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Measurescope mm 11

Manufactured by Nikon
Sourced in Japan

The Nikon Measurescope MM-11 is a precision microscope designed for measurement and inspection applications. It features high-quality optics, a stable and accurate stage, and a range of accessories to support various measurement tasks. The core function of the Measurescope MM-11 is to provide precise and reliable measurements of small objects or features.

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7 protocols using measurescope mm 11

1

Interfacial Gap Analysis of Resin Composites

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Immediately after setting (i.e., at 3 min after the start of light activation) or after storage in distilled water at 37°C for one day, excess composite restorative material on each restoration surface was removed by wet grinding on #600 carborundum paper. This was followed by polishing with linen and an aqueous alumina slurry (Alpha Micropolish, Buehler, Chicago, IL, USA). Then, distilled water was used for rinsing to avoid desiccation and erosion.
Each tooth was sectioned in a buccolingual direction through the center of the restoration with a low-speed diamond saw (IsoMet, Buehler, Lake Bluff, IL, USA). The presence or absence of interfacial gaps [14 (link)] was inspected and measured at 14 points, labelled as Point #1 to Point #14, each 0.5 mm apart (Fig 1), along the interface between the restoration and cavity walls (n = 10; total points measured per resin composite = 140). Inspection and measurements were carried out using a measurement microscope at ×1,000 magnification (Measurescope MM-11, Nikon, Tokyo, Japan), with the cavity walls and cavity floor of each half of the sectioned tooth facing up. At 3 min after the start of light activation or after one-day storage, the number of gaps at each measurement point (Point #1 to Point #14) in each tooth was summed up for each resin composite restorative material [14 (link), 15 (link)].
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2

Fracture Surface Analysis of Dental Bonds

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The fractured surface of each post µTBS test specimen was observed under an optical microscope (Measurescope MM-11; Nikon, Tokyo, Japan) at 200× magnification. In addition, the typical specimen in each experimental condition was observed to confirm the fracture mode composition and the structure of each fractured surface using a scanning electron microscope (SEM; JSE-IT200; Hitachi, Tokyo, Japan) with an accelerating voltage of 5.0 kV after mounting to specimen stub using conductive tape and osmium coating (approximate 10 nm thickness) on the abutment-side fractured surface.
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3

Evaluating Resin Composite Restoration Margins

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One cavity was prepared in each of 180 teeth (9 resin composite restorative materials × 2 polishing times × 10 replicates), using the same, standardized cavity dimensions and preparation and restoration procedures described in subsection 2.3 for Class I cavities. Immediately after light-curing, excess composite restorative material on each restoration surface was removed by wet grinding on #600 carborundum paper. This was followed by polishing with linen and an aqueous alumina slurry (Alpha Micropolish, Buehler, Chicago, IL, USA).
Cavosurface margin on the enamel surface of each restoration (Fig 2) was inspected using a measurement microscope at ×1,000 magnification (Measurescope MM-11, Nikon, Tokyo, Japan). Maximum gap-width and opposing width (if any) between the restorative material and enamel margin was recorded [11 (link), 12 (link)]. Maximum gap-widths in each tooth (n = 10) were summed up for each resin composite restorative material.
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4

Fracture Mode Analysis of Restorations

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The fracture mode of each post-test specimen was observed by using an optical microscope (Measurescope MM-11, Nikon, Tokyo, Japan) at a 200× magnification. Furthermore, to confirm the composition of the fractured surface, the dentin-side surface of the representative specimen selected from each restoration group was osmium-coated and then observed using a scanning electron microscope (SEM, JSE-IT200, Hitachi, Tokyo, Japan) with an accelerating voltage of 10.0 kV.
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5

Analyzing Fracture Modes of Dental Specimens

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Following the µ-TBS test, the facture mode of each specimen was observed with a light microscope (Measurescope MM-11, Nikon) at ×200 magnification. In addition, typical post-test specimens were selected from all specimens using three restorative block materials set with the three space setting values. The dentin-side surface of every post-test specimen was osmium-coated and observed by a scanning electron microscope (SEM; S-4000, Hitachi, Tokyo, Japan) under ×50 magnification with an accelerating voltage of 5.0 kV, to confirm the fracture mode components. Prior to the SEM observation, fracture surfaces of the beam-shaped specimens made of the immediate dentin sealing material (Clearfil Majesty ES Flow) and the resin cement (PANAVIA V5) were prepared. Fabricated surfaces of the three CAD/ CAM restorative blocks were also prepared. Two types of "cohesive fracture" occurred within the immediate dentin sealing material and the resin cement and the intact fabricated surfaces of the CAD/CAM blocks were observed by SEM as an observation index.
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6

Fracture Mode Observation of Dental Restorations

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After the µ-TBS measurements, the fracture mode of each specimen was observed with a light microscope (Measurescope MM-11, Nikon, Tokyo, Japan) at a magnification of ×200.
In addition, nine post-test specimens were randomly selected from all specimens of three types of restoration luted with the three cement systems. The dentin-side surfaces of post-test specimens were osmium-coated and observed by a scanning electron microscope (SEM; S-4000, Hitachi, Tokyo, Japan) under ×50 magnification with an accelerating voltage of 5.0 kV.
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7

Fracture Mode Analysis of Dental Crowns

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After μ-TBS measurements, the fracture mode of each dumbbell-shaped specimen was observed using a light microscope (Measurescope MM-11, Nikon, Tokyo, Japan) at a magnification of 200×. Fracture mode was classified to Ri: interfacial fracture occurring at the interface between fabricated crown and resin cement; Cc: cohesive fracture occurring within the resin cement; Ai: interfacial fracture occurring at the interface between abutment and resin cement.
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