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Minelute dna purification kit

Manufactured by Qiagen
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The MinElute DNA Purification Kit is a laboratory equipment product designed for the efficient purification of DNA. It utilizes a silica-membrane-based technology to facilitate the extraction and concentration of DNA samples from various sources. The core function of this kit is to provide a reliable and convenient method for DNA purification, enabling users to obtain high-quality DNA samples for downstream applications.

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Lab products found in correlation

Hiseq 2500, by Illumina (2 mentions) Nextseq 500, by Illumina (2 mentions) Nextera dna library preparation kit, by Illumina (2 mentions) Tagment dna enzyme 1, by Illumina (2 mentions) Steponeplus qpcr machine, by Thermo Fisher Scientific (1 mentions) Sybr green, by Thermo Fisher Scientific (1 mentions) H3k4me3, by Diagenode (1 mentions) Quant it picogreen dsdna assay kit, by Thermo Fisher Scientific (1 mentions) Gs junior titanium empcr kit lib l, by Roche (1 mentions)

Close spelling variants of this product

MinElute kit (274 citations) MinElute purification kit (34 citations) DNA kits (7 citations)

6 protocols using minelute dna purification kit

1

ATAC-seq Protocol for Chromatin Accessibility

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ATAC-seq was performed as previously described32 (link). 100,000 cells were washed once with 100 μl PBS and resuspended in 50 μl lysis buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.2% IGEPAL CA-630). Cells were centrifuged for 10 min at 500g (4°C), supernatant was removed and nuclei were resuspended in 50 μl transposition reaction mix (25 μl TD buffer, 2.5 μl Tn5 transposase and 22.5 μl nuclease-free water) and incubated at 37°C for 45 min. DNA was isolated using the MinElute DNA Purification Kit (Qiagen). Library amplification was performed by two sequential PCR reactions (8 and 5 cycles, respectively). Library quality was checked on a Bioanalyzer, followed by paired-end sequencing (2x75bp) on an Illumina HiSeq2500.
Stadhouders R., Vidal E., Serra F., Di Stefano B., Le Dily F., Quilez J., Gomez A., Collombet S., Berenguer C., Cuartero Y., Hecht J., Filion G.J., Beato M., Marti-Renom M.A, & Graf T. (2018). Transcription factors orchestrate dynamic interplay between genome topology and gene regulation during cell reprogramming. Nature genetics, 50(2), 238-249.
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2

ATAC-seq Protocol for Chromatin Accessibility

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ATAC-seq was performed as previously described32 (link). 100,000 cells were washed once with 100 μl PBS and resuspended in 50 μl lysis buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.2% IGEPAL CA-630). Cells were centrifuged for 10 min at 500g (4°C), supernatant was removed and nuclei were resuspended in 50 μl transposition reaction mix (25 μl TD buffer, 2.5 μl Tn5 transposase and 22.5 μl nuclease-free water) and incubated at 37°C for 45 min. DNA was isolated using the MinElute DNA Purification Kit (Qiagen). Library amplification was performed by two sequential PCR reactions (8 and 5 cycles, respectively). Library quality was checked on a Bioanalyzer, followed by paired-end sequencing (2x75bp) on an Illumina HiSeq2500.
Stadhouders R., Vidal E., Serra F., Di Stefano B., Le Dily F., Quilez J., Gomez A., Collombet S., Berenguer C., Cuartero Y., Hecht J., Filion G.J., Beato M., Marti-Renom M.A, & Graf T. (2018). Transcription factors orchestrate dynamic interplay between genome topology and gene regulation during cell reprogramming. Nature genetics, 50(2), 238-249.
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3

ATAC-Seq on FACS-Purified Cell Samples

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ATAC-Seq was performed as previously described22 (link) on 5,000–40,000 FACS-purified cells from 2–9 mice. In brief, cells were lysed in lysis buffer for 1 minute and transposed with Tagment DNA Enzyme 1 (Illumina) for 30 minutes. DNA was cleaned up using a MinElute DNA purification Kit (Qiagen), followed by barcoding and library preparation by the Nextera DNA Library preparation kit (Illumina) according to manufacturer’s guidelines and sequenced on an Illumina NextSeq500.
London M., Bilate A.M., Castro T.B., Sujino T, & Mucida D. (2021). Stepwise chromatin and transcriptional acquisition of an intraepithelial lymphocyte program. Nature immunology, 22(4), 449-459.
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4

