Carl zeissaxioimager microscope

Manufactured by Zeiss

Sourced in Germany

The Carl Zeiss AxioImager microscope is a high-performance optical microscope designed for advanced research applications. It features a modular design and supports a range of contrast techniques, including brightfield, darkfield, and differential interference contrast (DIC). The AxioImager provides precise control over illumination and offers a range of objectives to accommodate diverse sample types.

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Lab products found in correlation

Brdu red dna fragmentation assay kit, by Abcam (1 mentions) Image m1, by Zeiss (1 mentions) Collagen 1, by Abcam (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)

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Carl Zeiss microscope

2 protocols using carl zeissaxioimager microscope

Immunofluorescence Microscopy Imaging of Apoptosis

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Paraffin-embedded tumor tissues were sectioned to a thickness of 5 μm,
deparaffinized with xylene, rehydrated through xylene and graded alcohol and
blocked with 5% bovine serum albumin. Immunofluorescence (IF) staining was
conducted with the indicated antibodies and fluorochrome-conjugated secondary
antibodies (Alexa-488 and 564). Apoptotic cells were identified by terminal
deoxynucleotidyl transfer-mediated dUTP nick-end labeling (TUNEL) staining using
an in situ BrdU-Red DNA fragmentation assay kit (Abcam,
Cambridge, UK). Nuclei were counterstained with DAPI. The slides were examined
in a blinded manner and randomly chosen fields were photographed at 400x
magnification. The immune-positive cells were quantified with a Carl Zeiss
AxioImager microscope and Image M1 Software (Carl Zeiss, Jena, Germany).

Lee H.S., Jung J.I., Kim K.H., Park S.J, & Kim E.J. (2020). Toxicodendron vernicifluum Stokes extract inhibits solid tumor growth and lung metastasis of 4T1 murine mammary carcinoma cells in BALB/c mice. PLoS ONE, 15(11), e0241805.

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Melanin and Collagen 1 Analysis in Skin Tissue

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Dorsal skin tissues were fixed in 4% paraformaldehyde immediately after removal. The fixed skin tissues were embedded in paraffin, sectioned into 5-µm-thick slices, deparaffinized with xylene, and rehydrated using xylene and graded alcohol. To observe the changes in the melanin content in the skin tissue, Fontana-Masson’s silver (Abcam, Cambridge, UK) staining was performed following the manufacturer’s instructions. In addition, to investigate collagen 1 expression, IF staining was conducted with a primary antibody (collagen 1; Abcam) and fluorochrome-conjugated secondary antibody (anti-rabbit IgG-Alexa-488; Thermo Fisher Scientific). Nuclei were counterstained with 4',6-diamidino-2-phenylindole (Sigma-Aldrich, St. Louis, MO, USA). The stained slides were examined in a blind manner, and randomly chosen fields were photographed under a Carl Zeiss Axio Imager microscope (Carl Zeiss, Jena, Germany).

Kim Y.H., Lim C.Y., Jung J.I., Kim T.Y, & Kim E.J. (2023). Protective effects of red orange (Citrus sinensis [L.] Osbeck [Rutaceae]) extract against UVA-B radiation-induced photoaging in Skh:HR-2 mice. Nutrition Research and Practice, 17(4), 641-659.

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