire C., Hagemeyer N., Martens A., Schroeder A., Wieghofer P., Daems C., Staszewski O., Vande Walle L., Jordao M.J., Sze M., Vikkula H.K., Demeestere D., Van Imschoot G., Scott C.L., Hoste E., Gonçalves A., Guilliams M., Lippens S., Libert C., Vandenbroucke R.E., Kim K.W., Jung S., Callaerts-Vegh Z., Callaerts P., de Wit J., Lamkanfi M., Prinz M, & van Loo G. (2018). A20 critically controls microglia activation and inhibits inflammasome-dependent neuroinflammation. Nature Communications, 9, 2036.
+ Open protocol
+ Expand
3

Protein Markers for Neurodegenerative Disorders

Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary antibodies used: tau (K9J8; Dako, Burlington, ON, Canada; cat. no. A002401-2, 1:1000), phospho-tau (AT8; Thermo Fisher Scientific, Cleveland, OH, USA; cat no. MN1020, 1:1000), APP (clone 22C11; Millipore, Burlington, MA, USA; cat. no. MAB348, 1:1000), vGlut1 (Synaptic Systems, Göttingen, Germany; cat. no. 135 303), β-III-tubulin (BioLegend, San Diego, CA, USA; cat. no. 801202, 1:5000) and Glyceraldehyde 3-phosphate dehydrogenase (Millipore, Burlington, MA, USA; cat. no. MAB374, 1:5000).
Burbulla L.F., Mc Donald J.M., Valdez C., Gao F., Bigio E.H, & Krainc D. (2021). Modeling brain pathology of Niemann-Pick disease type C using patient-derived neurons. Movement disorders : official journal of the Movement Disorder Society, 36(4), 1022-1027.
+ Open protocol
+ Expand
4

Comprehensive Immunostaining Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary antibodies: HT7 (mouse, Thermo Scientific, 1:1,000), AT8 (mouse, Thermo Scientific, 1:1,000), NF200 (rabbit, Sigma–Aldrich, 1:1,000), APP (clone 22C11, mouse, Millipore, 1:1,000), Iba1 (rabbit, Wako, 1:5,000), CD11b (rat, Serotec, 1:1,000), Olig2 (rabbit, Millipore, 1:1,000), NG2 (rabbit, Millipore, 1:1,000) APC (mouse, clone CC1, Chalbiochem, 1:200), SOX2 (goat, Santa Cruz, 1:300), MBP (rat, Serotec, 1:300 in IHC, 1:500 for ICC, and 1:2,000 for WB), βIII‐tubulin (Rabbit, AbCam, 1:500), β‐actin (mouse, Sigma–Aldrich, 1:10,000). Biotin‐conjugated secondary antibodies (Vector, 1:1,000), Alexa‐conjugated secondary antibodies (Molecular Probes, 1:1000), HRP‐conjugated secondary antibodies (1:5000): anti‐mouse and anti‐rabbit (GE), anti‐rat (Vector).
Ossola B., Zhao C., Compston A., Pluchino S., Franklin R.J, & Spillantini M.G. (2015). Neuronal expression of pathological tau accelerates oligodendrocyte progenitor cell differentiation. Glia, 64(3), 457-471.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!