Ds fi3 5.9 mp colour camera

Manufactured by Nikon

The DS-Fi3 is a 5.9 MP colour camera designed for laboratory applications. It features a CMOS image sensor and can capture images at resolutions up to 2880 x 2048 pixels.

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Lab products found in correlation

Smz1270, by Nikon (1 mentions) Smz1270 stereo microscope, by Nikon (1 mentions)

2 protocols using ds fi3 5.9 mp colour camera

Heat Stress Response in Fasted Worms

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Animals were synchronised by egg lay for 1 hour at 20°C. Once animals reached the L4 stage, they were transferred onto NGM plates with or without food for 24 hours. After this period, worms were moved back to plates containing food for 24 hours, at which point, fed and fasted animals were heat-shocked in a water bath for 4 hours at 35°C, or kept at 20°C for an equivalent period of time. Immediately following heat shock, worms were mounted onto slides for image acquisition. Worms were mounted on 2% agarose pads in 3 mM levamisole and imaged immediately. For image acquisition, a Nikon SMZ1270 fluorescence dissecting stereomicroscope with a DS-Fi3 5.9 MP colour camera was used. Images were processed using ImageJ.⁶⁶ (link)

Tataridas-Pallas N., Aman Y., Williams R., Chapman H., Cheng K.J., Gomez-Paredes C., Bates G.P, & Labbadia J. (2024). Mitochondrial clearance and increased HSF-1 activity are coupled to promote longevity in fasted Caenorhabditis elegans. iScience, 27(6), 109834.

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Lipid Quantification in C. elegans

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Staining for lipid levels was essentially performed as previously described. 60 Breifly, worms were washed 3 times in M9 buffer, fixed immediately in 60% isopropanol at room temperature, and then stained with freshly filtered ORO (60% ORO working solution) overnight at 25 C. Oil Red O solution was then washed-out using M9-0.01% Triton-X and worms were mounted on 3% agarose pads.
Images were acquired on a Nikon SMZ1270 stereo microscope with a DS-Fi3 5.9 MP colour camera. Images were processed using ImageJ.

Aman Y., Erinjeri A.P., Tataridas-Pallas N., Williams R., Wellman R., Chapman H, & Labbadia J. (2022). Loss of MTCH-1 suppresses age-related proteostasis collapse through the inhibition of programmed cell death factors. Cell reports, 41(8).

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