Wrn 4666s

Manufactured by Cell Signaling Technology

Sourced in United States

WRN (4666S) is a primary antibody product developed by Cell Signaling Technology. It is designed to detect the Werner syndrome ATP-dependent helicase (WRN) protein. The WRN protein is a member of the RecQ helicase family and plays a role in DNA repair and telomere maintenance.

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Lab products found in correlation

Usp28 17707 1 ap, by Proteintech (2 mentions) Recql1 a300 450a, by Fortis Life Sciences (2 mentions) Blm a300 110a, by Fortis Life Sciences (2 mentions) Flag f3165, by Merck Group (1 mentions) Ub sc 8017, by Santa Cruz Biotechnology (1 mentions) Phospho chk1 ser345 2348, by Cell Signaling Technology (1 mentions) γh2ax 05 636, by Merck Group (1 mentions) C myc sc 40, by Santa Cruz Biotechnology (1 mentions) Gapdh 60004 1 1g, by Proteintech (1 mentions)

2 protocols using wrn 4666s

Antibody Characterization for Helicase Enzymes

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The antibodies used in this study were as follows: USP28 (17707-1-AP, 1:1000 WB, Proteintech); RECQL1 (A300-450A, 1:1000 WB, Bethyl Lab, Montgomery); WRN (4666S,1:1000 WB,Cell Signaling); BLM (A300-110A,1:1000 WB, Bethyl Lab); RECQL4 (17008-1-AP,1:1000 WB, Proteintech); RECQL5 (A302-520A, 1:2000 WB, Bethyl Lab); Flag (F3165, 1:2000, Sigma); Ub (SC-8017, 1:200 WB, Santa Cruz Biotechnology); γH2AX (05-636, 1:500 IF, Millipore); Claspin (2800S, 1:1000 WB, Cell Signaling); c-MYC (SC-40, 1:200 WB, Santa Cruz); Phospho-Chk1-Ser345 (2348, 1:1000 WB, Cell Signaling); CHK1 (ab32531, 1:1000 WB, Abcam); GAPDH (60004-1-1g, 1:5000 WB, Proteintech); Tubulin (66240-1-1g, 1:5000 WB, Proteintech); ACTIN (66009-1-1g, 1:3000 WB, Proteintech). The secondary antibodies conjugated to horseradish peroxidase for Western blot and the secondary antibodies anti-mouse, -goat or -rabbit Alexa fluor 488 or 594 for immunofluorescence staining were purchased from Jackson ImmunoResearch Laboratories. siRNAs were synthesized by GenePharma.

Wang J., Dong Y., Ma H., Wu L., Zhen X., Tang L., Jin J., Han S., Zhang P, & Peng J. (2021). The deubiquitinase USP28 stabilizes the expression of RecQ family helicases and maintains the viability of triple negative breast cancer cells. The Journal of Biological Chemistry, 298(1), 101443.

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Western Blot Antibodies for DNA Repair

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The antibodies used in this study were as follows: USP28 (17707-1-AP, 1:1000 WB, Proteintech, Wuhan, China); RECQL1 (A300-450A, 1:1000 WB, Bethyl Lab, Montgomery, TX, USA); WRN ( 4666S,1:1000 WB ,Cell Signaling, Beverly, MA, USA); BLM (A300-110A ,1:1000 WB, Bethyl Lab); RECQL4 (17008-1-AP ,1:1000 WB, Proteintech); RECQL5 (A302-520A, 1:2,000 WB, Bethyl Lab); Flag (F3165,

Peng J., Wang J., Dong Y., Ma H., Wu L., Zhen X., Tang L., Jin J., Han S., & Zhang P. (2021). The Deubiquitin Enzyme USP28 Maintains the Viability of Triple Negative Breast Cancer Cells through Stabilizing the RecQ Family Helicases.

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