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Horseradish peroxidase hrp type 4

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Horseradish peroxidase (HRP) type IV is an enzyme that catalyzes the oxidation of various substrates in the presence of hydrogen peroxide. It is commonly used as a label or reporter in various biochemical and immunological assays.

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Lab products found in correlation

Dmem without phenol red, by Merck Group (2 mentions) Hanks balanced salt solution (hbss), by Merck Group (2 mentions) Hank s balanced salt solution hbss with mg2 and ca2, by Thermo Fisher Scientific (2 mentions) White 96 well luminometer plate, by Thermo Fisher Scientific (2 mentions) Victor nivo, by PerkinElmer (2 mentions) Victor v3, by PerkinElmer (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Hydrogen peroxide, by Merck Group (1 mentions) Cell culture media, by Thermo Fisher Scientific (1 mentions) Bovine collagen, by Advanced BioMatrix (1 mentions)

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Horseradish peroxidase (696 citations) Horseradish peroxidase (HRP) (213 citations) Type IV (10 citations)

3 protocols using horseradish peroxidase hrp type 4

1

Monitoring Intracellular and Extracellular ROS in Immune Cells

Cited 1 time
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Intra- and extracellular reactive oxygen species (ROS) accumulation was monitored at an MOI of 5:1 using a luminol-dependent chemiluminescence assay as described earlier (Frohner et al., 2009 (link)). Briefly, 4 × 104 BMDMs or neutrophils were suspended in DMEM without phenol red (Sigma-Aldrich) or Hank’s balanced salt solution (HBSS; with Mg2+ and Ca2+; GIBCO) in a well of a white 96-well luminometer plate (Nunc) and mixed with 50 μL HBSS containing 200 μM luminol (intra- and extracellular ROS) or 600 μM isoluminol (extracellular ROS) and 16 U horseradish peroxidase (HRP) type IV (all Sigma-Aldrich). Immediately afterward, 50 μL HBSS containing the indicated C. albicans strains, heat-killed C. albicans, 75 μg Zymosan or 1 μg TDB were added. Chemiluminescence was monitored on a Victor V3 or a Victor Nivo microplate reader (both PerkinElmer). As controls, BMDMs or neutrophils without stimulation and C. albicans alone were included. The detected relative luciferase units (RLU) are expressed as RLU per min per 1000 immune cells or as total RLU after the indicated time.
Jenull S., Mair T., Tscherner M., Penninger P., Zwolanek F., Silao F.G., de San Vicente K.M., Riedelberger M., Bandari N.C., Shivarathri R., Petryshyn A., Chauhan N., Zacchi L.F., LeibundGut -Landmann S., Ljungdahl P.O, & Kuchler K. (2021). The histone chaperone HIR maintains chromatin states to control nitrogen assimilation and fungal virulence. Cell reports, 36(3), 109406.
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2

Monitoring Intracellular and Extracellular ROS in Immune Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Intra- and extracellular reactive oxygen species (ROS) accumulation was monitored at an MOI of 5:1 using a luminol-dependent chemiluminescence assay as described earlier (Frohner et al., 2009 (link)). Briefly, 4 × 104 BMDMs or neutrophils were suspended in DMEM without phenol red (Sigma-Aldrich) or Hank’s balanced salt solution (HBSS; with Mg2+ and Ca2+; GIBCO) in a well of a white 96-well luminometer plate (Nunc) and mixed with 50 μL HBSS containing 200 μM luminol (intra- and extracellular ROS) or 600 μM isoluminol (extracellular ROS) and 16 U horseradish peroxidase (HRP) type IV (all Sigma-Aldrich). Immediately afterward, 50 μL HBSS containing the indicated C. albicans strains, heat-killed C. albicans, 75 μg Zymosan or 1 μg TDB were added. Chemiluminescence was monitored on a Victor V3 or a Victor Nivo microplate reader (both PerkinElmer). As controls, BMDMs or neutrophils without stimulation and C. albicans alone were included. The detected relative luciferase units (RLU) are expressed as RLU per min per 1000 immune cells or as total RLU after the indicated time.
Jenull S., Mair T., Tscherner M., Penninger P., Zwolanek F., Silao F.G., de San Vicente K.M., Riedelberger M., Bandari N.C., Shivarathri R., Petryshyn A., Chauhan N., Zacchi L.F., LeibundGut -Landmann S., Ljungdahl P.O, & Kuchler K. (2021). The histone chaperone HIR maintains chromatin states to control nitrogen assimilation and fungal virulence. Cell reports, 36(3), 109406.
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3

Cell Culture Media Protocol

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Cell culture media, supplements, and materials were purchased from ThermoFisher Scientific unless otherwise noted. Transwells, horseradish peroxidase (HRP) (Type IV), and hydrogen peroxide were purchased from Sigma (St. Louis, MO). Bovine collagen (3mg/mL) was purchased from Advanced Biomatrix (San Diego, CA). Antibodies were purchased from Abcam (Anti-beta III Tubulin, CD68, Alexafluor secondaries) (Cambridge, MA). DAPI was purchased from Sigma.
Lightfoot Vidal S.E., Tamamoto K.A., Nguyen H., Abbott R.D., Cairns D.M, & Kaplan D.L. (2018). 3D Biomaterial Matrix to Support Long Term, Full Thickness, Immunocompetent Human Skin Equivalents with Nervous System Components. Biomaterials, 198, 194-203.
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