4 nitrophenol α d glucopyranoside

The seeds of M. oleifera were hand-collected from local trees near La Coronela neighborhood, La Habana, Cuba. The seeds were washed and stored at −80 °C until use. Coomassie Blue G-250, N,N,N′,N′-tetramethylethylenediamine (TEMED), sodium chloride, tris (hydroxymethyl) aminomethane, sodium dodecyl sulphate (SDS), β-mercaptoethanol (βME), bovine serum albumin (BSA), l-pyroglutamyl-l-phenylalanyl-l-leucine-p-nitroanilide (PFLNA), Trypsin, 4-nitrophenol-α-d-glucopyranoside (PNPG) and α-glucosidase were purchased from Sigma-Aldrich (San Luis, MO, USA). Glyoxyl-agarose was delivered from FlukaTM. All other chemicals used in this work were of analytical grade and purchased from Sigma-Aldrich (San Luis, MO, USA) (unless otherwise specified).

The seeds of the Yellow Bell Pepper (Capsicum annuum) were hand-collected from local farmers around La Plata, Buenos Aires, Argentina. The seeds were processed as previously described elsewhere [11 (link)]. The reagents sodium chloride, Coomassie Blue G-250, N,N,N′,N′-tetramethylethylenediamine (TEMED), tris (hydroxymethyl) aminomethane, sodium dodecyl sulphate (SDS), bovine serum albumin (BSA), β-mercaptoethanol (β ME), Nα-benzoyl-DL-arginine-p-nitroanilide (BApNA), and 4-nitrophenol-α-D-glucopyranoside (PNPG) were purchased from Sigma-Aldrich (San Luis, MO, USA). The proteases used in this study, such as Trypsin and α-Glucosidase, were obtained from Sigma-Aldrich. To perform PI purification, a Glyoxyl-agarose resin was obtained from FlukaTM. Fluconazole was purchased from Signa-Aldrich. All chemicals and reagents used in this study were of analytical grade, unless otherwise specified.