Log In Sign up
The largest database of trusted experimental protocols

Annexin 5 assay

Manufactured by BD
Visit Supplier
Sourced in United Kingdom

The Annexin V assay is a laboratory technique used to detect and quantify the presence of Annexin V, a protein that binds to phosphatidylserine. This assay is commonly used to study apoptosis, or programmed cell death, in various cell types.

Automatically generated - may contain errors

Lab products found in correlation

Facscan, by BD (4 mentions) Facscalibur, by BD (1 mentions) Crenolanib, by Selleck Chemicals (1 mentions) Midostaurin, by Selleck Chemicals (1 mentions) Facscan flow cytometer, by BD (1 mentions) Entrectinib, by Selleck Chemicals (1 mentions) Dasatinib, by Selleck Chemicals (1 mentions) Nerve growth factor (ngf), by Thermo Fisher Scientific (1 mentions) Hmc 1, by Merck Group (1 mentions) Dcfda assay, by Abcam (1 mentions) Staphylococcus aureus bioparticles, by Thermo Fisher Scientific (1 mentions) Propidium iodide, by Merck Group (1 mentions) Facsaria 2 cell sorter, by BD (1 mentions) Macsquant analyzer 10 cytometer, by Miltenyi Biotec (1 mentions) Ghost red, by Cytek Biosciences (1 mentions) Cytofix cytoperm, by BD (1 mentions) Perm wash, by BD (1 mentions) Aria 2 cytometer, by BD (1 mentions) Flica poly caspase assay kit, by ImmunoChemistry Technologies (1 mentions) Celltrace violet ctv, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Annexin V-FITC Apoptosis Detection assay Annexin V-FITC/PI Apoptosis Assay Kit Annexin V-FITC apoptosis assay kit Annexin-V/fluorescein isothiocyanate (FITC) assay Annexin V-PE assay kit APC-Annexin-V assay Annexin V-FITC Apoptosis Detection assay kit I Annexin V-FITC binding assay kit II Annexin V-fluorescein isothiocyanate (FITC)-binding assay Annexin V-fluorescein isothiocyanate (FITC) apoptosis assay kit

14 protocols using annexin 5 assay

1

Annexin V Assay for Apoptosis in RA

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The detection of apoptosis by annexin V assay (BD Pharmingen) was performed on PBMCs isolated from RA patients and HC cultured in serum-free medium for 48 hours as previously described [19 (link)]. Briefly, PBMCs were washed twice with ice cold phosphate buffered saline (PBS) and resuspended in 1X annexin binding buffer containing annexin V as per manufacturer’s instructions (BD Pharmingen). Cells were gently vortexed and incubated at room temperature for 15 minutes in dark. Cell suspension volume was made up to 500μl with 1X annexin V binding buffer and analyzed in flow cytometer (BD FACS Calibur) for annexin V staining.
Wendling D., Abbas W., Godfrin-Valnet M., Kumar A., Guillot X., Khan K.A., Vidon C., Coquard L., Toussirot E., Prati C, & Herbein G. (2015). Dysregulated Serum IL-23 and SIRT1 Activity in Peripheral Blood Mononuclear Cells of Patients with Rheumatoid Arthritis. PLoS ONE, 10(3), e0119981.
+ Open protocol
+ Expand
2

Apoptosis Assay in X-Ray-Exposed CD34+ Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Apoptosis detection was done by Annexin-V assay (#556547, BD Biosciences) in human CD34+ cells were exposed to 4 Gy X-ray or Orientin (0.1–5 µM) + 4 Gy.
Yadav M., Song F., Huang J., Chakravarti A, & Jacob N.K. (2018). Ocimum flavone Orientin as a countermeasure for thrombocytopenia. Scientific Reports, 8, 5075.
+ Open protocol
+ Expand
3

Apoptosis Analysis in Leukemic Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Leukemic cells were cultured in the presence of inhibitors for 48 hours before apoptosis analysis. Cell viability was analyzed using the Annexin-V assay (BD Pharmingen, Heidelberg, Germany) on an FCASCanto. We generally measured and analyzed at least 10,000 cells per tube. Annexin V+/PI- and Annexin V+/PI+ cells are considered cells in the early stage of apoptosis and the late-stage of apoptosis, respectively. The inhibitors crenolanib and midostaurin were purchased from Selleckchem (Houston, TX).
Pan Z., Yang M., Huang K., Büsche G., Glage S., Ganser A, & Li Z. (2017). Flow cytometric characterization of acute leukemia reveals a distinctive “blast gate” of murine T-lymphoblastic leukemia/lymphoma. Oncotarget, 9(2), 2320-2328.
+ Open protocol
+ Expand
4

