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Cd127

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CD127 is a surface marker that is expressed on a subset of lymphocytes, including T cells, B cells, and natural killer cells. It is involved in the regulation of the immune response.

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Lab products found in correlation

Perforin clone pf 344, by Mabtech (2 mentions) Caltag fix perm, by Thermo Fisher Scientific (2 mentions) Il 17a, by Thermo Fisher Scientific (2 mentions) Cd45ra, by Thermo Fisher Scientific (2 mentions) Facs fortessa cytometer, by BD (2 mentions) Cd141, by Miltenyi Biotec (2 mentions) Live dead cell viability assay, by Thermo Fisher Scientific (2 mentions) Ifn γ, by Thermo Fisher Scientific (2 mentions) Ionomycin, by Merck Group (2 mentions) Phorbol myristate acetate (pma), by Merck Group (2 mentions) Granzyme, by BioLegend (1 mentions) Cd11c, by Beckman Coulter (1 mentions) Cd45ra, by Beckman Coulter (1 mentions) Ifn γ, by Beckman Coulter (1 mentions) Il 21, by BioLegend (1 mentions)

5 protocols using cd127

1

Multiparameter Flow Cytometry Analysis

Cited 1 time
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Surface staining was carried out by standard procedures for our laboratory as described [56 (link)]. Except where noted, all reagents were obtained from BD Biosciences (San Diego, CA) and included monoclonal antibodies to the following molecules: CD3 (clone SP34-2, APC-Cy7 conjugate) CD4 (clone SK3, PerCP-Cy5.5 conjugate), CD8α (clone RPA-T8, Alexa700 conjugate) CD28 (clone CD28.2, PE-Texas Red conjugate, Beckman-Coulter, Fullerton, CA), CCR7 (clone 150503, Pacific Blue conjugate, custom) CXCR3 (clone 1C6, PE-Cy5 conjugate), Ki-67 (Clone B56, PE conjugate), CD127 (clone R34.34, PE conjugate, Beckman-Coulter), perforin (clone Pf-344, FITC conjugate, Mabtech, Mariemont, OH). Intracellular staining for perforin expression was performed using Caltag Fix & Perm (Invitrogen, Camarillo, CA) according to the manufacturer’s suggested protocol. Enumeration of SIV-specific cells using PE- or APC-conjugated pentamers to Mamu-A*01 Gag181-189CM9 and Tat28-35SL8 (Proimmune, Oxford, UK) was performed as described previously [57 (link)]. All samples were analyzed using an LSR II (BD Biosciences), and analyses were performed using FlowJo software (Tree Star Inc., Ashland, OR). Isotype-matched controls and/or fluorescence-minus-one (FMO) controls were included in all assays [58 (link)].
Adnan S., Reeves R.K., Gillis J., Wong F.E., Yu Y., Camp J.V., Li Q., Connole M., Li Y., Piatak M J.r., Lifson J.D., Li W., Keele B.F., Kozlowski P.A., Desrosiers R.C., Haase A.T, & Johnson R.P. (2016). Persistent Low-Level Replication of SIVΔnef Drives Maturation of Antibody and CD8 T Cell Responses to Induce Protective Immunity against Vaginal SIV Infection. PLoS Pathogens, 12(12), e1006104.
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2

Multiparameter Profiling of Oral Immune Cells

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Single-cell suspensions from gingival tissues, or buccal mucosa, were untreated or stimulated for 3.5h with or without PMA (50 ng/ml; Sigma) and ionomycin (2.5 μg/ml; Sigma) in the presence of brefeldin A and then stained for cell surface makers and/or intracellular cytokines. Cells were stained with Live/Dead Cell Viability assay (Invitrogen) and different combinations of the following anti-human antibodies: CD34, CD16, CD11c, CD294 (CRTH2), EpCAM (BD Biosciences); CD127 (Beckman Coulter); CD1a, CD3, CD14, CCR7, CD335 (NKp46), CD336 (NKp44), Singlec-8, FcεR1α, (Biolegend); CD1c, HLA-DR, CD45, CD4, CD8, TCRγδ,CD45RO, CD45RA, CD161, CD56, CD19, CD20, CD117, CD15, CD69 , Foxp-3, IL-17A, IFN-γ (eBioscience); and CD141 (Miltenyi Biotec). All samples were analyzed using a FACS Fortessa cytometer (BD Biosciences). Data analysis was performed using FlowJo software (Treestar).
Dutzan N., Konkel J.E., Greenwell-Wild T, & Moutsopoulos N.M. (2016). Characterization of the human immune cell network at the gingival barrier. Mucosal immunology, 9(5), 1163-1172.
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3

