7 0 vicryl

After laryngofissure was performed under general anesthesia with endotracheal intubation, the unilateral membranous portion of the vocal fold was resected according to the cordectomy type II method proposed by the European Laryngological Society in 2000 [17 (link)] (Fig 2A and 2B). Cordectomy was performed by cutting between the vocal mucosa and vocalis muscle, i.e., resection of the full depth of the lamina propria. The vocalis muscle was preserved to the extent possible. The resection extended from the vocal process to the anterior commissure. The superior to inferior dimensions of the resection were about one-third of the vocal fold thickness. In the five dogs for which mucosae were harvested, the resected area was immediately covered with tissue-engineered vocal fold mucosa using fibrin glue and fixed with absorbable surgical sutures (7–0 Vicryl, Ethicon) in four locations (anterior, posterior, superior, and inferior) under an operating microscope. During this time, the fabricated organotypic cultured tissue was transplanted along the same direction as the vocal fold. The antibiotics cefazolin sodium hydrate (20 mg/kg/day; for prevention of infection) and acetaminophen (10 mg/kg; analgesic) were administered after surgery.

This study was approved by the Animal Ethics Committee of Inha University Hospital (INHA 18 0503-560) and animal care and experiments were performed in accordance with established institutional animal ethics committee regulations. Twenty-two female New Zealand white rabbits about 2 kg at 12 weeks of age were randomly assigned to a non-loaded NGC group (n = 10) or a PRP-loaded NGC tube group (n = 12). The right RLNs of rabbits were preserved as normal controls, but the left RLNs were resected and interposed with an NGC (Fig. 1e). Preoperatively, animals were anesthetized with 5 mg/kg subcutaneous xylazine, and immediately prior to surgery, an intramuscular injection of 15 mg/kg zolazepam was administered. In each case, a cervical vertical incision was used to expose the left RLN, which was then dissected circumferentially (Fig. 1f, g). A 10-mm length was resected. An NGC was interposed between the proximal and distal nerve endings and attached using junction sutures (7-0 Vicryl; Ethicon, Somerville, NJ, USA). A total of 10 rabbits underwent non-loaded NGC tube interposition and 12 rabbits underwent PRP-loaded NGC tube interposition. After implantation, the strap muscles were closed with a 4-0 Vicryl suture, and the skin was closed with 3-0 Ethilon (Ethicon, Somerville, NJ).