L ascorbic acid sodium salt

Manufactured by Merck Group

Sourced in United States

L(+)-ascorbic acid sodium salt is a laboratory reagent that serves as a source of ascorbic acid, also known as vitamin C. It is a white to yellowish-white crystalline powder that is readily soluble in water. The product is commonly used in various scientific and analytical applications as a reducing agent, antioxidant, and nutrient supplement.

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8 protocols using l ascorbic acid sodium salt

Ascorbic Acid Solution Application

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A 10% solution of SA was prepared by dissolving 10 g of ascorbic acid powder (L (+) ascorbic acid sodium salt, Sigma-Aldrich, St. Louis, MO, USA) in 100 mL of distilled water. Groups G3, G5, G7 and G9 were treated with this solution as follows: 10 mL of 10% SA solution (pH = 7.4) was applied onto the vestibular surface with a syringe, with a flow rate of 1 mL per minute, for 10 min in order to keep the surface wet. The teeth were then rinsed with distilled water for 30 S and dried using compressed air for 5 S to obtain a mat surface, prior to the bonding procedure.

Ghaleb M., Orsini G., Putignano A., Dabbagh S., Haber G, & Hardan L. (2020). The Effect of Different Bleaching Protocols, Used with and without Sodium Ascorbate, on Bond Strength between Composite and Enamel. Materials, 13(12), 2710.

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Plasma Treatment of Aqueous Solutions

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β-Nicotinamide adenine dinucleotide reduced disodium salt hydrate (NADH), β-nicotinamide adenine dinucleotide sodium salt hydrate (NAD+), β-nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt hydrate (NADPH), 1,4-hydroquinone, 1,4-benzoquinone, and L(+)-ascorbic acid sodium salt (vitamin C sodium salt) were purchased from Sigma-Aldrich (USA). H₂O₂, nitrate, and nitrite test strips were purchased from Bartovation LLC (New York, NY, USA). These strips were used for the detection of H₂O₂, NO₂⁻, and NO₃⁻ in water after the treatment with cold atmospheric pressure He-plasma jet. Time of the aqueous solution treatment was shown in Figure 3. Bottled ultra-high purity helium was purchased from Sexton Welding Supply (Huntsville, AL, USA).

Volkov A.G., Hairston J.S., Taengwa G., Roberts J., Liburd L, & Patel D. (2022). Redox Reactions of Biologically Active Molecules upon Cold Atmospheric Pressure Plasma Treatment of Aqueous Solutions. Molecules, 27(20), 7051.

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Preparation of Cy5-Labeled Peptide p41

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Peptide p41 (SWLRRIWRWICKVLSRFK) and Cy5-labeled p41 were custom synthesized by AnaSpec (USA). α-(9-Fluorenylmethyloxycarbonyl)amino-ω-carboxy succinimidyl ester poly(ethylene glycol) (Fmoc-PEG-NHS, M_W (PEG) = 5000 g mol⁻¹, M_w/M_n = 1.02) was purchased from Jenkem Technology (China). Amberlyst^® 15 hydrogen form, molecular sieve UOP type 3 Å, L-glutamic acid γ-benzyl ester, D (+)-galactose, propargylamine, L(+)-ascorbic acid sodium salt and copper (II) sulfate pentahydrate, 98+%, A.C.S. reagent were obtained from Sigma-Aldrich (U.S.). Silica gel (for chromatography, 0.03–0.200 mm, 60 Å), 2-bromoethanol, ethyl acetate, methanol, dichloromethane (DCM), dimethylformamide (DMF), tetrahydrofuran (THF) were purchased from Acros Organics (USA). S-2-(4-Isothiocyanatobenzyl)-diethylenetriamine pentaacetic acid (SCN-DTPA) was ordered from Macrocyclics (Dallas, TX, USA). Lutetium-177 trichloride was obtained from PerkinElmer (USA).

Zhang J., Garrison J.C., Poluektova L.Y., Bronich T.K, & Osna N.A. (2015). Liver-targeted antiviral peptide nanocomplexes as potential anti-HCV therapeutics. Biomaterials, 70, 37-47.

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Polycaprolactone Gelatin Antimicrobial Scaffold

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Autoclaved phosphate-buffered saline (1× PBS) was used for all in vitro experiments. PCL (M_n = 80,000), gelatin from bovine skin, DAPI, hydrochloric acid, sodium nitrite, copper(II) chloride, l-ascorbic acid sodium salt, acetone, Cell Counting Kit-8 (CCK-8), and 1,1,1,3,3,3-hexafluoroisopropanol (HFIP) were purchased from Sigma-Aldrich (St. Louis, MO). l-Glutathione (reduced 98+%) was obtained from Alfa Aesar (Ward Hill, MA). Alexa Fluor 488 Phalloidin was purchased from Thermo Fisher Scientific (Waltham, MA). Dulbecco’s modified Eagle’s medium (DMEM) and trypsin–EDTA were purchased from Corning (Manassas, VA). The antibiotic penicillin–streptomycin (Pen–Strep) and fetal bovine serum were purchased from Gibco-Life Technologies (Grand Island NY 14072). The mouse fibroblast cell line (ATCC 1658) and Staphylococcus aureus (ATCC 5538) were originally obtained from American Tissue Culture Collection (ATCC).

Hopkins S.P., Pant J., Goudie M.J., Nguyen D.T, & Handa H. (2020). Electrospun Bioabsorbable Fibers Containing S-Nitrosoglutathione for Tissue Engineering Applications. ACS applied bio materials, 3(11), 7677-7686.