Comprehensive Immune Cell Profiling

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Antibodies: CD279 (PD1) (Clone PD1.3 Beckman Coulter), CD45RA (Clone ALB11 Beckman Coulter), CD185 (CXCR5) (Clone J252D4 Biolegend), CD4 (Clone 13B8.2 Beckman Coulter), CD20 (Clone B9E9 Beckman Coulter), CD19 (Clone J4.119 Beckman Coulter), CD38 (Clone HB-7 Biolegend), CD27 (Clone M-T271 Biolegend), IGD (Clone IA6-2 Biolegend), CD11c (Clone BU15 Beckman Coulter), CD14 (Clone RMO52 Beckman Coulter), CD16 (Clone 3G8 Beckman Coulter), CD24 (Clone ALB9 Beckman Coulter), CD45 (Clone J.33 Beckman Coulter), CD3 (Clone UCHT1 Beckman Coulter), CD8 (Clone B9.11 Beckman Coulter), CD56 (Clone N901 Beckman Coulter), CD57 (Clone NC1 Beckman Coulter), CD25 (Clone B1.49.9 Beckman Coulter), CD127 (Clone R34.34 Beckman Coulter), CD159a (NKG2A)(Clone S19004C Biolegend), CD337 (NKp30) (Clone P30-15 Biolegend), IFN-γ (Clone 45.15 Beckman Coulter), IL-21 (Clone 3A3-N2 Biolegend), IL-17 (Clone BL168 Beckman Coulter), granzyme (Clone QA16A02 Biolegend), perforin (Clone B-D48 Biolegend)
Flow Cytometer: Navios, Beckman Coulter
Cytometric Bead Array: RAISECARE; Analysis Software: Kaluza, LEGEND plex.
Jin X., Yan Z.H., Lu L., Lu S., Zhang G, & Lin W. (2021). Peripheral Immune Cells Exhaustion and Functional Impairment in Patients With Chronic Hepatitis B. Frontiers in Medicine, 8, 759292.
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4

Multiparameter flow cytometric analysis

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Surface staining was carried out by standard procedures for our laboratory as described [30 (link)]. Except where noted, all reagents were obtained from BD Biosciences (San Diego, CA) and included monoclonal antibodies to the following molecules: CD3 (clone SP34-2, APC-Cy7 conjugate) CD4 (clone SK3, PerCP-Cy5.5 conjugate), CD8α (clone RPA-T8, Alexa700 conjugate), CD28 (clone CD28.2, PE-Texas Red conjugate, Beckman-Coulter, Fullerton, CA), CCR7 (clone 150503, Pacific Blue conjugate, custom), KI-67 (clone EH12.2H7, PE conjugate, custom), CD127 (clone R34.34, PE conjugate, Beckman-Coulter), perforin (clone Pf-344, FITC conjugate, Mabtech, Mariemont, OH). Intracellular staining for perforin and KI-67 expression was performed using Caltag Fix & Perm (Invitrogen, Camarillo, CA) according to the manufacturer’s suggested protocol. Enumeration of SIV-specific cells using PE- or APC-conjugated tetramers to Mamu-A*01 Gag181–189CM9 and Tat28–35SL8 (kindly provided by Nancy Wilson and David Watkins, Wisconsin National Primate Research Center, Madison Wisconsin) was performed as described previously [31 (link)]. All acquisitions were made on an LSR II (BD Biosciences) and analyses were done using FlowJo software (Tree Star Inc., Ashland, OR). Isotype-matched controls and/or fluorescence-minus-one (FMO) controls were included in all assays [32 (link)].
Adnan S., Colantonio A.D., Yu Y., Gillis J., Wong F.E., Becker E.A., Piatak M J.r., Reeves R.K., Lifson J.D., O’Connor S.L, & Johnson R.P. (2015). CD8 T Cell Response Maturation Defined by Anentropic Specificity and Repertoire Depth Correlates with SIVΔnef-induced Protection. PLoS Pathogens, 11(2), e1004633.
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5

Multiparameter Profiling of Oral Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Single-cell suspensions from gingival tissues, or buccal mucosa, were untreated or stimulated for 3.5h with or without PMA (50 ng/ml; Sigma) and ionomycin (2.5 μg/ml; Sigma) in the presence of brefeldin A and then stained for cell surface makers and/or intracellular cytokines. Cells were stained with Live/Dead Cell Viability assay (Invitrogen) and different combinations of the following anti-human antibodies: CD34, CD16, CD11c, CD294 (CRTH2), EpCAM (BD Biosciences); CD127 (Beckman Coulter); CD1a, CD3, CD14, CCR7, CD335 (NKp46), CD336 (NKp44), Singlec-8, FcεR1α, (Biolegend); CD1c, HLA-DR, CD45, CD4, CD8, TCRγδ,CD45RO, CD45RA, CD161, CD56, CD19, CD20, CD117, CD15, CD69 , Foxp-3, IL-17A, IFN-γ (eBioscience); and CD141 (Miltenyi Biotec). All samples were analyzed using a FACS Fortessa cytometer (BD Biosciences). Data analysis was performed using FlowJo software (Treestar).
Dutzan N., Konkel J.E., Greenwell-Wild T, & Moutsopoulos N.M. (2016). Characterization of the human immune cell network at the gingival barrier. Mucosal immunology, 9(5), 1163-1172.
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