Sn 38

SN-38 was purchased from Tokyo Chemical Industry Co. Ltd. (Tokyo, Japan); and CPT-11, from Yakult Honsha Co., Ltd. (Tokyo, Japan).

Camptothecin and SN-38 were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Ko143 was purchased from Sigma Aldrich Co. LLC (St. Louis, MO, USA). Verapamil and rifampicin were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Baicalin was purchased from AdooQ Bioscience (Irvine, CA, USA), and Cell Quanti-Blue^TM Cell Viability Assay Kit was purchased from Bio Assay Systems (Hayward, CA, USA). All other reagents were of high-purity analytical or high-performance liquid chromatography (HPLC)-grade. Pooled human serum from healthy subjects (normal serum) was purchased from Merck Millipore Ltd. (Billerica, MA, USA), and pooled human serum from patients with ESKD (uremic serum) was obtained from more than 400 patients with ESKD undergoing hemodialysis at Shirasagi Hospital (Osaka, Japan). Blood samples were collected immediately before hemodialysis in a non-invasive manner. Normal and uremic serum were deproteinized by 3-times volume of methanol as much as serum and evaporated under a nitrogen stream at 50 °C; the resulting serum residues, normal serum residue (NSR) and uremic serum residue (USR), were used in the study^{3 (link)}. The present study was approved by the ethics committees of Shirasagi Hospital (IRB number J2012013) and Kyoto Pharmaceutical University (IRB number 08-04).

CPT-11 hydrochloride was purchased from Carbosynth Limited (Berkshire, UK). SN-38 was purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Celecoxib was purchased from Combi-Blocks, Inc. (San Diego, CA, USA). d-sorbitol and TRI reagent were purchased from Sigma-Aldrich Corp. (St. Louis, MO, USA). Lactic acid and d-MEM medium were purchased from Wako Pure Chemicals (Osaka, Japan). WST-1 was purchased from Roche Diagnostics (Indianapolis, IN, USA). Rabbit anti-rat AQP3 antibody, rabbit anti-human AQP1 antibody, and rabbit anti-rat AQP4 antibody were purchased from Alomone Labs (Jerusalem, Israel). Rabbit anti-rat AQP8 antibody was purchased from Alpha Diagnostic Inc. (San Antonio, TX, USA). Alexa Fluor 488 donkey anti-rabbit IgG was purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA). Purified anti-β-actin antibody was purchased from BioLegend Inc. (San Diego, CA, USA). Donkey anti-rabbit IgG-HRP antibody and ECL prime Western blotting detection reagents were purchased from GE Healthcare (Chalfont St. Giles, UK). All primers for real-time PCR were purchased from Hokkaido System Science Co., Ltd. (Hokkaido, Japan). A high-capacity cDNA reverse transcription kit was purchased from Applied Biosystems (Foster City, CA, USA). SsoAdvanced SYBR green supermix was purchased from Bio-Rad Laboratories (Hercules, CA, USA).