For miRNA and small interfering RNA (siRNA) transfections, 6 × 104 LX-2 cells were plated on a 24-well plate and incubated overnight under regular growth conditions. Next, miR-1914-5p mimic or inhibitor (mirVana™miRNAs, Thermo Fisher Scientific, USA) and MLYCD-siRNA and ACSL4-siRNA or their Non-targeting Pool (OnTarget Plus siRNA – Smart Pools – Dharmacon/GE Health Care, USA) were transfected into cells, according to Silva et al. (2016) (link). At 48 h after transfection, cells were collected for further investigation.
Mirvana mirnas
Manufactured by Thermo Fisher Scientific
Sourced in United States
The MirVana™miRNAs is a product line of reagents and kits designed for the isolation and purification of microRNAs (miRNAs) from various biological samples. The core function of the MirVana™miRNAs is to enable efficient extraction and recovery of miRNAs, which are small, non-coding RNA molecules involved in the regulation of gene expression.
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Lab products found in correlation
2 protocols using mirvana mirnas
1
miRNA and siRNA Transfection in LX-2 Cells
2
Enhancing Muscle Function in Muscular Dystrophy
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For gain- and loss-of-function analysis, mice were anesthetized by using isoflurane, 2% to 5% inhaled, to a surgical plane of anaesthesia. Twenty-four C57BL/6, 15 mdx, and eight DBA2J/mdx mice were injected in each in each TA muscle with 50 μL of cardiotoxin solution (CTX; 10 μM) together with 11.55 μg of mirVana miRNAs (Thermo). This dose is equivalent to 0.57 mg per kg body weight.33 (link) Negative Control #1 Inhibitor (4464077) was used as Scramble, hsa-miR-106b-5p mimic (4464067) was used as premiR-106b and hsa-miR-106b-5p inhibitor (4464085) was used as antimiR-106b. Finally, an exhaustion treadmill trial was performed to evaluate the endurance of treated 4-month-old male mdx mice (and C57BL/6 mice were used as controls) fusing a motorized treadmill (Panlab Treadmills) supplied with a shocker plate as described previously.34 (link) The treadmill was run at 0° of inclination at 5 m/min for 5 min; subsequently, the speed was increased 1 m/min every minute. The test ended when the mouse remained more than 20 s on the shocker plate.
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