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6 keto prostaglandin f1α eia

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6-keto prostaglandin F1α EIA is a laboratory assay kit used to quantify the levels of 6-keto prostaglandin F1α, a stable metabolite of prostacyclin (PGI2), in various biological samples. This enzyme immunoassay (EIA) provides a sensitive and specific method for the measurement of 6-keto prostaglandin F1α concentrations.

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Lab products found in correlation

Cell invasion assay kit, by Merck Group (2 mentions) Horseradish peroxidase hrp conjugated anti mouse or anti rabbit igg antibodies, by Bio-Rad (2 mentions) Mouse β actin, by Merck Group (2 mentions) Immunocruz ip wb optima f system, by Santa Cruz Biotechnology (2 mentions) Anti txas antibodies, by Cayman Chemical (2 mentions) Mouse anti cox 1 antibody, by Cayman Chemical (2 mentions) Thromboxane b2 eia kit, by Cayman Chemical (2 mentions) Cox 1 and cox 2 primers, by Thermo Fisher Scientific (2 mentions) Polyclonal nitrotyrosine antibody clone 409 and other chemicals, by Merck Group (2 mentions) Growth factor reduced matrigel, by BD (2 mentions) Polyclonal pgi2 synthase antibody for ip, by ProSci (2 mentions)

Close spelling variants of this product

6-keto prostaglandin F1α (6 citations) Prostaglandins (6 citations) 6-keto Prostaglandin F1α EIA Kit (4 citations)

3 protocols using 6 keto prostaglandin f1α eia

1

Prostanoid Signaling Pathway Regulation

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Rabbit anti PGIS (1:500), anti-COX-2 (1:250) and anti-TXAS antibodies (1:250), mouse anti-COX-1 Antibody (1:250), 6-keto prostaglandin F1α EIA and thromboxane B2 EIA Kits were from Cayman Chemical Company (Ann Arbor, MI). PGIS siRNA (bovine), TXAS siRNA (bovine), non-silencing siRNA (NS-siRNA) and Immunocruz IP/WB Optima F System were obtained from Santa Cruz biotechnology, Inc. (Santa Cruz, CA). Growth factor-reduced Matrigel was from BD Biosciences (Bedford, MA). Cell invasion assay kit was obtained from Chemicon International (Temecula, CA). PGIS, TXAS, COX-1 and COX-2 primers were obtained from Invitrogen (Carlsbad, CA). Mouse β-actin (1:1,000) and polyclonal nitrotyrosine antibody (Clone 409) and other chemicals were obtained from Sigma Aldrich (St. Louis, MO). Polyclonal PGI2 synthase antibody for IP was obtained from ProSci Inc. (San Diego, CA). Horseradish peroxidase (HRP)-conjugated anti-mouse or anti-rabbit IgG antibodies (1:10,000) were obtained from Bio-Rad (Hercules, CA).
Mahajan C.N., Afolayan A.J., Eis A., Teng R.J, & Konduri G.G. (2014). Altered Prostanoid Metabolism Contributes to Impaired Angiogenesis in Persistent Pulmonary Hypertension of the Newborn. Pediatric research, 77(3), 455-462.
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2

Urinary 6-keto-Prostaglandin F1α Quantification

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Urinary 6‐keto‐prostaglandin F1α (6‐keto‐PGF1α, a stable prostacyclin metabolite) concentration was analyzed using an ELISA kit (6‐keto‐prostaglandin‐F1α EIA, Cayman Chemical Co) that has undergone analytic validation by the manufacturer and has been used previously in dogs.29 Using a similar technique as described with urinary 11‐dTXB2, urinary 6‐keto‐PGF1α was analyzed according to the manufacturer's instructions. Samples were analyzed in triplicate, averaged, and reported in picograms per milliliter of urine. Urine creatinine concentration was measured to calculate a urinary 6‐keto‐PGF1α‐to‐creatinine ratio.
Thomason J.M., Archer T.M., Wills R.W, & Mackin A.J. (2018). Effects of immunosuppressive agents on the hemostatic system in normal dogs. Journal of Veterinary Internal Medicine, 32(4), 1325-1333.
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3

Prostanoid Signaling Pathway Regulation

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Rabbit anti PGIS (1:500), anti-COX-2 (1:250) and anti-TXAS antibodies (1:250), mouse anti-COX-1 Antibody (1:250), 6-keto prostaglandin F1α EIA and thromboxane B2 EIA Kits were from Cayman Chemical Company (Ann Arbor, MI). PGIS siRNA (bovine), TXAS siRNA (bovine), non-silencing siRNA (NS-siRNA) and Immunocruz IP/WB Optima F System were obtained from Santa Cruz biotechnology, Inc. (Santa Cruz, CA). Growth factor-reduced Matrigel was from BD Biosciences (Bedford, MA). Cell invasion assay kit was obtained from Chemicon International (Temecula, CA). PGIS, TXAS, COX-1 and COX-2 primers were obtained from Invitrogen (Carlsbad, CA). Mouse β-actin (1:1,000) and polyclonal nitrotyrosine antibody (Clone 409) and other chemicals were obtained from Sigma Aldrich (St. Louis, MO). Polyclonal PGI2 synthase antibody for IP was obtained from ProSci Inc. (San Diego, CA). Horseradish peroxidase (HRP)-conjugated anti-mouse or anti-rabbit IgG antibodies (1:10,000) were obtained from Bio-Rad (Hercules, CA).
Mahajan C.N., Afolayan A.J., Eis A., Teng R.J, & Konduri G.G. (2014). Altered Prostanoid Metabolism Contributes to Impaired Angiogenesis in Persistent Pulmonary Hypertension of the Newborn. Pediatric research, 77(3), 455-462.
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