Quantifying Apoptosis via AnnexinV

Check if the same lab product or an alternative is used in the 5 most similar protocols
An AnnexinV assay (BD) was performed according to the manufacturer’s protocols to assess apoptosis. Briefly, cells were harvested after transfection with either the wild-type or mutant SETD1B plasmids, washed twice with PBS, incubated with FITC-conjugated AnnexinV for 15 min, and measured by flow cytometry (FCM) using the FACScan flow cytometer (BD).
Yang P., Huang X., Lai C., Li L., Li T., Huang P., Ouyang S., Yan J., Cheng S., Lei G., Wang Z., Yu L., Hong Z., Li R., Dong H., Wang C., Yu Y., Wang X., Li X., Wang L., Lv F., Yin Y., Yang H., Song J., Gao Q., Wang X, & Zhang S. (2019). SET domain containing 1B gene is mutated in primary hepatic neuroendocrine tumors. International journal of cancer, 145(11), 2986-2995.
+ Open protocol
+ Expand
5

Clonal Growth Analysis of Mast Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
To analyze clonal growth, HMC-1 cells and HMC-1.2 (purchased from Millipore) were plated in H4230 media (StemCell Technologies, Vancouver, Canada) in the presence of kinase inhibitors. The cells were plated as duplicates or quadruplicates. HMC-1 cells with ectopic expression of TRKB were generated by retroviral transduction using a vector expressing human TRKB [12 (link)]. We performed PCR for detection of mycoplasma in cell cultures [57 (link)] and did not detect contamination of mycoplasma in tested cell lines. Primary mast cells were cultured in the presence of inhibitors for 48 hours before apoptosis analysis. Cell viability was analyzed using the Annexin-V assay (BD Pharmingen, Heidelberg, Germany). Inhibitors dasatinib and entrectinib were purchased from Selleckchem (Houston, TX). NGF and BDNF were purchased from PeproTech. We chose dasatinib for KIT inhibition in this study, since dasatinib treatment showed benefit in some patients with SM [5 (link), 58 (link)], and dasatinib is currently being tested in our Phase III clinical trial for CBF AML including KIT D816V mutated patients (ClinicalTrials.gov number, NCT02013648).
Yang M., Pan Z., Huang K., Büsche G., Feuerhake F., Chaturvedi A., Nie D., Heuser M., Thol F., von Neuhoff N., Ganser A, & Li Z. (2017). Activation of TRKA receptor elicits mastocytosis in mice and is involved in the development of resistance to KIT-targeted therapy. Oncotarget, 8(43), 73871-73883.
+ Open protocol
+ Expand
6

Combinatorial ASO and Drug Screening

Check if the same lab product or an alternative is used in the 5 most similar protocols
KMS11, JJN3, MM1R, H929, and RPMI-8226 cells were treated with NT or ILF2 ASOs for 1 or 3 weeks prior to receiving either ASOs alone or ASOs in combination with melphalan, bortezomib, IACS-010759, brequinar, or NSC at the concentrations and times specified in the figure legends. The frequencies of apoptotic cells were determined by flow cytometry using the annexin-V assay (BD Bioscience, #88-8007-74).
Thongon N., Ma F., Baran N., Lockyer P., Liu J., Jackson C., Rose A., Furudate K., Wildeman B., Marchesini M., Marchica V., Storti P., Todaro G., Ganan-Gomez I., Adema V., Rodriguez-Sevilla J.J., Qing Y., Ha M.J., Fonseca R., Stein C., Class C., Tan L., Attanasio S., Garcia-Manero G., Giuliani N., Berrios Nolasco D., Santoni A., Cerchione C., Bueso-Ramos C., Konopleva M., Lorenzi P., Takahashi K., Manasanch E., Sammarelli G., Kanagal-Shamanna R., Viale A., Chesi M, & Colla S. (2024). Targeting DNA2 overcomes metabolic reprogramming in multiple myeloma. Nature Communications, 15, 1203.
+ Open protocol
+ Expand
7

