Shortpass filter

A Samsung Galaxy Note 4 smartphone was purchased for the imaging of
the RT-LAMP reaction on the microchip substrate. The smartphone hardware was
not modified from its factory conditions. A Thorlabs 530 nm Longpass Colored
Glass Filter was placed between the camera and the chip to isolate the
fluorophore emission wavelengths. A 3D-printed cradle (Figure 1) was designed to position the smart-phone
horizontally with the camera directly above the microchip. A mounting
cylinder was also 3D printed to hold an Opto Diode Corp high-output blue
light-emitting diode (LED) and a Thorlabs Shortpass Filter with a 500 nm
cut-off wavelength, which fit within the cradle and illuminated the
microchip from an angle. The LED was powered with 3 V from an Agilent E364xA
DC power supply with an automated on/off function controlled with a MATLAB
script. It was also determined that the blue LED could be adequately powered
by a standard 3 V lithium coin battery, but the DC power supply was used for
the purpose of PC control.

A Nikon ECLIPSE Ni fluorescent microscope with an InGaAs camera (Princeton Instruments, NIRvana TE 640), 785 nm, 100 mW/cm² laser excitation, 1,000 nm long-pass filter (Thorlabs), 800 nm short-pass filter (Thorlabs), and 805 nm cut-on long-pass dichroic mirror (Thorlabs) was used for NIR-II fluorescent microscopy imaging.