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αrabbit hrp

Manufactured by Dianova
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αRabbit-HRP is a horseradish peroxidase (HRP) conjugated secondary antibody that binds to rabbit primary antibodies. It can be used for detection and visualization of rabbit-derived antigens in various immunoassay techniques.

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Lab products found in correlation

α flag hrp, by Merck Group (2 mentions) αmouse hrp, by Dianova (2 mentions) Normal mouse igg, by Santa Cruz Biotechnology (1 mentions) Normal rabbit igg, by Santa Cruz Biotechnology (1 mentions) Ab76115, by Abcam (1 mentions) Ab6160, by Abcam (1 mentions) Ab6663, by Abcam (1 mentions) Sc 2025, by Santa Cruz Biotechnology (1 mentions) Mms 101r, by Fortrea (1 mentions) Imagequant las 4000, by GE Healthcare (1 mentions) Ab13970, by Abcam (1 mentions) Pvdf membrane, by Roche (1 mentions) α flag, by Merck Group (1 mentions) Ab6753, by Abcam (1 mentions) Veriblot, by Abcam (1 mentions)

2 protocols using αrabbit hrp

1

Comprehensive Antibody Toolkit for Synaptic Proteins

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Primary antibodies: αSynCAM-Biotin (chicken, CM004-6, MBL), αSynCAM (rabbit, PA3-16744, Thermo Scientific), αMPP2 (rabbit, ab97290, Abcam), αPSD-95 (rabbit, ab76115, Abcam), αPSD95 (mouse, 75-028, NeuroMab), αGluA2 (mouse, 75-002, NeuroMab), αStargazin/TARPγ2 (mouse, 73-242, NeuroMab) αStargazin (rabbit, 2503S, Cell Signaling), αTubulin (rat, ab6160, Abcam), αFlag-HRP (mouse, A8592, Sigma), αHA (mouse, MMS-101R, Covance), αHA (rabbit, H6928, Sigma), αMyc (mouse, 631206, Clontech), αMYC (rabbit, 2272S, Cell Signalling), αHSV (rabbit, ab3414, Abcam), αGFP (goat, ab6663, Abcam), normal mouse IgG (sc-2025, Santa Cruz), normal rabbit IgG (sc-2027, Santa Cruz), normal chicken IgY (sc-2718, Santa Cruz). Secondary antibodies: αMouse-HRP (115-035-003, Dianova), αRabbit-HRP (111-035-003, Dianova), αRat-HRP (sc-2032, Santa Cruz), αGoat-HRP (sc-2020, Santa Cruz).
Rademacher N., Schmerl B., Lardong J.A., Wahl M.C, & Shoichet S.A. (2016). MPP2 is a postsynaptic MAGUK scaffold protein that links SynCAM1 cell adhesion molecules to core components of the postsynaptic density. Scientific Reports, 6, 35283.
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2

Western Blot Analysis of GABA Receptor Subunits

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Lysates and beads were boiled in 2x SDS-sample buffer (8% SDS, 40% glycerol, 0.25 M Tris pH 6.8, 20% β-mercaptoethanol) for 5 minutes, separated on a 10% SDS-PAGE, and blotted onto PVDF membranes (Roche). Membranes were blocked with 5% skim milk/PBST. Primary antibodies were diluted in the blocking buffer and applied over night at 4°C, followed by 3 washes with PBST and 1 hour incubation with appropriate secondary antibodies. HRP signals were detected using Western Lightning chemiluminescent substrates (Perkin Elmer, USA) with a luminescent image analyser (ImageQuant LAS4000, GE Healthcare).
Primary antibodies: αGABAAR α1 (mouse, 75–136, NeuroMab), αFlag (mouse, F1804, Sigma), αFlag-HRP (mouse, A8592, Sigma), αGFP (chicken, ab13970, Abcam), αGST (mouse, 75–148, NeuroMab), and αHA (rabbit, H6928, Sigma).
Secondary antibodies: αMouse-HRP (115-035-003, Dianova), αMouse-native-IgG (Veriblot, 131368, Abcam), αRabbit-HRP (111-035-003, Dianova), and αChicken-HRP (ab6753, Abcam).
Schmerl B., Gimber N., Kuropka B., Stumpf A., Rentsch J., Kunde S.A., von Sivers J., Ewers H., Schmitz D., Freund C., Schmoranzer J., Rademacher N, & Shoichet S.A. (2022). The synaptic scaffold protein MPP2 interacts with GABAA receptors at the periphery of the postsynaptic density of glutamatergic synapses. PLoS Biology, 20(3), e3001503.
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