Chondrocytes were cultured in various formats: 96-well plates for cell proliferation assay, 6-well plates for cell viability and cytotoxicity, cell apoptosis detection, measurement of ROS, detection of nitric oxide (NO), mitochondrial membrane potential (MMP) measurement, cytochrome C release and protein experiments, and 6-cm dishes for mRNA extraction. Upon reaching confluence, OA chondrocytes were pretreated with 10 μM GW1929 (Med Chem Express, Shanghai, China) or 10 μM Z-DEVD-FMK (AdooQ Bioscience, Irvine, CA, USA) for 1 h.
Z devd fmk
Manufactured by Adooq
Sourced in China, United States
Z-DEVD-FMK is a laboratory reagent used for research purposes. It functions as a caspase-3 inhibitor, a key enzyme involved in apoptosis, or programmed cell death. The product is intended for in vitro use only and its specific applications should be determined by the researcher.
Automatically generated - may contain errors
Lab products found in correlation
Gw1929, by MedChemExpress (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Proliferating cell nuclear antigen (pcna), by Abcam (1 mentions)
Gapdh, by Abcam (1 mentions)
Necrostatin 1, by MedChemExpress (1 mentions)
Caspase 8, by Cell Signaling Technology (1 mentions)
Caspase 3, by Cell Signaling Technology (1 mentions)
Caspase 9, by Cell Signaling Technology (1 mentions)
Oxaliplatin, by MedChemExpress (1 mentions)
Cytochrome c, by Cell Signaling Technology (1 mentions)
Vdac1, by Cell Signaling Technology (1 mentions)
5 fluorouracil, by MedChemExpress (1 mentions)
Goat igg hrp secondary antibodies against rabbit and mouse, by Zhongshan Golden Bridge Biotechnology (1 mentions)
Fitc or cy3 conjugated secondary antibodies, by Merck Group (1 mentions)
Ripk1, by Cell Signaling Technology (1 mentions)
Z vad fmk, by MedChemExpress (1 mentions)
N acetyl l cysteine, by Beyotime (1 mentions)
L glutathione, by Beyotime (1 mentions)
2 protocols using z devd fmk
1
OA Chondrocyte Pretreatment Assays
2
Apoptosis and Necroptosis Pathways Evaluation
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SHK was purchased from Sigma-Aldrich (St. Louis, MO, USA), dissolved in DMSO (Sigma-Aldrich, St. Louis, MO, USA). ZVAD-FMK, Necrostatin-1, 5-Fluorouracil and Oxaliplatin were from MedChemExpress (NJ, USA). ZDEVD-FMK and ZLEHD-FMK were from Adooq bioscience (CA, USA). N-acetyl-L-cysteine and L-glutathione were from Beyotime Institute (Shanghai, China). Antibodies were as follows: PARP, Caspase 3, Caspase 8, Caspase 9, RIPK1, Cytochrome C and VDAC1 were from Cell Signaling Technology (MA, USA); AIF, Endonuclease G, GAPDH and PCNA were from Abcam (Cambridge, UK); the goat IgG-HRP secondary antibodies against rabbit and mouse were from Zhongshan Golden Bridge Biotechnology (Peking, China) and FITC or Cy3 conjugated secondary antibodies were from Sigma (MO, USA).
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