Dnp specific mouse ige spe 7

All cell culture reagents and DNP-specific mouse IgE (SPE-7) were purchased from Invitrogen (Carlsbad, CA, USA). Recombinant murine interleukin-3 (IL-3), stem cell factor (SCF), and recombinant human SCF were purchased from Peprotech (Rocky Hill, NJ, USA). DNP-BSA and p-nitrophenyl-N-acetyl-β-D-glucosamine (PNAG) were from Sigma-Aldrich (St. Louis, MO, USA), Compound 48/80 was from AnaSpec (Fremont, CA, USA). Amaxa transfection kit (Kit V) was purchased from Lonza (Gaithersburg, MD, USA). PE anti-human MRGPRX2 antibody was purchased from Biolegend (San Diego, CA, USA). Polyclonal MRGPRX2 Ab was purchased from Novus Biologicals (Littleton, CO, USA). HRP-conjugated anti-rabbit IgG was from Cell Signaling Technologies (Danvers, MA, USA). West Pico Chemiluminescent Substrate was from Thermo Scientific (Rockford, IL, USA). DNeasy Blood and Tissue Kit was purchased from Qiagen (Germantown, MD, USA). QuikChange II Site-Directed Mutagenesis Kit was purchased from Agilent Genomics (Santa Clara, CA, USA). Plasmid encoding hemagglutinin (HA)-tagged human MRGPRX2 in pReceiver-MO6 vector was obtained from GeneCopoeia (Rockville, MD, USA). Antimicrobial peptidomimetics (compound 1, compound 2, compound 3, compound 4, and compound 5) were obtained from Fox Chase Chemical Diversity Center (Doylestown, PA, USA).

All cell culture reagents, 2,4-dinitrophenyalted Bovine Serum Albumin (DNP-BSA; Cat # A23018) and DNP-specific mouse IgE (SPE-7) were purchased from Invitrogen (Gaithersburg, MD, USA). Recombinant mouse interleukin-3 (IL-3) and stem cell factor (SCF) were purchased from Peprotech (Rocky Hill, NJ). 2-Mercaptoethanol (Cat #M7522), p-nitrophenyl-N-acetyl-β-D-glucosamine (PNAG) and Evans blue dye were obtained from Sigma-Aldrich (St. Louis, MO). Fura-2 acetoxymethyl ester was purchased from Abcam (Cambridge, MA, USA). Compound 48/80 was obtained from AnaSpec (Fremont, CA). Phycoerythrin (PE)- conjugated c-kit and Allophycocyanin (APC)-conjugated FcϵRI antibodies were acquired from eBiosciences (San Diego, CA) Paroxetine hydrochloride hemihydrate (CAS RN: 110429-35-1, Product Number: P1977, solvent PBS) was obtained from TCI chemicals, paroxetine analog CCG258747 (solvent DMSO) was kindly provided by Dr. John J. G. Tesmer, Purdue University.