The analysis was performed using a Shimadzu Prominence LC–20A system (Shimadzu, Kyoto, Japan) consisting of a solvent delivery unit, an on-line degasser, a column oven, an autosampler, and a photodiode array (PDA) detector, was employed. The data processor used LCsolution software (version 1.24; Shimadzu, Kyoto, Japan). The 3,29-dibenzoyl-rarounitriol was separated on a Phenomenex Gemini C18 column (250 × 4.6 mm; particle size 5 μm, Phenomenex, Torrance, CA) and maintained at 40°C. The mobile phases for chromatographic separation were carried out using isocratic elution (A : B = 5 : 95) of solvent A (distilled water) and solvent B (acetonitrile) for 30 min. The analysis was performed at a flow-rate of 1.0 mL/min using a detection wavelength of 230 nm. The injection volume was 10 μL.
Lcsolution software version 1.24
Manufactured by Shimadzu
Sourced in Japan
LCsolution software (version 1.24) is a data processing and instrument control software for Shimadzu liquid chromatography (LC) systems. It provides essential functions for the operation and data analysis of LC instruments.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using lcsolution software version 1.24
1
Separation and Quantification of 3,29-dibenzoyl-rarounitriol
2
Quantifying Mefloquine and Verapamil in Feline Plasma
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Mefloquine and verapamil were quantified in feline plasma based on modified HPLC method [16 (link)]. The HPLC system consisted of a Shimadzu LC-20AT delivery unit, DGU-20A3 HT degassing solvent delivery unit, SIL-20A auto injector, SPD-20A UV detector and CTO-20A column oven. Shimadzu LC Solution software version 1.24 (Kyoto, Japan) was used for chromatographic control, data collection and data processing. Chromatographic separation was performed with a Microsorb-MV C18 column (250 mm × 4.6 mm i.d., 5.0 μm; Varian, Mulgrave, VIC, Australia) with a 1.0 mm Optic-guard C18 pre-column (Choice Analytical, Thornleigh, NSW, Australia) under a pressure of 1,900 psi at 25°C. The isocratic mobile phase consisted of a mixture of 25 mM sodium phosphate buffer with 1.0% triethylamine adjusted to pH 2.5 and acetonitrile and methanol (50:25:25, v/v/v) at a flow rate of 1.0 mL/min. The injection volume was 10 μL per sample. The diode array detector was set at a wavelength of 220 nm. The retention times of verapamil and mefloquine were approximately 8.5 and 14.5 minutes, respectively.
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