Fluorometric intracellular ros kit mak143

The ROS production was measured
using the Fluorometric Intracellular ROS kit MAK143 (Sigma-Aldrich,
St. Louis, MO) according to the manufacturer’s protocol. In
brief, SH-SY5Y cells were seeded in black polystyrene 96-well plates
for 24 h and then treated in the absence or in the presence of 5 μM
Aβ40 oligomers. The ROS production was monitored over time by
measuring the emission of fluorescence at 520 nm (excitation at 490
nm) at 37 °C using a plate reader (BMGLabtech, Aylesbury, U.K.).

ROS production was measured using the Fluorometric Intracellular ROS kit MAK143 (Sigma-Aldrich, St. Louis, MO) following the manufacturer’s protocol. Briefly, SH-SY5Y cells were seeded in black polystyrene 96-well plates for 24 h, followed by treatment with 1 μM of samples containing Aβ40 in the absence or presence of 10 molar equivalents of DOPAL after a 20 h incubation. The ROS production was monitored over time exploiting the fact that ROS species react with a fluorogenic sensor in the cytoplasm, resulting in a fluorometric product whose amount is proportional to the amount of ROS present. We thus measured the emission of fluorescence at 520 nm (excitation at 490 nm) at 37 °C using a plate reader (BMG Labtech, Aylesbury, UK).