Fluorojade c

Manufactured by Immunological Sciences

Sourced in Italy

FluoroJade-C is a fluorescent dye used for the histological detection of degenerating neurons in fixed, paraffin-embedded tissue sections. It provides a sensitive and specific marker for the identification of neuronal degeneration.

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Lab products found in correlation

Frosted microscope slides, by Thermo Fisher Scientific (1 mentions) Dpx mountant, by Merck Group (1 mentions)

2 protocols using fluorojade c

FluoroJade-C Brain Labeling Protocol

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Cryostat brain sections were mounted on frosted microscope slides and pretreated for 5 min by immersion in 80% EtOH and 1% NaOH, followed by 2 min in EtOH 70% and 2 min washing in distilled water. These sections were then incubated for 10 min in 0.06% KMnO₄ and rinsed in distilled water for 3 min, before incubation for 10 min in a solution of 0.0001% FluoroJade-C (Immunological Sciences, Rome, Italy) and 0.1% acetic acid. After three washes in distilled water for 1 min, slides were dried, dehydrated with xylene, and coverslipped with DPX mountant for microscopy (Sigma, Saint Louis, MO, USA).

Verlengia G., Miyagawa Y., Ingusci S., Cohen J.B., Simonato M, & Glorioso J.C. (2017). Engineered HSV vector achieves safe long-term transgene expression in the central nervous system. Scientific Reports, 7, 1507.

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Fluoro-Jade C Staining of Brain Sections

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Brain sections were mounted on frosted microscope slides (Thermo Fisher Scientific) and pretreated for 5 min in 80% EtOH/1% NaOH, 2 min in 70% EtOH, and 2 min in distilled water. Sections were then incubated for 10 min in a 0.06% KMnO₄ solution and rinsed in distilled water for 3 min before incubation in a 0.0001% solution of Fluoro-Jade C (Immunological Sciences, IS-0012) in 0.1% acetic acid for 10 min. After three washes in distilled water for 1 min, slides were dried, dehydrated with xylene, and coverslipped with DPX mountant (Sigma) for microscopy.

Miyagawa Y., Verlengia G., Reinhart B., Han F., Uchida H., Zucchini S., Goins W.F., Simonato M., Cohen J.B, & Glorioso J.C. (2017). Deletion of the Virion Host Shut-off Gene Enhances Neuronal-Selective Transgene Expression from an HSV Vector Lacking Functional IE Genes. Molecular Therapy. Methods & Clinical Development, 6, 79-90.

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