Isopropylthiogalactopyranoside iptg

Manufactured by Merck Group

Sourced in Poland, United States

Isopropylthiogalactopyranoside (IPTG) is a synthetic chemical compound commonly used in molecular biology laboratories. It serves as an inducer molecule, playing a key role in the regulation of gene expression in bacterial systems.

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Lab products found in correlation

Histrap hp column, by Cytiva (1 mentions) Kanamycin, by Merck Group (1 mentions) Superdex 200, by Cytiva (1 mentions) Q sepharose ff, by Cytiva (1 mentions) Sp sepharose ff, by Cytiva (1 mentions) 12 o tetradecanoylphorbol 13 acetate pma, by Merck Group (1 mentions) Pravastatin sodium salt, by Cayman Chemical (1 mentions) Adenosine 5 monophosphate disodium salt, by Merck Group (1 mentions) Adenosine 5 triphosphate disodium salt hydrate, by Merck Group (1 mentions) Simvastatin, by Cayman Chemical (1 mentions) Adenosine 5 diphosphate sodium salt, by Merck Group (1 mentions) Tetrabutylammonium hydrogensulfate tba, by Merck Group (1 mentions) Methanol, by Merck Group (1 mentions) Potassium phosphate monobasic kh2po4, by Avantor (1 mentions) Ethanol, by Avantor (1 mentions) Gold nanoparticle solution, by Merck Group (1 mentions) Polyethylene oxide peo powder, by Merck Group (1 mentions)

4 protocols using isopropylthiogalactopyranoside iptg

Recombinant Protein Expression in E. coli

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E. coli strain
BL21(DE3) pLysS and the pET28a expression vector were obtained from
Novagen Inc. (Madison, WI). Amicon centrifugal filter devices (3000 M_r cutoff) were purchased from Millipore (Bedford,
MA). Q-Sepharose FF, SP-Sepharose FF, Superdex-200, and His-Trap HP
columns were obtained from Amersham Biosciences. Kanamycin, isopropyl
thiogalactopyranoside (IPTG), and 12-O-tetradecanoylphorbol-13-acetate
(PMA) were obtained from Sigma. Human α-thrombin (IIa), FXIa, pKLK, and plasmin were purchased from Haematologic Technologies
Inc. tPA (Alteplase) was purchased from Genentech (South San Francisco,
CA). uPA was obtained from Calbiochem, EMD Biosciences Inc. (San Diego,
CA). Glu–Plg was obtained from Enzyme Research Laboratories
(South Bend, IN). εACA (Amicar) was obtained from ICN Biomedicals
Inc. (Aurora, OH). Normal pooled plasma (NPP) was purchased from George
King Bio-Medical Inc. (Overland Park, KS). Plasmin substrate S-2251
(H-d-Val-Leu-Lys-p-nitroanilide) and pKLK and FXIa substrate S-2366 (pyroGlu-Pro-Arg-p-nitroanilide) were obtained from Diapharma Inc. All other
reagents were of the highest purity commercially available.

Kumar Y., Vadivel K., Schmidt A.E., Ogueli G.I., Ponnuraj S.M., Rannulu N., Loo J.A., Bajaj M.S, & Bajaj S.P. (2014). Decoy Plasminogen Receptor Containing a Selective Kunitz-Inhibitory Domain. Biochemistry, 53(3), 505-517.

