Adaptis a1000

An original method of growing peas in a hydroponic gnotobiotic system was applied [29 (link)]. Briefly, seeds of cv. Sparkle and E107 (brz) were surface-sterilized and scarified by treatment with 98% H₂SO₄ for 20 min, rinsed with sterile tap water, and germinated in Petri dishes for three days at 25 °C. Then, ten seedlings were transferred to each polypropylene pot (OS140BOX, Duchefa, Netherlands) containing 250 mL of sterile nutrient solution (µM): KNO₃, 1200; Ca(NO₃)₂, 60; MgSO₄, 250; KCl, 250; CaCl₂, 60; Fe-tartrate, 12; H₃BO₃, 2; MnSO₄, 1; ZnSO₄, 3; NaCl, 6; Na₂MoO₄, 0.06; CoCl₂, 0,06; CuCl₂, 0.06; NiCl₂, 0,06; pH = 4.7. The nutrient solution was supplemented or not with 80 µM AlCl₃×6H₂O and/or inoculated with bacteria in a final concentration of 10⁵ cells mL⁻¹. Non-supplemented solution was used as a control treatment. Plants were cultivated for 10 days in a growth chamber (ADAPTIS-A1000, Conviron, Isleham, UK) with 200 µmol of quanta m⁻² s⁻¹, a 12 h photoperiod, and minimum/maximum temperatures of 18 °C/23 °C. Then, the biomass of individual plants was determined, and the pH of the nutrient solution was measured using the pH meter F20 (Mettler-Toledo, Schwerzenbach, Switzerland). Five experiments with one pot per treatment were performed.

Mango (Mangifera indica cv. Zill) fruits were obtained from South Subtropical Crops Research Institute (SSCRI) in Zhanjiang, China. Fruitlets were bagged with double layers yellow-black paper bags (Qingdao Kobayashi Co., Ltd., Qingdao, China) at 20 days after full bloom to block out all the light. Green mature fruits (130 days after full bloom) were harvested with bags, transported to the lab, and debagged for postharvest light treatment in plant growth chambers (Conviron, Adaptis A 1000, Winnipeg, Canada). 180 unblemished fruits with uniformed size were divided into two groups, with half fruits subjected to mimic sunlight treatment (mixture of 4.5 μW•cm^-2 UV-B and 16 W•m⁻² white light) and the rest retained in darkness as control. The relative humidity and temperature were 80% and 17°C, respectively. All the conditions for treatment were according to our previous patent (Qian et al., 2022 ). 30 fruits were regarded as one biological replicate. Fruit peel of 5 fruits per replicate was collected at 0, 6, 24, 72, 144, and 240 hours of light exposure for metabolomic and gene expression analyses. For fruit peel sampling, the exposed side was sampled by a peeler for light-treated fruit, and the up-side fruit peel was sampled for control fruit. Fruit peel was sampled as thin as possible to ensure the minimum collection of flesh.

The soybean cultivars Harosoy, Williams 82 and Dongnong 50 were used in this study. All plants were grown in a growth chamber (Conviron ADAPTIS-A1000, Canada) at a consistent temperature of 25°C and an average photon flux of 300 µmol m⁻²s⁻¹, supplied by T5 fluorescent lamps. Day length regimes were 12L/12D for SD and 16L/8D or 18L/6D for LD. Tissue-specific expression was analyzed using the cultivar Harosoy grown under SD. Total RNA was isolated from trifoliate leaves, shoot apices, roots, flowers, flower buds, and roots. For the temporal expression analysis, pieces of young, fully developed trifoliate leaves and shoot apices were bulk sampled at 4 hours after dawn from 4 individual plants grown under SD every five days from 10 DAE until 25 DAE. The trifoliate leaves and shoot apices from 4 plants of both transgenic and untransformed lines were bulk sampled at 4 hours after dawn at 20 DAE under the LD condition and stored until total RNA extraction.