Freshly collected tissues were fixed by 10% formalin and embedded in paraffin. For histopathology, cross-sections were prepared at 5 μm and stained with hematoxylin and eosin (HE). For immunohistochemistry (IHC) staining, a duplicate 4-μm section was cut, mounted on positively charged SuperFrost Plus microscope slides, dewaxed, and rehydrated. Antigen retrieval was performed by pressure-cooking for 25 mins in pH 6 citrate buffer. Endogenous peroxidases were blocked using 3% H2O2 for 15 mins. Non-specific bindings were blocked using normal goat serum at 1:10 in TBS for 15 mins. Slides were incubated overnight at 4°C with primary rabbit anti-influenza A nucleoprotein (NP) polyclonal antibody (Catalog ID19382; LifeSpan Biosciences, WA) at 1:50 dilution. The EnVision AP (DAKO K1396, Carpinteria, CA) detection system and nuclear fast red (DAKO K1396) were used as chromogens. Sections were counterstained with Mayer’s hematoxylin. Positive and negative control sections were included in each IHC run. All tissues were systematically screened for microscopic lesions. The intensity of viral antigen staining in each section was also scored as following: (-), no antigen staining; (+), infrequent; (++), common; and (+++), widespread staining.
Envision ap
Manufactured by Agilent Technologies
Sourced in United States
The EnVision AP is a versatile lab equipment designed for spectrophotometric analysis. It is capable of performing a wide range of absorbance-based measurements, including ELISA, fluorescence, and luminescence assays. The core function of the EnVision AP is to provide accurate and reliable data for various analytical applications in research and development laboratories.
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Lab products found in correlation
2 protocols using envision ap
1
Immunohistochemical Detection of Influenza A
2
Immunohistochemical Analysis of Tenascin-C
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Tissue specimens were postfixed in 4% paraformaldehyde overnight and stored at 4°C in 30% sucrose prior to cryosectioning. For immunohistochemistry, 5-μm sections were cut and examined for Tenascin-C expression using immunohistochemistry. Antigen retrieval was carried out using an autoclave for 10 min in 10 mmol/L sodium citrate buffer. Tenascin-C was stained with rabbit polyclonal anti-Tenascin-C antibody (dilution, 1:100; Santa Cruz Biotechnology Inc., Santa Cruz, California, USA). Bound antibodies were detected using Envision/AP (DAKO, Carpinteria, California, USA) followed by counterstaining with hematoxylin.
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