Ab241566

Manufactured by Abcam

Sourced in United States

Ab241566 is a lab equipment product. It is a device used for laboratory research and analysis. The core function of this product is to perform specific tasks in a controlled and precise manner within a laboratory setting.

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Lab products found in correlation

2 protocols using ab241566

Characterization of MCM Protein Expression

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For WB, the detailed procedures were performed as described previously [8] using the following primary antibodies: anti-MCM2 (Abcam, ab4461), anti-MCM3 (Abcam, ab128923), anti-MCM4 (Abcam, ab4459), anti-MCM5 (Abcam, ab75975), anti-MCM6 (Abcam, ab201683), anti-MCM7 (Abcam, ab2360), anti-CDK9 (Abcam, ab76320), anti-CyclinD1 (Abcam, ab16663), anti-CyclinE1 (Abcam, ab33911), anti-p53 (Abcam, ab241566), anti-p21 (Abcam, ab109502), anti-p27 (Abcam, ab32034), and anti-GAPDH (Abcam, ab181602).
For IHC, the standard method was described previously.14 (link) Slides were incubated with primary antibodies (Abs) against MCM2 (Immuway, YM6642), MCM3 (Abcam, ab128923), MCM4 (Immuway, YT2681), MCM5 (Abcam, ab75975), MCM6 (Abcam, ab190948), and MCM7 (Abcam, ab2360).

Niu Y., Shan L., Gao H., Zhang C., Qian Z., Wang Z., Xu X., Zhang X., Wang J., Ma L., Chen L, & Yu Y. (2020). Huaier Suppresses the Hepatocellular Carcinoma Cell Cycle by Regulating Minichromosome Maintenance Proteins. OncoTargets and therapy, 13, 12015-12025.

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Western Blot Analysis of Key Signaling Proteins

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After transfection for 72 h, the total protein of cells lysed with RIPA lysis buffer (Beyotime, China) was determined by the BCA detection kit (Beyotime). Next, protein (30 µg) was isolated via 10% SDS-PAGE and transferred to PVDF membrane (Millipore, USA), and then blocked with 5% skimmed milk at 25 °C for 2 h. After removal, membranes were incubated with primary antibody: anti-p53 (ab241566; Abcam), p-STAT3 (ab76351; Abcam), STAT3 (ab68153; Abcam), p-JAK2 (ab32101; Abcam), JAK2 (ab39636; Abcam), GAPDH (ab8245; Abcam) at 4 °C overnight. After washing, the membrane was incubated with HRP-labeled secondary antibody 25 °C for 1 h, and then the signal strength of the proteins was analyzed using the ECL detection system (Millipore, USA) and Quantity One software (Bio-Rad, USA).

Bao D., Zhuang C., Jiao Y, & Yang L. (2022). The possible involvement of circRNA DMNT1/p53/JAK/STAT in gestational diabetes mellitus and preeclampsia. Cell Death Discovery, 8, 121.

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