Macrophages were harvested from the peritoneal cavity of 8-week-old male WT and Tgfbr1G188V/+ mice at 3 days after injection of thioglycollate (Yamaba et al., 2015 (link)) and seeded on 6-well plates. After 2 h of incubation, non-adherent cells were washed out and the remaining cells were subjected to further analysis. The peritoneal macrophages were stimulated with 1 Uμg/ml P. gingivalis lipopolysaccharide (LPS) (InvivoGen, Inc., San Diego, CA, United States) for the indicated times and then total RNA was isolated for quantitative PCR analysis.
P gingivalis lipopolysaccharide lps
Manufactured by InvivoGen
Sourced in United States
P. gingivalis lipopolysaccharide (LPS) is a laboratory product derived from the cell wall of the bacterium Porphyromonas gingivalis. It is a known Toll-like receptor 4 (TLR4) agonist and can be used to stimulate immune responses in in vitro and in vivo studies.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using p gingivalis lipopolysaccharide lps
1
Isolation and Stimulation of Peritoneal Macrophages
2
Macrophage Polarization by P. gingivalis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
BMDMs stimulated with 1 μg mL-1P. gingivalis lipopolysaccharide (LPS) (InvivoGen, San Diego, CA, USA) plus 20 ng mL-1 interferon-γ (IFN-γ) (Gibco) or 20 ng -1 IL-4 (Gibco) were used as M1 and M2 positive controls, respectively, for gating in flow cytometry. Cells infected with P. gingivalis with or without Stattic, the positive control groups, and BMDMφ were collected, treated with anti-mouse CD16/32 (TruStain FcX™ PLUS, Biolegend, #156603, San Diego, CA, USA), and then incubated with anti-mouse CD86-fluorescein isothiocyanate (FITC) antibody (clone GL-1, Biolegend, #105005) and anti-mouse CD206-allophycocyanin (APC) antibody (clone C068C2, Biolegend, #141707). according to the manufacturer's instructions. The labeled cells were then analyzed on a Beckman Coulter FC500 flow cytometer (Beckman Coulter Pty Ltd., NSW, Australia), and data were analyzed using FlowJo (v10, BD, Ashland, USA).
3
Topical Application of Microbial Agents in Maxillary Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We have developed a method to apply chemical therapeutic agents topically to the mouse maxillary tissue [24 (link), 78 (link)]. We used the maxillary topical application (MTA) model to apply oral microbial components [8 (link)]. The present study used the MTA model using 1 μg/ml unmethylated CpG oligonucleotide (CpG ODN: InvivoGen, San Diego, CA) or P. gingivalis lipopolysaccharide (LPS: InvivoGen). First, a custom-made oral appliance was fabricated using clear dental resin, covering the maxillary/palatal tissue between the molars. Mice were anesthetized and placed on a supine position. CpG ODN (3 μl) or LPS (3 μl) was pipetted over the maxillary tissue and the oral appliance was placed to hold the solution with a bite block for 1 hr in an anesthetization chamber. Mice were then transferred to the operation table and the oral appliance and bite block were removed. In general, there was no remaining solution in the mouth. Mice were returned to the cage in the vivarium. On Day 4 of the MTA, mice were euthanized by 100% CO2 inhalation and the maxillary tissue was harvested and fixed with 10% buffered formalin for histological evaluation.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!