Cells seeded into Ibitreat μ-dishes (Ibidi), simultaneously transfected with GFPNMHCIIA and mcherrySEC61, maintained in HBSS at 37°C with 5% CO2 were imaged using an Andor Revolution XD Spinning-disk confocal system with an EMCCD iXonEM+ camera, 488 nm lase lines, and a Yokogawa CSU-22 unit on an inverted microscope (IX81; Olympus), driven by Andor IQ live-cell imaging software. LLO (0.5 nM) was added 10 min after initial image acquisition. Differential interference contrast (DIC) images and GFP fluorescent data sets with 0.5 μm Z-steps were acquired using a UPLSAPO 100x/1.40 objective lens every 15 sec. ImajeJ64 was used for image sequence analysis and video assembly.
Csu 22
Manufactured by Olympus
The CSU-22 is a compact and versatile cell sorter unit designed for laboratory applications. It serves as a core component for cell analysis and sorting processes. The CSU-22 provides high-speed, multi-parameter cell sorting capabilities to support various research and diagnostic workflows.
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Lab products found in correlation
2 protocols using csu 22
1
Imaging Live Cell Interactions
2
Confocal Imaging of Larval Nervous System
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Nerve cords of staged larvae were dissected in Sørensen’s phosphate buffer (0.1 M [pH 7.2]), transferred onto a poly-L-lysine (Sigma) coated coverslip in saline, and imaged immediately with a Yokagawa CSU-22 confocal field scanner mounted on an Olympus BX51WI microscope, using a 63×/1.2 NA (Olympus) water immersion objective. Images were acquired at 0.3-μm z steps using a QuantEM cooled EMCCD camera (Photometrics), operated via MetaMorph software (Molecular Devices). Dye fills were carried out as detailed previously [31 (link)].
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