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Ap conjugated mach2 mouse polymer

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The AP-conjugated MACH2 Mouse Polymer is a laboratory reagent used in immunohistochemistry and other immunoassay applications. It is a polymer-based detection system that utilizes an alkaline phosphatase (AP) enzyme label to amplify the signal from the primary antibody-antigen interaction. The AP-conjugated MACH2 Mouse Polymer provides a sensitive and reliable method for the visualization of target proteins in tissue sections or other biological samples.

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Lab products found in correlation

Dab chromogen solution, by Agilent Technologies (2 mentions) Anti mouse cd45 antibody, by BD (2 mentions) Lyve 1, by Agilent Technologies (2 mentions) Envision dual link hrp polymer, by Agilent Technologies (2 mentions) Vulcan fast red chromogen, by Biocare Medical (2 mentions) Human cd45 antibody, by Agilent Technologies (2 mentions) Mouse lyve 1, by Abcam (2 mentions) Anti rat biotinylated antibody, by Agilent Technologies (2 mentions) Streptavidin hpr conjugated solution, by Agilent Technologies (2 mentions) Lyve 1, by Abcam (2 mentions) 3 3 diaminobenzidine (dab), by Agilent Technologies (2 mentions)

2 protocols using ap conjugated mach2 mouse polymer

1

Immunohistochemical Staining of CD45 and Lyve-1

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For single staining with the human CD45 antibody (Dako) or mouse lyve-1 (Abcam, San Fransisco, CA), the samples were incubated with antibody, then rinsed and a secondary reagent, AP-conjugated MACH2 Mouse Polymer (BioCare Medical, Concord, CA), RT for CD45 staining or EnVision + Dual Link- HRP Polymer (Dako) for lyve-1 staining was applied before development with Vulcan Fast Red Chromogen (BioCare Medical) or DAB+ (Dako). For dual staining of mouse and human CD45, anti-mouse CD45 antibody (BD Pharmingen, San Jose, CA) was applied, followed by a secondary rabbit anti-rat biotinylated antibody (Dako) and Streptavidin-HPR conjugated solution (Dako). The CD3, lyve-1 (Abcam), and CD19 (MyBiosource, San Diego, CA) antibodies were applied at the appropriate dilution, followed by HRP-conjugated EnVision + Dual Link System (Dako). In all cases, primary staining was developed using DAB+ chromogen solution (Dako).
Morton J.J., Bird G., Keysar S.B., Astling D.P., Lyons T.R., Anderson R.T., Glogowska M.J., Estes P., Eagles J.R., Le P.N., Gan G., McGettigan B., Fernandez P., Padilla-Just N., Varella-Garcia M., Song J.I., Bowles D.W., Schedin P., Tan A.C., Roop D.R., Wang X.J., Refaeli Y, & Jimeno A. (2015). XactMice: humanizing mouse bone marrow enables microenvironment reconstitution in a patient-derived xenograft model of head and neck cancer. Oncogene, 35(3), 290-300.
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2

Immunohistochemical Staining of CD45 and Lyve-1

Check if the same lab product or an alternative is used in the 5 most similar protocols
For single staining with the human CD45 antibody (Dako) or mouse lyve-1 (Abcam, San Fransisco, CA), the samples were incubated with antibody, then rinsed and a secondary reagent, AP-conjugated MACH2 Mouse Polymer (BioCare Medical, Concord, CA), RT for CD45 staining or EnVision + Dual Link- HRP Polymer (Dako) for lyve-1 staining was applied before development with Vulcan Fast Red Chromogen (BioCare Medical) or DAB+ (Dako). For dual staining of mouse and human CD45, anti-mouse CD45 antibody (BD Pharmingen, San Jose, CA) was applied, followed by a secondary rabbit anti-rat biotinylated antibody (Dako) and Streptavidin-HPR conjugated solution (Dako). The CD3, lyve-1 (Abcam), and CD19 (MyBiosource, San Diego, CA) antibodies were applied at the appropriate dilution, followed by HRP-conjugated EnVision + Dual Link System (Dako). In all cases, primary staining was developed using DAB+ chromogen solution (Dako).
Morton J.J., Bird G., Keysar S.B., Astling D.P., Lyons T.R., Anderson R.T., Glogowska M.J., Estes P., Eagles J.R., Le P.N., Gan G., McGettigan B., Fernandez P., Padilla-Just N., Varella-Garcia M., Song J.I., Bowles D.W., Schedin P., Tan A.C., Roop D.R., Wang X.J., Refaeli Y, & Jimeno A. (2015). XactMice: humanizing mouse bone marrow enables microenvironment reconstitution in a patient-derived xenograft model of head and neck cancer. Oncogene, 35(3), 290-300.
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