Rhslpi

rhSLPI was purchased from Sino Biological, Inc. (Beijing, China). Medium 200 (M-200-500), Low Serum Growth Supplement kit (S003K-LSGS) and trypsin-EDTA were purchased from Thermo Fisher Scientific, Inc. (Gibco; Waltham, MA, USA). The LDH liquid-UV assay kit was obtained from Human (Wiesbaden, Germany), and MTT was purchased from Ameresco, Inc. (Solon, OH, USA). Antibodies against total p38 (sc-728), phosphorylated-p38 (sc-17852-R), total Akt (sc-8312), phosphorylated-Akt (sc-293125), Bax (sc-6236), Bcl-2 (sc-783), and cleaved caspase 3 (sc-56053), β-actin (sc-130301), and the horseradish peroxidase-conjugated secondary antibody (sc-2004) were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). Other chemicals were purchased from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany).

All basic chemicals were purchased from Sigma-Aldrich; Merck KGaA (Darmstadt, Germany). M199 medium was obtained from Gibco; Thermo Fisher Scientific, Inc. (Waltham, MA, USA). For SDS-PAGE and western blot analysis, 30% polyacrylamide solution was purchased from Bio-Rad Laboratories, Inc. (Hercules, CA, USA) and the polyvinylidenedifluoride (PVDF) membrane was purchased from GE Healthcare Life Sciences (Little Chalfont, UK). The antibodies for the dual phosphorylated (p)-Thr180/Tyr182 form of p38 mitogen-activated protein kinase (MAPK; cat. no. sc-17852-R), total p38 MAPK (cat. no. sc-728), p-Akt (cat. no. sc-293125) and total Akt (cat. no. sc-8312) were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). Enhanced chemiluminescence (ECL) solution was purchased from GE Healthcare Life Sciences and collagenase type II was purchased from Worthington Biochemical Corporation (Lakewood, NJ, USA). rhSLPI was purchased from Sino Biological, Inc. (Beijing, China).