Annexin V Apoptosis Assay by FCM

Check if the same lab product or an alternative is used in the 5 most similar protocols
Annexin V assay (BD Pharmingen) was performed to assess apoptosis according to the manufacturer's instructions. Briefly, cells were harvested after exposure to KPT-330, washed twice with PBS, incubated with FITC/PE-conjugated Annexin V and propidium iodide/7-AAD for 15 min, and measured by flow cytometry (FCM) using FACScan (Becton Dickinson).
Lin D.C., Hao J.J., Nagata Y., Xu L., Shang L., Meng X., Sato Y., Okuno Y., Varela A.M., Ding L.W., Garg M., Liu L.Z., Yang H., Yin D., Shi Z.Z., Jiang Y.Y., Gu W.Y., Gong T., Zhang Y., Xu X., Kalid O., Shacham S., Ogawa S., Wang M.R, & Koeffler H.P. (2014). Genomic and molecular characterization of esophageal squamous cell carcinoma. Nature genetics, 46(5), 467-473.
+ Open protocol
+ Expand
8

Annexin V Apoptosis Assay by FCM

Check if the same lab product or an alternative is used in the 5 most similar protocols
Annexin V assay (BD Pharmingen) was performed to assess apoptosis according to the manufacturer's instructions. Briefly, cells were harvested after exposure to KPT-330, washed twice with PBS, incubated with FITC/PE-conjugated Annexin V and propidium iodide/7-AAD for 15 min, and measured by flow cytometry (FCM) using FACScan (Becton Dickinson).
Lin D.C., Hao J.J., Nagata Y., Xu L., Shang L., Meng X., Sato Y., Okuno Y., Varela A.M., Ding L.W., Garg M., Liu L.Z., Yang H., Yin D., Shi Z.Z., Jiang Y.Y., Gu W.Y., Gong T., Zhang Y., Xu X., Kalid O., Shacham S., Ogawa S., Wang M.R, & Koeffler H.P. (2014). Genomic and molecular characterization of esophageal squamous cell carcinoma. Nature genetics, 46(5), 467-473.
+ Open protocol
+ Expand
9

Evaluation of Immune Cell Function

Check if the same lab product or an alternative is used in the 5 most similar protocols
Viability was assessed using CellTiter 96 AQueous One Solution (Roche, UK). Apoptosis was assessed by flow cytometry using an Annexin V assay (BD Biosciences, UK) in combination with the vital dye propidium iodide (PI) (Sigma-Aldrich, UK). CXCL-8, interleukin (IL)-6, monocyte chemoattractant protein (MCP)-1, tumour necrosis factor (TNF)-α and matrix metalloproteinase (MMP)-9 levels in cell-free supernatants were quantified using commercially available ELISA kits (Biotechne, UK). Reactive oxygen species (ROS) were measured using DCFDA assay (Abcam ab113851) according to the manufacturer’s instructions. Phagocytosis assay was carried out using pHrodo Red Escherichia coli or Staphylococcus aureus BioParticles (Invitrogen, UK) according to the manufacturer’s instructions.
Scott A., Lugg S.T., Aldridge K., Lewis K.E., Bowden A., Mahida R.Y., Grudzinska F.S., Dosanjh D., Parekh D., Foronjy R., Sapey E., Naidu B, & Thickett D.R. (2018). Pro-inflammatory effects of e-cigarette vapour condensate on human alveolar macrophages. Thorax, 73(12), 1161-1169.
+ Open protocol
+ Expand
10

Annexin V Assay for Apoptosis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Apoptosis was assessed using the Annexin V assay (BD Biosciences) on the BD FACScan according to manufacturer's instructions. Assay was conducted 96 h post silencing and cells were stained with propidium iodide (PI) and anti-annexin V. During analysis cells were divided into quadrants representing healthy cells, necrotic cells and early and late apoptotic cells.
McClurg U.L., Harle V.J., Nabbi A., Batalha-Pereira A., Walker S., Coffey K., Gaughan L., McCracken S.R, & Robson C.N. (2015). Ubiquitin-specific protease 12 interacting partners Uaf-1 and WDR20 are potential therapeutic targets in prostate cancer. Oncotarget, 6(35), 37724-37736.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!