Anti gal 1 antibody

Unless otherwise specified all reagents are from Sigma-Aldrich. Opti-MEM, FluoroBrite™ DMEM, Lipofectamine® transfection reagent, DAPI, CellTrace™ CFSE Cell Proliferation Kit and CellTracker™ Orange Dye are from Invitrogen™ Life Technologies. siRNAs for Gal-1, RNase-free DNase set are from Qiagen. Sh-RNA vectors are purchased from OriGene. RNA Nano Chip kit is from Agilent. PrimeScript RT reagent kit, ProteaseMAX™ Surfactant, Sequencing Grade Modified Trypsin are from Promega. Polyvinylidene difluoride (PVDF) membrane is from Millipore. Transwells are purchased from Euroclone. Anti-Gal-1 antibody is from Cell Signaling, anti-β-actin and anti-Integrin-β1 antibodies are from Santa Cruz Biotechnology, anti-CD81 antibody is from BD Biosciences. The secondary antibodies enzyme horseradish peroxidase (HRP)–conjugated are from Santa Cruz Biotechnology. All materials for SDS-PAGE and Clarity™ Western ECL substrate are from Biorad. Culture-inserts (Dish 35 mm, high) are from ibidi®.

GANT61 was purchased from Sigma Biotechnology (St. Louis, MO, USA). The anti- GAL-1 antibody was purchased from Cell Signaling Technology (13888S and 12936S, Danvers, MA, USA), the anti-GLI1 was purchased from Abcam (ab217326, Cambridge, UK) and Bioss (bs-1206R, Beijing, China), anti-MMP14 (ab78738), anti-MMP2(ab92536), anti-LAMC2 (ab210959 and ab274376) and anti-CD34(ab81289) antibodies were purchased from Abcam (Cambridge, UK), and the anti-GAPDH antibody(sc-47724), horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG(sc-516102) and goat anti-rabbit IgG(sc-2357) were provided by Santa Cruz Biotechnology (Santa Cruz, CA, USA). The PAS staining kit was provided by Leagene Biotechnology Co, Ltd (Beijing, People’s Republic of China). The MTT assay kit and dimethyl sulfoxide (DMSO) were provided by Sigma Biotechnology (St. Louis, MO, USA).