Rabbit anti phospho eif2a

Manufactured by Cell Signaling Technology

Sourced in United States

Rabbit anti-phospho-eIF2A is a primary antibody that specifically recognizes the phosphorylated form of the eIF2A protein. eIF2A is a translation initiation factor involved in the regulation of protein synthesis.

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Lab products found in correlation

Rabbit anti hsp60, by Abcam (1 mentions) Mouse anti grp78, by R&D Systems (1 mentions) Rabbit anti β actin, by Cell Signaling Technology (1 mentions) P erk, by Cell Signaling Technology (1 mentions) Doxycycline, by Merck Group (1 mentions) Hrp conjugated goat anti rabbit, by Advansta (1 mentions) Blebbistatin, by Merck Group (1 mentions) Biotin phenol, by Merck Group (1 mentions) Hrp conjugated goat anti mouse, by Advansta (1 mentions) Cultrex 3 d culture matrix laminin 1, by R&D Systems (1 mentions) Cf dye phalloidin conjugates, by Biotium (1 mentions) Rabbit anti eif2a, by Cell Signaling Technology (1 mentions) Rabbit anti v5, by Cell Signaling Technology (1 mentions) Rabbit cleaved caspase 3 antibody, by Cell Signaling Technology (1 mentions) Isopropyl β d 1 thiogalactopyranoside iptg, by Merck Group (1 mentions) Rabbit anti gapdh, by Cell Signaling Technology (1 mentions) 4 hydroxytamoxifen, by Merck Group (1 mentions)

2 protocols using rabbit anti phospho eif2a

Comprehensive Western Blotting Protocol

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Western blotting was conducted according to standard protocols. The antibodies used in this study were as follows: rabbit anti-WWOX (a kind gift of Dr. M Aldaz, MD Anderson Cancer Centre, Houston, TX, USA) and ab137726 antibody (abcam), rabbit anti-β-actin (no. 4970; Cell Signalling Technology, Danvers, MA, USA), rabbit anti-Hsp60 (abcam; ab46798), rabbit anti-cleaved (Asp175) caspase-3 (R&D Systems, Minneapolis, MN, USA; 269518), mouse caspase-2 (no. 2224; Cell Signalling Technology), rabbit caspase-7 (no. 9492; Cell Signalling Technology), rabbit anti-phospho-eIF2A (Cell Signalling Technology; 9721), mouse anti-GRP78 (474421; R&D Systems), mouse anti-JNK1/JNK2 252323, MAB2076 and rabbit AF1205 (phospho-JNK) (R&D Systems), rabbit anti-phospho (T183/Y185)-JNK (AF1205), rabbit IRE-1 (ab48187 and ab37073; abcam) and PERK (no. 3192; Cell Signalling Technology).

Janczar S., Nautiyal J., Xiao Y., Curry E., Sun M., Zanini E., Paige A.J, & Gabra H. (2017). WWOX sensitises ovarian cancer cells to paclitaxel via modulation of the ER stress response. Cell Death & Disease, 8(7), e2955-.

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Immunofluorescence and Immunoblotting Protocols

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Reagents for immunofluorescence staining: mouse anti-filamin (Millipore), rabbit anti-Annexin A2 (Cell Signaling), rabbit anti-S100A14 (Sigma), CF-dye phalloidin conjugates (Biotium).
Antibodies for immunoblotting: rabbit anti-V5 (Cell Signaling), rabbit anti-GAPDH (Cell Signaling), rabbit anti-phospho-EIF2a (Cell Signaling), rabbit anti-EIF2a (Cell Signaling), rabbit cleaved caspase-3 antibody (Cell Signaling), rabbit Esyt1 antibody (ABclonal), HRP-conjugated goat anti-rabbit (Advansta), and HRP-conjugated goat anti-mouse (Advansta). Other reagents include: recombinant basement membrane (rBM; BD Biosciences), Cultrex 3D Culture Matrix Laminin I (R&D systems), blebbistatin (Sigma), polyethylenimine (Polyplus Transfection), doxycycline (Sigma), Isopropyl β-d-1-thiogalactopyranoside (IPTG) (Sigma), 4hydroxytamoxifen (Sigma), Biotin-phenol (Sigma).

Kai F., Ou G., Tourdot R.W., Stashko C., Gaietta G., Swift M., Volkmann N., Long A.F., Han Y., Huang H.H., Northey J.J., Leidal A.M., Viasnoff V., Bryant D.M., Guo W., Wiita A.P., Guo M., Dumont S., Hanein D., Radhakrishnan R., & Weaver V.M. (2021). ECM dimensionality tunes actin tension to modulate the endoplasmic reticulum and spheroid phenotype.

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