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Photosynthetic Protein Immobilization

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Rink-amide AM resin (200–400 mesh) was purchased from Merck Biosciences (Darmstadt, Germany). N-(9-fluorenylmethoxycarbonyl) (Fmoc)-protected-amino acids, 1-hydroxybenzotriazole (HOBT), 2-(1H-benzotriazole-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate (HBTU), N,N-diisopropylethylamine (DIEA), piperidine, trifluoroacetic acid (TFA), and N-methyl pyrrolidone (NMP) were purchased from Watanabe Chemical Industries (Hiroshima, Japan). Acrylamide and VA-057 were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Poly(ethylene glycol) average M_n = 1500 g/mol (PEG1500), n-dodecyl-β-d-maltopyranoside (β-DDM), dichloroindophenol (DCIP), L(+)-Ascorbic Acid Sodium Salt, and methyl viologen dichloride dihydrate (MV²⁺) were purchased from Sigma-Aldrich (St. Louis, MO, USA). The RAFT initiator containing the dithiopyridyl (DTP) group, BSTP pyridyl disulfide, was synthesized according to previous study [27 (link)]. PSI derived from T. vulcanus was prepared similar to the previous study [21 (link)]. Unless otherwise stated, other chemicals and reagents were obtained commercially and used without further purification. BSTP disulfide was synthesized as following the previous study.

Shimamoto T., Nakakubo T., Noji T., Koeda S., Kawakami K., Kamiya N, & Mizuno T. (2021). Design of PG-Surfactants Bearing Polyacrylamide Polymer Chain to Solubilize Membrane Proteins in a Surfactant-Free Buffer. International Journal of Molecular Sciences, 22(4), 1524.

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Carbon Fiber Electrochemical Sensors

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Carbon fibers (∼5 μm
in diameter) were obtained from World Precision Instruments. Silver
conductive epoxy was purchased from MG Chemicals. Five-minute nonconductive
epoxy was obtained from Devcon. l-ascorbic acid sodium salt,
dopamine hydrochloride, norepinephrine hydrochloride, tricaine (ethyl
3-aminobenzoate methanesulfonate salt), desipramine hydrochloride,
and agar were purchased from Sigma-Aldrich. Calcium chloride was obtained
from Acros Organics. 2-Propanol, sodium hydroxide, sodium chloride,
and magnesium sulfate were purchased from Fisher Scientific. Sodium
phosphate dibasic was purchased from Spectrum. Potassium phosphate
monobasic and potassium chloride were purchased from LabChem, Inc.
Serotonin hydrochloride was purchased from Alfa Aesar. Epinephrine
hydrochloride was obtained from MP Biomedicals. Dimethylsulfoxide
(DMSO) was purchased from J. T. Baker. Nomifensine maleate salt was
obtained from ChemCruz. 0.1 M phosphate buffer (PB), pH 7.5, was prepared
by mixing sodium phosphate dibasic and potassium phosphate monobasic.
E3 medium (pH 6.9–7.2) containing 5 mM sodium chloride, 0.17
mM potassium chloride, 0.33 mM magnesium sulfate, and 0.33 mM calcium
chloride was prepared in deionized water. All the solutions were prepared
using purified water (18 MΩ, Millipore, Direct-Q System).

Dumitrescu E., Deshpande A., Wallace K.N, & Andreescu S. (2022). Time-Dependent Monitoring of Dopamine in the Brain of Live Embryonic Zebrafish Using Electrochemically Pretreated Carbon Fiber Microelectrodes. ACS Measurement Science Au, 2(3), 261-270.

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Purifying Recombinant FIH, ASPP2, and iASPP

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Recombinant forms of FIH (full length), ASPP2_889-₁₁₂₈-Avi-His₆, and iASPP_625-₈₂₈-Avi-His₆, were produced and purified as described (8 (link), 34 (link)). Peptides (all with C-terminal amides) were from GL Biochem. l-(+)-ascorbic acid sodium salt (code: 11140), ammonium iron (II) sulfate hexahydrate (code: 215406), N-oxalylglycine, and 2OG disodium salt hydrate (K3752) were from Sigma–Aldrich.

Leissing T.M., Hardy A.P., Chan H., Wang Y., Tumber A., Chowdhury R., Feng T., Coleman M.L., Cockman M.E., Kramer H.B., Berridge G., Fischer R., Kessler B.M., Ratcliffe P.J., Lu X, & Schofield C.J. (2022). Factor inhibiting HIF can catalyze two asparaginyl hydroxylations in VNVN motifs of ankyrin fold proteins. The Journal of Biological Chemistry, 298(6), 102020.

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In Vitro Generation of FRC-Derived Matrices

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FRC-derived matrices were generated in vitro according to published methods (Franco-Barraza et al., 2016 (link)). In brief, gelatin-coated wells were used to culture FRC cell lines at 5x10³ cells/cm² in culture media supplemented with 50 μg/ml L(+)-Ascorbic acid sodium salt (Sigma-Aldrich) for 5 days, unless otherwise stated. Supplemented media was replenished at day 1 and 3. For proteomic analysis, cells in their matrix were collected in PBS, centrifuged and resuspended in 4M Urea. For microscopy analysis, cells were lysed incubating for 15 min at 37°C in PBS 1% Triton X-100 20 mM ammonium hydroxide. Matrices were blocked with 2% bovine serum albumin (w/v) (Sigma-Aldrich) and stained with the indicated antibodies. Samples were imaged with a Leica TCS SP5 Confocal Microscope using 63X oil HCX PL APO lenses.

Martinez V.G., Pankova V., Krasny L., Singh T., Makris S., White I.J., Benjamin A.C., Dertschnig S., Horsnell H.L., Kriston-Vizi J., Burden J.J., Huang P.H., Tape C.J, & Acton S.E. (2019). Fibroblastic Reticular Cells Control Conduit Matrix Deposition during Lymph Node Expansion. Cell Reports, 29(9), 2810-2822.e5.

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