Horseradish peroxidase conjugated anti sheep goat igg from donkey

All chemical reagents were obtained from Sigma (St. Louis, MO, USA), except where noted. Western blotting detection reagents were obtained from Amersham (Bucks, UK). RNAzol^TM B was obtained from TEL‐TEST Inc (Friendwood, TX, USA). Antibodies against α‐tubulin (sc‐5286), β‐actin (sc‐47778), TFIIB (sc‐271736), Histone H1 (sc‐8615), p‐Akt (sc‐101629), total‐Akt (sc‐1618), p‐PERK (sc‐32577), PERK (sc‐13073), IRE (sc‐390960), ATF6 (sc‐22799), CHOP (sc‐575), PPARγ (sc‐7196), pS‐IRS (sc‐33956), pT‐IRS (sc‐17196), IRS (sc‐559) and SREBP‐1c (sc‐365513) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies against p‐IRE (ab48187), PPARα (ab24509), CPT1α (ab128568) and Acox1 (ab184032) were purchased from Abcam (Cambridge, UK). Antibodies against FoxO6 and p‐FoxO6 (Ser184) were obtained from Dr Dong (University of Pittsburgh, Pittsburgh, PA, USA). Horseradish peroxidase‐conjugated anti‐rabbit IgG and horseradish peroxidase‐conjugated anti‐mouse IgG antibodies were obtained from Amersham (Bucks, UK). Horseradish peroxidase‐conjugated anti‐sheep/goat IgG from donkey was purchased from Serotec (Oxford, UK). Polyvinylidene difluoride (PVDF) membranes were obtained from Millipore Corporation (Bedford, MA, USA). PPARα‐siRNA (20 nmol/L) was obtained from Integrated DNA Technologies (IDT; Coralville, Iowa).

We obtained all chemical reagents from Sigma (St. Louis, MO, USA), except as otherwise noted. The compound 2’,7’-dichlorodihydrofluorescein was obtained from Molecular Probes Inc. (Eugene, OR, USA). We obtained Western blotting detection reagents from Amersham (Bucks, UK); RNAzol B from TEL-TEST Inc. (Friendwood, TX, USA); antibodies against TXNIP, IL-1β, caspase-1, ASC, NLRP3, total-Akt, p-Akt, histone H1, β-actin, and α-tubulin from Santa Cruz Biotechnology (Dallas, TX, USA); antibodies against FoxO6 and p-FoxO6 (Ser184) from Dr. HH Dong (University of Pittsburgh, Pittsburgh, PA, USA); horseradish peroxidase-conjugated anti-rabbit immunoglobulin G (IgG), and horseradish peroxidase-conjugated antimouse IgG antibodies from Amersham; horseradish peroxidase-conjugated anti-sheep/goat IgG from donkey from Serotec (Oxford, UK); and polyvinylidene difluoride (PVDF) membranes from Millipore Corporation (Billerica, MA, USA).