ChIP-qPCR Analysis of H3K4me3

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Trained monocytes on day 6 were cross-linked in methanol-free 1% formaldehyde, followed by sonication and immunoprecipitation using antibodies against H3K4me3 (Diagenode, Seraing, Belgium). Immunoprecipitated chromatin was processed further for qRT-PCR analysis using the MinElute DNA Purification Kit (Qiagen). Primers used in the reaction are listed in Table S1. Samples were analyzed with a comparative Ct method on the StepOnePLUS qPCR machine (Applied Biosystems) using SYBR green (Invitrogen) in accordance with the manufacturer’s instructions.
Keating S.T., Groh L., Thiem K., Bekkering S., Li Y., Matzaraki V., van der Heijden C.D., van Puffelen J.H., Lachmandas E., Jansen T., Oosting M., de Bree L.C., Koeken V.A., Moorlag S.J., Mourits V.P., van Diepen J., Strienstra R., Novakovic B., Stunnenberg H.G., van Crevel R., Joosten L.A., Netea M.G, & Riksen N.P. (2020). Rewiring of glucose metabolism defines trained immunity induced by oxidized low-density lipoprotein. Journal of Molecular Medicine (Berlin, Germany), 98(6), 819-831.
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5

ATAC-Seq for Chromatin Accessibility Analysis

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ATAC-Seq was performed as previously described 22, 52 on 5,000-40,000 FACS-purified cells from 2-9 mice. In brief, cells were lysed in lysis buffer for 1 minute and transposed with Tagment DNA Enzyme 1 (Illumina) for 30 minutes. DNA was cleaned up using a MinElute DNA purification Kit (Qiagen), followed by barcoding and library preparation by the Nextera DNA Library preparation kit (Illumina) according to manufacturer's guidelines and sequenced on an Illumina NextSeq500.
was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
The copyright holder for this preprint (which this version posted June 5, 2020. ; https://doi.org/10.1101/2020.06.04.134650 doi: bioRxiv preprint 20
London M., Bilate A.M., Castro T.B.R., & Mucida D. (2020). Stepwise chromatin and transcriptional acquisition of an intraepithelial lymphocyte program.
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6

Targeted Amplicon Sequencing of SCN1A

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SCN1A amplicons of each patient were pooled at equal molecule amounts and purified by MinElute DNA purification kit (Qiagen, ABD). Ten nucleotides long MID sequences specifying each patient were ligated to amplicons in each pool using GS Rapid Library Preperation Kit Lib-L (Roche, Germany) as described in the GS Junior Rapid Library Preparation Manual. Concentration of pooled amplicons was measured both by Quant-iT-PicoGreen dsDNA assay Kit (Invitrogen, ABD) and also by qPCR using KAPA NGS quantification kit (KAPA systems, ABD) on LightCycler 480II. Dilutions were made to have single fragment per bead and the sequencing library was prepared for emulsion PCR (emPCR) using GS Junior Titanium emPCR Kit Lib-L (Roche, Germany) as described in GS Junior emPCR Lib-L manual. DNA attached beads were picked up magnetically and pyrosequenced using GS Junior sequencing kit by following the instructions in the GS Junior sequencing method manual. Amplicon sequences were analyzed by Amplicon Variant Analyzer (AVA) program (Roche, Gernmany) using the SCN1A reference sequence, PCR primer and MID sequence information. In the result of AVA analysis, a variant list was obtained for each patient. The variants were filtered for known SNPs and unique variants were validated by Sanger sequencing.
Usluer S., Salar S., Arslan M., Yiş U., Kara B., Tektürk P., Baykan B., Meral C., Türkdoğan D., Bebek N., Yalçın Çapan Ö., Gündoğdu Eken A, & Çağlayan S.H. (2016). SCN1A gene sequencing in 46 Turkish epilepsy patients disclosed 12 novel mutations. Seizure, 39.
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