Kidneys were isolated at the appropriate stage and fixed in 4% PFA for 1 hour. Cryosections were immunostained as previously described [28 (link)]. Antibodies used include Six2 (Proteintech, 11562-1-AP), FLAG (Sigma, F1804), Wt1 (Abcam, ab89901), pan-cytokeratin (Sigma, C2931), Pax8 (Abcam, ab13611), Ecad (Sigma, U3254), Six3 (Rockland, 200-201-A26S), and LTL-FITC (Vector Labs, FL-1321). Images were acquired on a Nikon Eclipse 90i epi-fluorescent microscope or Zeiss LSM 780 inverted confocal microscope.
Ltl fitc
Manufactured by Vector Laboratories
LTL-FITC is a fluorescent label produced by Vector Laboratories. It is used for the detection and visualization of target proteins or molecules in various biological applications.
Automatically generated - may contain errors
Lab products found in correlation
Lsm 780, by Zeiss (2 mentions)
Eclipse 90i, by Zeiss (1 mentions)
Pan cytokeratin, by Merck Group (1 mentions)
Flag tag (flag), by Merck Group (1 mentions)
Lysis buffer, by Merck Group (1 mentions)
Edta vacutainer tube, by BD (1 mentions)
Sna fitc, by Vector Laboratories (1 mentions)
Mal 2 fitc, by Vector Laboratories (1 mentions)
Sp 5040, by Vector Laboratories (1 mentions)
R7757, by Merck Group (1 mentions)
2 protocols using ltl fitc
1
Kidney Immunohistochemistry Protocol
2
CTC Analysis by Flow Cytometry
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The protocol of flow cytometry for CTC analysis was previously established (4 (link)) and optimized for comprehensive analysis of glycans and other protein markers. The blood samples were collected into EDTA Vacutainer tubes (Becton Dickinson) from patients with breast cancer and stored on ice or at 4°C temporarily prior to manual blood processing within 24 hours. After multiple rounds (two to four rounds) of red blood cell lysis (lysis buffer, Sigma, R7757), white blood cells (mainly peripheral blood mononuclear cells) were washed twice with PBS. Cells were fixed with 3% paraformaldehyde for 30 minutes for glycan and protein profiling analyses. Cells were blocked with carbo-free blocking solution (Vector Laboratories, SP-5040) and stained with antibodies for proteins (CD45, epithelial marker EpCAM, and other blood cell lineage markers) and lectins for glycans, such as SNA-FITC, MALII-FITC, PHA-L-PE, LTL-FITC, RCA-Rho, LEL-APC, ConA-PE (Vector Laboratories), and MALII-FITC (GlycoMATRIX). CTCs were gated based on cell size (forward-scatter and side-scatter channels), CD45 negativity, and EpCAM+/− CK+/−.
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