The cloning strain and the expression strain were E. coli DH5α and E. coli Rosetta (DE3), respectively. Plasmid pE-SUMO was employed as an expression vector with a 6 × His tag and a SUMO tag upstream of the multiple cloning sites. Heparin sodium salt (185 U/mg) was purchased from Solarbio company (Beijing, China). DNA polymerases, restriction enzymes, and T4 ligase were purchased from ThermoFisher (USA). The His GraviTrap TALON was purchased from GE Health (Uppsala,Sweden).
Heparin sodium salt
Manufactured by Solarbio
Sourced in China
Heparin sodium salt is a natural anticoagulant used as a laboratory reagent. It functions as an inhibitor of the blood coagulation cascade by binding to antithrombin III, thereby enhancing its activity and preventing the formation of thrombin and fibrin clots.
Automatically generated - may contain errors
Lab products found in correlation
T4 ligase, by Thermo Fisher Scientific (1 mentions)
His gravitrap talon, by GE Healthcare (1 mentions)
Streptozotocin (stz), by Santa Cruz Biotechnology (1 mentions)
Alpha glucosidase enzyme from saccharomyces cerevisiae, by Merck Group (1 mentions)
C57bl 6j background, by Jackson ImmunoResearch (1 mentions)
Deoxyribonuclease 1, by Worthington (1 mentions)
Hank s balanced salt solution, by Thermo Fisher Scientific (1 mentions)
Collagenase type 2, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Solarbio (1 mentions)
Trypsin, by Beyotime (1 mentions)
U 2910, by Hitachi (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Perchloric acid, by Merck Group (1 mentions)
Hplc grade methanol, by Tedia (1 mentions)
Acetonitrile, by Tedia (1 mentions)
Epigallocatechin gallate (egcg), by Chengdu Biopurify Phytochemicals (1 mentions)
4 protocols using heparin sodium salt
1
Cloning and Expression in E. coli
2
Alpha-Glucosidase Inhibition Assay
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The chemicals used for the experiments were all fresh and of analytical grade. Yeast extract (YE, Oxoid), magnesium sulfate (MgSO4, Bendosen), dipotassium phosphate (K2HPO4, Bendosen), mono-potassium phosphate (KH2PO4, Bendosen), ammonium chloride (NH4Cl, Bendosen), and D (+)- Glucose (R&M Chemicals). Alpha-glucosidase enzyme from Saccharomyces cerevisiae (Sigma-Aldrich), 4-Nitrophenyl glucopyranoside (pNPG, Sigma-Aldrich), heparin sodium salt (Solarbio), and Streptozotocin (Santa Cruz) were purchased in lyophilized powder form.
3
Isolation and Culture of Mouse Aortic Cells
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Collagenase Type II was purchased from Sigma-Aldrich (Sigma-Aldrich, Cat#C6885-100MG). Deoxyribonuclease I was purchased from Worthington (Worthington, Cat#LS002139). Trypsin was purchased from Beyotime (Beyotime, Cat#C0205). Other reagents used in this study include Hank’s Balanced Salt Solution (Thermo Fisher Scientific, Cat#14025092), PBS (Solarbio, Cat#P1000), Heparin sodium salt (Solarbio, Cat#H8060), Fetal bovine serum (HyClone, Cat#SV30087.03).
All mouse studies were approved by the Institutional Animal Care and Use Committee of Soochow University, and all protocols complied with institutional guidelines. ApoE-/- and wild-type mice (C57BL/6 J background) from the Jackson Laboratories (Bar Harbor, USA) were kept as previously described [2 (link)].
All mouse studies were approved by the Institutional Animal Care and Use Committee of Soochow University, and all protocols complied with institutional guidelines. ApoE-/- and wild-type mice (C57BL/6 J background) from the Jackson Laboratories (Bar Harbor, USA) were kept as previously described [2 (link)].
4
Oxidative Preparation of Tea Polyphenols
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Oxidative tea polyphenols was prepared in laboratory as described (Huang et al., 2014 (link)): EGCG (the purity is more than 98%) aqueous solution was oxidized with atmosphere oxygen at 37°C under alkaline conditions until the A230 nm/A520 nm (determined by spectrophotometer, HITACHI, U-2910, the optical path length is 1.0 cm, sample concentration is 500 mg L-1) equaled 1.40 (the value of the initial tea polyphenols sample is 10.90). Then, the solution was dried by lyophilization.
Atenolol was purchased from Micxy Chemical Co., Ltd (Chengdu, China), EGCG (purity >98%) was purchased from Chengdu Biopurify Phytochemicals Ltd (Chengdu, China). Heparin sodium salt was purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China). Trichloroacetic acid, NaOH, and KH2PO4 were purchased from Aladdin (Shanghai, China). Perchloric acid and Hydrochloric acid were purchased from Sigma–Aldrich (Shanghai, China). HPLC-grade methanol and acetonitrile purchased from Tedia Co. Inc. Deionized water was prepared using a purifier (FST-UV-20, Shanghai Fushite instrument equipment Co. Ltd. Shanghai, China). Blank plasma was obtained from Kunming Blood Center (Kunming, China).
Atenolol was purchased from Micxy Chemical Co., Ltd (Chengdu, China), EGCG (purity >98%) was purchased from Chengdu Biopurify Phytochemicals Ltd (Chengdu, China). Heparin sodium salt was purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China). Trichloroacetic acid, NaOH, and KH2PO4 were purchased from Aladdin (Shanghai, China). Perchloric acid and Hydrochloric acid were purchased from Sigma–Aldrich (Shanghai, China). HPLC-grade methanol and acetonitrile purchased from Tedia Co. Inc. Deionized water was prepared using a purifier (FST-UV-20, Shanghai Fushite instrument equipment Co. Ltd. Shanghai, China). Blank plasma was obtained from Kunming Blood Center (Kunming, China).
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