Gcms 5988 a

All melting points were determined on a Gallenkamp apparatus. Solvents were generally distilled and dried by standard literature procedures prior to use. The IR spectra were measured on a Pye-Unicam SP300 instrument in potassium bromide discs. The ¹H NMR spectra were recorded on a Varian Mercury VXR-300 MHz spectrometer and the chemical shifts δ down field from tetramethylsilane (TMS) as an internal standard. The mass spectra were recorded on a GCMS-Q1000-EX Shimadzu and GCMS 5988-A HP spectrometers, the ionizing voltage was 70 eV. Electronic absorption spectra were recorded on Perkin–Elmer Lambada 40 spectrophotometer. Elemental analyses were carried out by the Microanalytical Center of Cairo University, Giza, Egypt. Both diethyl chloromalonate and potassium chloromalonate were prepared as previously described [18] . 5-Amino-1H-pyrazole 1A, 3-amino[1,2,4]triazole 1E and 2-aminobenzimidazole 1F were purchased from Sigma Aldrich. 5-Amino-3-aryl-1H-pyrazoles 1B, 5-amino-3-(2-naphthyl)-1H-pyrazole 1C and 5-amino-3-(coumarin-3-yl)-1H-pyrazole 1D were prepared by literature procedures [19] , [20] .

All melting points (MPs) were measured on an Electrothermal Gallenkamp apparatus (Weiss Technik, London, UK). Infrared (IR) spectra were recorded in potassium bromide disks on SP3300 (Pye Unicam, Cambridge, England) and 8101 PC (Shimadzu, Tokyo, Japan) IR spectrophotometers. ¹H nuclear magnetic resonance (NMR) and ¹³C NMR spectra were recorded on a Varian Mercury VXR-300 spectrometer (300 MHz). Mass spectra were recorded on Shimadzu GCMS-Q1000-EX and GCMS 5988-A HP spectrometers, and ionizing voltage was 70 eV. Elemental analyses were carried out at the Microanalytical Center of Cairo University, Giza, Egypt. Biological evaluation of the products was carried out in the Microbiology Division of the Microanalytical Center of Cairo University.