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Statin Compounds Characterization Protocol

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Atorvastatin sodium salt (>98%), pravastatin sodium salt (≥98%), fluvastatin sodium salt hydrate (≥98%) and simvastatin (≥98%) were purchased from Cayman Chemical (Ann Arbor, MI, USA). Rosuvastatin sodium salt (98%) was synthesized by Synthex Technologies Sp. z o. o. according to previously reported procedure [40 (link)]. Potassium phosphate dibasic (K₂HPO₄) (≥98%) and potassium phosphate monobasic (KH₂PO₄) (≥98%) were purchased from J.T. Baker (New Jersey, NY, USA). Disodium edetate dihydrate (EDTA) (>98.5%), 4-(2-Hydroxyethyl)piperazin-1-ylethanesulfonic acid (HEPES) (99%), ethanol (99.8%), perchloric acid (HClO₄) (70%), magnesium chloride hexahydrate (MgCl₂ × 6H₂O) (99%) and potassium hydroxide (KOH) were purchased from Avantor Performance Materials (Gliwice, Poland). Adenosine 5′-monophosphate disodium salt (AMP) (≥99.9%), adenosine 5′-diphosphate sodium salt (ADP) (≥95%), adenosine 5′-triphosphate disodium salt hydrate (ATP) (99%), tetrabutylammonium hydrogensulfate (TBA) (97%), methanol (≥99.9%), isopropyl thiogalactopyranoside (IPTG), bacterial cell culture reagents and supplements for adenylate kinases production, BL21-CodonPlus (DE3)-RIL strain and pET-3a(+) expression vector were purchased from Sigma-Aldrich (Poznań, Poland).

Wujak M., Kozakiewicz A., Ciarkowska A., Loch J.I., Barwiolek M., Sokolowska Z., Budny M, & Wojtczak A. (2021). Assessing the Interactions of Statins with Human Adenylate Kinase Isoenzyme 1: Fluorescence and Enzyme Kinetic Studies. International Journal of Molecular Sciences, 22(11), 5541.

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Synthesis and Purification of Gold-Binding Proteins

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Polyethylene oxide (PEO, powder, molecular weight ≈ 200 000 g mol⁻¹) and gold nanoparticle solution (analytical grade, average particle size ≈10 nm) were purchased from Sigma–Aldrich (Poole, UK). All reagents were used without further purification. Isopropylthiogalactopyranoside (IPTG) was purchased from Sigma–Aldrich (Milwaukee, WI, USA). Amylose resin for column chromatography was purchased from New England Biolabs (Ipswich, MA, USA). The Instant Blue coomassie based staining solution was procured from Expedeon Inc. (San Diego, CA, USA). All buffers were filtered and degassed before using. Details on expression and purification of red fluorescent gold‐binding fusion proteins are given in Supporting Information and the genetic construction of the plasmid was described in our previous work.16

Zhang S., Karaca B.T., VanOosten S.K., Yuca E., Mahalingam S., Edirisinghe M, & Tamerler C. (2015). Coupling Infusion and Gyration for the Nanoscale Assembly of Functional Polymer Nanofibers Integrated with Genetically Engineered Proteins. Macromolecular Rapid Communications, 36(14), 1322-1328.

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Recombinant GST-Caveolin-1 Fusion Proteins

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The full length caveolin-1 (cav-1, amino acids 1-178) and its deletion mutants (amino acids1-101 and amino acids 82-101) were fused with Glutathione S-Transferase (GST). Along with a GST only negative control, all the GST-constructs were a kind gift from the laboratories of D. Volonte and F. Galbiati of the University of Pittsburgh School of Medicine. All these constructs were transformed into E Coli BL-21 strain and selected for ampicillin resistance. Logarithmically growing cells were induced for recombinant protein expression through the addition of 5mM isopropyl thiogalactopyranoside (IPTG, Sigma-Aldrich). All the GST-cav-1 constructs were affinity purified by passing through glutathione (GSH)-agarose beads using the detergent sarcosyl for initial solubilization.
Purified GST alone ( negative control), GST-cav-1 (full length 1-178), GST-cav-1 mutant

Dorai T., Shah A., Summers F., Mathew R., Huang J., Hsieh T.C, & Wu J.M. (2018). NRH:quinone oxidoreductase 2 (NQO2) and glutaminase (GLS) both play a role in large extracellular vesicles (LEV) formation in preclinical LNCaP-C4-2B prostate cancer model of progressive metastasis. The Prostate, 78(15).

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